复方骨肽注射液对髁突骨折后大鼠颌骨发育的影响

doi:10.3760/cma.j.issn.1007-1245.2023.17.018

国际医药卫生导报 ›› 2023, Vol. 29 ›› Issue (17): 2446-2451.DOI: 10.3760/cma.j.issn.1007-1245.2023.17.018

复方骨肽注射液对髁突骨折后大鼠颌骨发育的影响

蒋骞¹ 陈毅飞² 陈雅秋¹ 朱志丹¹ 张嘉杰¹ 孙晓婧³

¹桂林医学院附属口腔医院，桂林　541000；²桂林医学院药学院，桂林　541000；³桂林市口腔医院正畸科，桂林　541000

收稿日期:2023-03-22 出版日期:2023-09-01 发布日期:2023-09-21
通讯作者: 孙晓婧，Email：1870939206@qq.com
基金资助:
广西医疗卫生适宜技术开发与推广应用项目（S2022153）；桂林市科学研究与技术开发计划项目（2020011203-5）；桂林医学院中青年教职工科研能力提升项目（2018glmcy018）；大学生创新创业训练计划项目（30301022040）

Experimental study on the effect of ossotide on the development of jaw bone in rats after condylar fracture

Jiang Qian¹, Chen Yifei², Chen Yaqiu¹, Zhu Zhidan¹, Zhang Jiajie¹, Sun Xiaojing³

¹Affiliated Stomatological Hospital of Guilin Medical University, Guilin 541000, China; ²College of Pharmacy, Guilin Medical University, Guilin 541000, China; ³Department of Orthodontics, Guilin City Hospital of Stomatology, Guilin 541000, China

Received:2023-03-22 Online:2023-09-01 Published:2023-09-21
Contact: Sun Xiaojing, Email: 1870939206@qq.com
Supported by:
Guangxi Medical Health Suitable Technology Development and Promotion Application Project (S2022153); Guilin Scientific Research and Technology Development Plan Project (2020011203-5); Guilin Medical College Young and Middle-aged Faculty Research Ability Improvement Project (2018glmcy018); Innovation and Entrepreneurship Training Program for College Students (30301022040)

摘要/Abstract

摘要：

目的　观察复方骨肽注射液对单侧髁突颈部骨折大鼠的颌骨发育及其血清类胰岛素一号生长因子（IGF-1）及血小板衍生生长因子-BB（PDGF-BB）表达的影响。方法　实验于2019年10月25号开始。通过手术建立大鼠单侧髁突颈部骨折的动物模型，分别设立手术组、手术给药组（骨肽）和空白对照组，每组24只。术后手术给药组每天按0.9 mg/kg剂量注射复方骨肽注射液。然后分别于1、3、5、9周处死实验动物，每次每组处死6只。期间观察大鼠咬合对称性、下颌骨长度、下颌升支高度与血清中IGF-1、PDGF-BB含量的变化。采用方差分析、LSD检验。结果　单侧髁突骨折后，手术组正常侧下颌骨发育超过患侧下颌骨，下颌骨出现偏斜现象；手术给药组的下颌骨出现偏斜的程度较手术组轻，且X片上骨折的愈合加快。血清学结果显示，术后1周时手术组、手术给药组和空白对照组PDGF-BB分别为（104.21±15.01）ng/L、（241.69±22.49）ng/L、（406.47±26.88）ng/L，两两比较差异均有统计学意义（均P<0.001）；术后5周时手术组、手术给药组和空白对照组IGF-1分别为（2 109.95±181.74）ng/L、（2 714.89±180.21）ng/L、（2 499.27±186.04）ng/L，两两比较差异均有统计学意义（均P<0.05）。结论　单侧髁突颈部骨折和骨肽的使用都会影响处于生长高峰期的雄性SD大鼠下颌骨的生长发育及血清中PDGF-BB和IGF-1的含量。骨折后出现患侧下颌骨发育停滞或变慢，下颌骨偏斜，而注射复方骨肽后，骨折断端的愈合会加快，下颌骨偏斜程度会降低，且血清内两因子受到影响后的变化是动态的，不同时间点的含量不同。

关键词:

下颌骨, 髁突颈部骨折, 骨肽, 类胰岛素一号生长因子, 血小板衍生生长因子-BB, 动物实验

Abstract:

