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    01 May 2022, Volume 28 Issue 9
    Scientific Research
    Professor Huang Jianling's experience in treatment of gynecological malignant tumors in period of peri-radiotherapy and chemotherapy
    Hu Xiaoxia, Liu Ran
    2022, 28(9):  1185-1189.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.001
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    Professor Huang Jianling pointed out that the occurrence and development of gynecological malignant tumors is a process of asthenia of healthy qi and sthenia of pathogenic factors. The deficiency of healthy qi, especially the deficiency of spleen and kidney qi, plays an important role in the occurrence of gynecological malignant tumors. During the period of peri-radiotherapy and chemotherapy, combined with the traditional Chinese medicine multi-way characteristic therapy, by strengthening the body resistance and eliminating evil, traditional Chinese medicine is used according to syndrome differentiation to reduce the adverse reactions caused by chemotherapy and radiotherapy and to improve the patients' immune ability to fight tumor growth.

    Application of Rosa rugosa in traditional Chinese medicine and ethnic medicines
    Cairenlamao, Qiherima, Sarangowa
    2022, 28(9):  1190-1194.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.002
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    Rosa rugosa is a deciduous shrub of the genus Rosa, and is also known as Paihuai flower and Cimei flower. It has a history of application in traditional Chinese medicine and other ethnic medicines, such as Mongolian and Uygur. This paper refers the records of Rosa rugosa in ancient documents and pharmacopoeia to compare the variant names, property theories, function indications, and clinical application of Rosa rugosa in traditional Chinese medicine and ethnic medicines. And its chemical composition and pharmacological action of modern research is summarized through the literature analysis. This paper is trying to provide references for further studies and clinical application of Rosa rugosa.
    Correlation between waist-to-height ratio and diabetic retinopathy in over-weight patients with type 2 diabetes mellitus
    Huang Ke, Shi Shousen, Zhang Yanfang
    2022, 28(9):  1195-1199.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.003
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    Objective To assess the correlation between waist-to-height ratio (WHtR) and diabetic retinopathy (DR) in over-weight patients with type 2 diabetes mellitus (T2DM). Methods A total of 620 over-weight patients with T2DM treated at Department of Endocrinology, Luoyang Central Hospital Affiliated to Zhengzhou University from November 2019 August 2020 were included for a retrospective cross-sectional study. According to whether they were complicated with DR, the patients were divided into an over-weight T2DM group and an over-weight T2DM combined with DR group. Their general data and clinical indicators were collected. The t test was used for the comparison between the two groups, and the chi-square test was used for the comparison of count data between the two groups. The correlation between WHtR and DR in the over-weight patients with T2DM were statistically analyzed. Results There were 419 cases (67.6%) in the over-weight T2DM group, including 282 males and 137 females; there were 201 cases (32.4%) in the over-weight T2DM combined with DR group, including 104 males and 97 females. Compared with the over-weight T2DM group, the over-weight T2DM combined with DR group had a higher proportion of men (P<0.001), relatively higher height (P=0.001), higher body mass index (BMI) (P=0.023), larger waist circumference (P=0.001), larger hip circumference (P=0.011), higher WHtR (P<0.001), relatively longer duration of T2DM (P<0.001), higher HbA1c (P<0.001), and a higher incidence of hypertension (P<0.001). The multivariate logistic regression analysis showed that gender (OR=0.566, 95%CI 0.331-0.969, P=0.038), height (OR=0.933,95%CI 0.913-0.952, P<0.001), waist circumference (OR=1.163, 95%CI 1.083-1.249, P<0.001), WHtR (OR=1.074, 95%CI 1.063-1.857, P=0.007), T2DM course (OR=1.056, 95%CI 1.027-1.087, P<0.001), HbA1c (OR=1.560, 95%CI 1.391-1.749, P<0.001), hypertension (OR=1.478, 95%CI 1.323-1.707, P<0.001) might be the associated risk factors with DR in the over-weight patients with T2DM. Conclusion WHtR is associated with DR in over-weight patients with T2DM.
