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Effect of miR-34a based on AKT/mTOR pathway on osteoarthritis rats and
its mechanism
- Zhou Lun, Ding Yuan, Lyu Zhou
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2022, 28(21):
2990-2994.
DOI: 10.3760/cma.j.issn.1007-1245.2022.21.007
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Asbtract
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Objective To explore the effect of microrNA-34A (miR-34a) on osteoarthritis in
rats based on the AKT/mTOR pathway and its mechanism. Methods The study was form February 2020 to May 2021. In this study, 43 healthy
male SD rats were selected for prospective study, and they were 6-9 weeks old.
Among them, 10 rats were randomly selected as a blank group, and The remaining
33 rats were established as the osteoarthritis models. The 30 rats successfully
modeled were randomly divided into a model group, an up-regulated miR-34a
group, and a silenced miR-34a group, with 10 rats in each group. The expression
of miR-34a was detected by real-time polymerase chain reaction (RT-PCR), and
The levels of interleukin-1β (Il-1β), tumor necrosis factor-α, TNF-α,
interleukin-4 (IL-4), and interleukin-10 (IL-10) was detected by enzyme-linked
immunosorbent assay (ELISA). The expressions of phosphorylated protein kinase B
(pAKT) and phosphorylated mammalian target of rapamycin (pmTOR) were detected
by Western blot. F test was used for
the comparison between ≥3 groups, and
independent-sample t test was used
for the comparison between two groups. Results The expressions of
miR-34a in the blank group, the model group, the up-regulated miR-34a group,
and the silenced miR-34a group were (0.94±0.08), (2.39±0.26), (2.12±0.23), and
(1.54±0.17), rthe Makin scores (0.35±0.05), (4.13±0.19), (3.02±0.16), and
(1.15±0.09), the IL-1β levels (3.68±0.37), (7.51±1.23), (5.72±0.95), and
(4.63±0.72) ng/L, the TNF-α levels (37.45±4.85), (62.05±7.49), (56.26±6.25), and
(43.85±5.10) ng/L, the IL-4 levels (115.75±16.81), (50.43±9.42), (75.82±11.94),
and (93.85±13.86) pg/ml, the IL-10 levels (106.49±17.51), (60.85±10.23),
(76.81±13.07), and (91.05±15.02) pg/ml, the pAKT (1.00±0.01), (1.95±0.29),
(1.71±0.26), and (1.71±0.26), and the pmTOR (1.00±0.01), (1.89±0.24),
(1.56±0.25), and (1.25±0.15). Compared with those in the blank group, the
expression of miR-34a, Makin score, IL-1β, TNF-α, pAKT and pmTOR were higher in
than those in the model group, the up-regulated miR-34a group, and the silenced
miR-34a group, while IL-4 and IL-10 were lower those in the model group (F=25.290, P<0.001; F=91.260, P<0.001; F=14.144, P<0.001; F=13.077, P<0.001; F=15.525, P<0.001; F=17.580, P<0.001; F=16.080, P<0.001; and F=10.676, P<0.001). Compared with those in
the model group, the expression of miR-34a, Makin score, IL-1β, TNF-α, pAKT,
and pmTOR were lower than those in the up-regulated miR-34a group and the
silenced miR-34a group, while IL-4 and IL-10 were higher, with statistical
differences. Compared with those in the up-regulated miR-34a group, the
expression of miR-34a, Makin score, IL-1β, TNF-α, pAKT, and pmTOR were lower
than those in the miR-34a silencing group, while IL-4 and IL-10 were higher
than those in the miR-34a silencing group, with statistical differences (all P<0.05). Conclusion Silencing of miR-34a has an intervention effect in osteoarthritis rats,
and reduces the inflammatory response and the Makin score, and the mechanism of
action is related to the AKT / mTOR signaling pathway.