Objective　To observe the effects of compound ossotide injection on the development of jaw bone and expressions of serum insulin-like growth factor 1 (IGF-1) and platelet-derived growth factor BB (PDGF-BB) in rats with unilateral condylar neck fracture. Methods　The experiment began on October 25, 2019. In this study, a surgical model of unilateral condylar neck fracture was created in male SD rats. Three groups were established, including a surgical group, a surgical administration group receiving 0.9 mg/kg of compound ossotide injection daily, and a blank control group, with 24 rats in each group. The rats were observed and sacrificed after 1, 3, 5, and 9 weeks, 6 rats in each group each time, and changes in occlusal symmetry, mandibular length, mandibular ramus height, and serum levels of IGF-1 and PDGF-BB were evaluated. Analysis of variance and LSD test were used. Results　After unilateral condylar neck fracture, the mandible of the normal side developed more than that of the affected side in the surgical group, and the mandible showed deviation phenomenon. The mandible in the surgical administration group showed less deviation than that in the surgical group, and the healing of the fracture on X-ray was accelerated. Serological results showed that the levels of PDGF-BB in the surgical group, the surgical administration group, and the blank control group 1 week after surgery were (104.21±15.01) ng/L, (241.69±22.49) ng/L, and (406.47±26.88) ng/L, respectively, with statistically significant differences between two of the three groups (all P<0.001); the levels of IGF-1 5 weeks after surgery were (2 109.95±181.74) ng/L, (2 714.89±180.21) ng/L, and (2 499.27±186.04) ng/L, respectively, with statistically significant differences between two of the three groups (all P<0.05). Conclusions　Both unilateral condylar neck fracture and the use of ossotide affect the growth and development of the mandible and the contents of PDGF-BB and IGF-1 in serum of male SD rats at peak growth. There is arrested or slower mandibular development and skewed mandible on the affected side after fracture, while the healing of broken ends of the fracture will be accelerated and the degree of mandibular skewing will be decreased after injection of compound ossotide, and the changes of the two factors in serum are dynamic, with different contents at different time points.

Key words:

Mandible, Condylar neck fracture, Ossotide, IGF-1, PDGF-BB, Animal experiment

蒋骞陈毅飞陈雅秋朱志丹张嘉杰孙晓婧.

复方骨肽注射液对髁突骨折后大鼠颌骨发育的影响 [J]. 国际医药卫生导报, 2023, 29(17): 2446-2451.

Jiang Qian, Chen Yifei, Chen Yaqiu, Zhu Zhidan, Zhang Jiajie, Sun Xiaojing.

Experimental study on the effect of ossotide on the development of jaw bone in rats after condylar fracture [J]. International Medicine and Health Guidance News, 2023, 29(17): 2446-2451.

