关键词:

骨质疏松性骨折, 右归丸, 骨髓间充质干细胞, 愈合, JAK2/STAT3信号通路, 动物实验

Abstract:

Objective　To explore the effect and possible mechanism of Yougui Pills assisting bone marrow mesenchymal stem cells (BMSCs) on healing of osteoporotic fractures in rabbits. Methods　This study was from January to June 2023. Fifty rabbits were divided into a normal control group, a model control group, a Yougui pill group, a BMSCs group, and a combined treatment group, with 10 in each group. Osteoporosis fracture models were established in all groups except the normal control group, and corresponding drugs were given. After the treatment, the bone mineral density (BMD), trabecular number, trabecular thickness, trabecular dispersion, elastic modulus, stiffness, maximum stress, and maximum bearing capacity of femur were measured. The levels of Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) gene and protein in femur were determined by real time polymerase chain reaction and Western blotting. One-way analysis of variance and LSD-t test were used to analyze the data. Results　The BMD, number of bone trabeculae, thickness of bone trabeculae, elastic modulus, stiffness, maximum stress, maximum bearing capacity, and levels of JAK2 and STAT3 mRNA and protein of femurs in the model control group were lower than those in the normal control group (P<0.05), and the trabecular dispersion in the model control group was higher than that in the normal control group (all P<0.05). The BMD, number of bone trabeculae, thickness of bone trabeculae, elastic modulus, stiffness, maximum stress, maximum bearing capacity, and levels of JAK2 and STAT3 mRNA and protein of femurs in the Yougui pill group, the BMSCs group, and the combined treatment group were higher than those in the model control group (all P<0.05). The trabecular bone dispersions in the Yougui pill group, the BMSCs group, and the combined treatment group were lower than that in the model control group (all P<0.05). The femoral BMD [(1.76±0.13) g/cm³], trabecular number [(5.87±0.24) pieces/mm], trabecular thickness [(0.14±0.03) mm], femoral elastic modulus [(343.27±21.04) MPa], stiffness [(904.25±73.25) N/mm], maximum stress [(32.63±3.17) N/mm²], maximum bearing capacity [(358.85±13.02) N], and levels of JAK2 and STAT3 mRNA [(3.45±0.26) and (3.60±0.28)] and protein [(0.82±0.05) and (0.84±0.05)] in the combined treatment group were higher than those in the Yougui pills group [(1.41±0.15) g/cm³, (3.88±0.28) pieces/mm, (0.10±0.02) mm, (304.62±20.41) MPa, (698.52±69.55) N/mm, (25.34±3.22) N/mm², (297.34±13.24) N, (2.58±0.26), (2.29±0.23), (0.60±0.05), and (0.53±0.07)] and the BMSCs group [(1.47±0.14) g/cm³, (3.89±0.28) pieces/mm, (0.09±0.03) mm, (299.54±16.94) MPa, (720.33±69.48) N/mm, (23.24±3.13) N/mm², (289.38±13.13) N, (2.63±0.25), (2.30±0.24), (0.59±0.06), and (0.53±0.08)] (all P<0.05); the bone trabecular dispersion [(0.34±0.02) mm] in the combined treatment group was lower than those in the Yougui pills group [(0.42±0.03) mm] and the BMSCs group [(0.43±0.03) mm] (both P<0.05). Conclusions　Yougui pills assisting BMSCs can significantly promote the healing of osteoporotic fractures in rabbits; the mechanism may be related to the activation of the JAK2/STAT3 signaling pathway of femur in rabbits with osteoporotic fractures.

Key words:

Osteoporosis fractures, Yougui Pills, Bone marrow mesenchymal stem cells, Healing, JAK2/STAT3 signaling pathway, Animal experiment