YAP2基因对大鼠H9c2心肌细胞凋亡及TNF-α和IL-6水平的影响

doi:10.3760/cma.j.issn.1007-1245.2024.14.003

摘要/Abstract

摘要：

目的　探讨沉默Yes相关蛋白2（YAP2）基因表达对H₂O₂诱导的大鼠H9c2心肌细胞活力、凋亡率及炎症因子的影响及机制。方法　本实验于2023年5月至12月实施。实验细胞分为4个处理组，即正常对照组（Control组）、H₂O₂组、si-YAP2+H₂O₂组、NC+H₂O₂组。通过MTT、流式细胞术及Western blotting分别检测4组细胞活力、凋亡率及YAP2、Bcl-2、Bax、p-AKT蛋白表达；酶联免疫吸附试验（ELISA）试剂盒检测各组细胞培养液中肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6含量。采用单因素方差分析、SNK-q检验。结果　H₂O₂组、si-YAP2+H₂O₂组YAP2表达量分别为（0.716±0.056）、（0.204±0.018），差异有统计学意义（P<0.05）。H₂O₂组H9c2心肌细胞活力低于si-YAP2+H₂O₂组和Control组，细胞凋亡率高于si-YAP2+H₂O₂组和Control组，差异均有统计学意义（均P<0.05）。H₂O₂组和NC+H₂O₂组H9c2心肌细胞活力、细胞凋亡率差异均无统计学意义（均P>0.05）。H₂O₂组Bcl-2、p-AKT表达量低于si-YAP2+H₂O₂组和Control组，Bax表达量高于si-YAP2+H₂O₂组和Control组，差异均有统计学意义（均P<0.05）。H₂O₂组和NC+H₂O₂组Bcl-2、p-AKT、Bax表达量差异均无统计学意义（均P>0.05）。H₂O₂组TNF-α和IL-6含量高于si-YAP2+H₂O₂组和Control组（均P<0.05）。H₂O₂组和NC+H₂O₂组TNF-α和IL-6含量差异均无统计学意义（均P>0.05）。结论　沉默YAP2基因表达可能通过降低心肌细胞凋亡及炎症因子TNF-α和IL-6含量，从而降低心血管疾病发生。

关键词:

心肌细胞, YAP2基因, 凋亡, 免疫, PI3K/AKT信号通路

Abstract:

Objective　To investigate the effect of Yes-associated protein 2 (YAP2) gene silencing on rat H9c2 cardiomyocyte viability, apoptosis rate, and inflammatory factors induced by H₂O₂. Methods　The experiment was conducted from May to December 2023. The experiment cells were divided into four groups: a normal control group, a H₂O₂ group, a si-YAP2+H₂O₂ group, and a NC+H₂O₂ group. MTT, flow cytometry, and Western blotting were used to detect the cell viability, apoptosis rate, and the expressions of YAP2, Bcl-2, Bax, and p-AKT protein in the four groups, and the contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the cell culture medium of each group were detected by enzyme-linked immunosorbent assay (ELISA) kit. One-way analysis of variance and SNK-q test were used. Results　The expression levels of YAP2 in the H₂O₂ group and the si-YAP2+H₂O₂ group were (0.716±0.056) and (0.204±0.018), respectively, with a statistically significant difference (P<0.05). The viability of H9c2 cardiomyocytes in the H₂O₂ group was significantly lower than that in the si-YAP2+H₂O₂ group and the normal control group, while the apoptosis rate was significantly higher, with statistically significant differences (all P<0.05). There were no statistically significant differences in the viability or apoptosis rate of H9c2 cardiomyocytes between the H₂O₂ group and the NC+H₂O₂ group (both P>0.05). The expression levels of Bcl-2 and p-AKT in the H₂O₂ group were significantly lower than those in the si-YAP2+H₂O₂ group and the normal control group, while the expression level of Bax was significantly higher, with statistically significant differences (all P<0.05). There were no statistically significant differences in the expression levels of Bcl-2, p-AKT, and Bax between the H₂O₂ group and the NC+H₂O₂ group (all P>0.05). The contents of TNF-α and IL-6 in the H₂O₂ group were significantly higher than those in the si-YAP2+H₂O₂ group and the normal control group (all P<0.05). There were no statistically significant differences in the contents of TNF-α and IL-6 between the H₂O₂ group and the NC+H₂O₂ group (both P>0.05). Conclusion　Silencing the expression of YAP2 gene may reduce the occurrence of cardiovascular diseases by reducing the apoptosis of cardiomyocytes and the contents of inflammatory factors TNF-α and IL-6.

Key words:

Cardiomyocytes, YAP2 gene, Apoptosis, Immunity, PI3K/AKT signaling pathway

黄毅王琮翰.

YAP2基因对大鼠H9c2心肌细胞凋亡及TNF-α和IL-6水平的影响 [J]. 国际医药卫生导报, 2024, 30(14): 2297-2302.

Huang Yi, Wang Conghan.

Effects of YAP2 gene on rat H9c2 cardiomyocyte apoptosis and TNF-α and IL-6 levels [J]. International Medicine and Health Guidance News, 2024, 30(14): 2297-2302.

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