参考文献

[1] Cha S, Park G, Lee BS, et al. Retrospective clinical study of mandible fractures[J]. Maxillofac Plast Reconstr Surg. 2022 Nov 2;44(1):36. DOI: 10.1186/s40902-022- 00365-3.
[2] Srinivasan B, Balakrishna R, Sudarshan H, et al. Retrospective analysis of 162 mandibular fractures: an institutional experience[J]. Ann Maxillofac Surg, 2019, 9(1):124-128. DOI: 10.4103/ams.ams_36_18..
[3] 陈建中,杨春,刘春雷,等. 大鼠髁突软骨在髁突颈骨折和截骨致异常应力下的超微结构改变[J]. 重庆医学,2015(16):2161-2163. DOI:10.3969/j.issn.1671-8348.2015.16.001.
[4] 刘晓东,张勉,王美青. 咬合紊乱导致颞下颌关节髁突异常改建[J]. 中国实用口腔科杂志,2017,10(6):335-340. DOI:10.19538/j.kq.2017.06.004.
[5] 胡凯,周继林,刘洪臣,等. 单侧髁突骨折对下颌骨应力分布的影响[J]. 中华口腔医学杂志,1999,34(6):337. DOI:10.3760/j.issn:1002-0098.1999.06.005.
[6] 赵东强. 应力下髁突软骨中相关细胞因子的作用[J]. 国际口腔医学杂志,2011,38(4):440-442. DOI:10.3969/j.issn.1673-5749.2011.04.020.
[7] 包崇燕. 局部应用IGF-I对偏侧咀嚼大鼠髁突软骨影响的实验研究[D]. 昆明:昆明医科大学,2014.
[8] 官建中,刘亚军,王照东,等. 联合应用外源性TGF-β2及IGF-1对大鼠骨折愈合的影响[J]. 中国矫形外科杂志,2016,24(2):165-170. DOI:10.3977/j.issn.1005-8478.2016. 02.15.
[9] 魏琴,买买艾力·玉山,张继秀,等. PDGF-BB诱导兔骨髓间充质干细胞向成骨细胞分化的实验研究[J]. 新疆医科大学学报,2021,44(3):290-296. DOI:10.3639/j.issn.1009- 5551.2021.03.006.
[10] 毛利靖,叶洁瑜,梁恩瑜,等.PDGF-BB促粒细胞/巨核细胞生长及其作用机制的研究[J].中国实验血液学杂志,2017,25(2):555-561.
[11] 潘志燕,马旭亮,代福月,等. 儿童髁突骨折不同治疗方式的选择及疗效回顾性分析[J]. 中国美容医学,2021,30(11):90-94.
[12] 徐昕,褚涵文,徐菲菲,等. 髁突骨折热点问题探讨[C]//中华口腔医学会口腔颌面外科专业委员会.第十四次中国口腔颌面外科学术会议论文汇编,2018:1.
[13] 姚志涛,安玮,买买提吐逊·吐尔地. 保守治疗对儿童单侧髁突骨折患者颌骨对称性的影响[J]. 上海口腔医学,2021,30(1):77-80. DOI:10.19439/j.sjos.2021.01.015.
[14] 王玲. 注射用骨肽辅助治疗骨折愈合的效果及护理观察[J]. 药品评价,2019,16(21):35-36. DOI:10.3969/j.issn.1672-2809.2019.21.018.
[15] 张大伟. 复方骨肽注射液对胫骨骨折患者足踝功能及骨生长因子的影响[J]. 中外医疗,2021,40(28):21-24. DOI:10.16662/j.cnki.1674-0742.2021.28.021.
[16] 姜溪,陈芙蓉,任雷鸣,等. 注射用复方骨肽的药效学及其作用机制研究[J]. 现代药物与临床,2017,32(6):961-966. DOI:10.7501/j.issn.1674-5515.2017.06.001.
[17] 李嘉,单兆臣,宋燕敏,等. 髁突骨折不同治疗方式的选择及疗效分析[J]. 口腔疾病防治,2018,26(9):569-573. DOI:10.12016/j.issn.2096-1456.2018.09.005.
[18] 蔡恒星,龙星,孟庆功,等. 不同年龄段髁突骨折患者CBCT影像特点的比较研究[C]//中华口腔医学会颞下颌关节病学及牙合学专业委员会.中华口腔医学会第十五次全国颞下颌关节病学及牙合学学术研讨会论文汇编,2018:180.
[19] 刘向辉,斯方杰,孙卫革,等. 青少年髁突骨折诊治的回顾性分析[J]. 口腔颌面外科杂志,2007,17(2):165-167. DOI:10.3969/j.issn.1005-4979.2007.02.017.
[20] 顾林华,高金星,李小军. 注射用骨肽辅助治疗颌面部骨折疗效观察[J]. 浙江中西医结合杂志,2009,19(12):763-764. DOI:10.3969/j.issn.1005-4561.2009.12.024.

复方骨肽注射液对髁突骨折后大鼠颌骨发育的影响

Experimental study on the effect of ossotide on the development of jaw bone in rats after condylar fracture

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

[1]	余晴晴宋青玥柏建峰. 石蒜碱通过抑制内质网应激干预博来霉素诱导肺纤维化小鼠 [J]. 国际医药卫生导报, 2023, 29(8): 1102-1107.
[2]	陈思奇彭荣东徐嘉琪冯俊霞周晓莹张云芳苏妍妍. Drp1介导线粒体能量代谢拮抗大鼠缺血再灌注肾损伤 [J]. 国际医药卫生导报, 2023, 29(5): 663-669.
[3]	王浩坤刘成霞. 基于细胞焦亡途径探讨高良姜素对小鼠溃疡性结肠炎的保护机制 [J]. 国际医药卫生导报, 2023, 29(11): 1546-1552.