    Effects of Acorus tatarinowii, Polygala tenuifolia, and their compatibility on ox-LDL-induced injury of human umbilical vein endothelial cells
    Chen Zhining, Liu Xiaojun, Tang Liang, Liang Weihai, Lin Guowei
    2022, 28(9):  1199-1203.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.004
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    Objective To investigate the effects of Acorus tatarinowii, Polygala tenuifolia, and their compatibility on ox-LDL-induced injury of human umbilical vein endothelial cells (HUVECs). Methods From January to August 2021, HUVECs were cultured, and were randomly divided into a blank group, a model group, and sample groups. The different concentrations (5, 10, and 20 mg/ml) of Acorus tatarinowii, Polygala tenuifolia, and their compatibility were added into the sample groups. The HUVECs proliferation, relative viability test, apoptosis, cell cycle, and expressions of apoptotic proteins Bax and Bcl-2 were detected. One-way ANOVA was applied. Results Acorus tatarinowii, Polygala tenuifolia, and their ompatibility could reduce the apoptosis of HUVECs [the blank group (0.338±0.001), the model group (4.319±0.124), the Acorus tatarinowii group (2.694±0.561), the Polygala tenuifolia group (2.278±0.914), and the Acorus tatarinowii + Polygala tenuifolia group (2.250±0.699)], and increase HUVECs cycle (S+G2)%. Both Acorus tatarinowii and Polygala tenuifolia could down-regulate the apoptotic protein Bax and inhibit the up-regulation of the apoptotic protein Bcl-2, and their compatibility was better than Acorus tatarinowii and Polygala tenuifolia. Conclusion Acorus tatarinowii, Polygala tenuifolia, and their ompatibility can inhibit ox-LDL induced HUVECs injury, and their compatibility is stronger than single Chinese medicine.
    Screening of PPARγ agonists from natural products based on computer molecular modeling
    Liu Zhiguo, Cui Wenqiang, Wei Ye, Zhu Xinyao, Yang Xue
    2022, 28(9):  1204-1208.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.005
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    Objective To screen PPARγ agonists from natural products by molecular docking. Methods This study was from January to December 2021. Based on the crystal structure of peroxisome proliferator-activated receptor gamma (PPARγ) (PDB Code: 6md4), a virtual target model was constructed. A total of 1 680 compounds in the natural product database were used as the ligands for screening, and the 6md4 pro-ligands were used as control. Firstly, the SYBYL software was used for the virtual screening of natural small molecular compounds, followed by high-precision molecular docking of the top 20 compounds, and finally the PyMol software was used to analyze the stable binding compounds. Results According to the scoring results, crash, polar, 20 small-molecule PPAR gamma agonists, such as hesperidin, and the like, were finally screened out. Among them, hesperidin could form hydrogen bonds with many amino acids, such as PPARγSer289, His323, Tyr327, and so on. Conclusion The potential PPARγ agonists were screened from natural products so as to provide basis for the discovery of new anti-diabetic lead compounds or dietary supplements.
    One case of misdiagnosed invasive fibroma on abdominal wall and literature review
    Li Nan, Cui Xiujuan, Ding Baijuan, Duan Chao, Shi Juanjuan
    2022, 28(9):  1209-1212.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.006
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    Objective To explore the diagnosis and treatment process of one misdiagnosed patient with invasive fibroma on abdominal wall, review relevant literatures, analyze the clinicopathological characteristics and diagnosis and treatment methods of invasive fibroma patients, and improve clinicians' understanding of invasive fibroma. Methods One 37-year-old female patient presented to the hospital because of a self-inflicted abdominal incision mass for 6 months. The physical examination showed that a hard mass with a size of about 5 cm×3 cm was detected at the lower left of the cesarean section scar. Preoperative diagnosis was considered as abdominal wall incision endometriosis, and abdominal wall tumor resection was performed. Results Postoperative histopathology revealed invasive fibroma, and a second operation was performed in another hospital. Conclusion For abdominal wall mass patients with a history of cesarean section, attention should be paid to the differential diagnosis of invasive fibroma on abdominal wall in clinical work to avoid secondary surgical treatment.
    Correlation between low back pain and pain score evaluated by MRI multi-quantitative sequence
    Bi Junying, Liu Liangjin, Li Shenghong, Fang Jicheng, Hu Junwu
    2022, 28(9):  1212-1217.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.007
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    Objective To analyze the correlation of the multi-quantitative sequence of magnetic resonance imaging (MRI) with low back pain and score of Oswestry Disability Index (ODI). Methods One hundred and twenty patients with low back pain who underwent lumbar MRI in Hubei No.3 People's Hospital of Jianghan University from April 2018 to October 2019 were randomly selected, including 54 males and 66 females, and they were 18-42 (32.18 ± 3.24) years old. According to the duration of pain, they divided into an acute low back pain group (45 cases; the pain duration ≤ 12 weeks) and a chronic low back pain group (75 cases; the pain > 12 weeks). The quantitative values of T1, T2, and PD of anterior annulus fibrosus, posterior annulus fibrosus, nucleus pulposus, and paravertebral muscles between L1/2 and L5/S1 were obtained. The Visual Analogue Scale (VAS) and ODI were used to evaluate the degree of pain and dysfunction. The statistical differences in the general data, VAS score, and ODI score between the two groups were analyzed by the independent-sample t test. Pearson correlation was used to analyze the correlation of the VAS and ODI scores with the clinical data and the T1, T2 and PD quantitative values of anterior and posterior annulus fibrosus, nucleus pulposus, and paravertebral muscles. Results There were no statistical differences in the general data, such as gender, age, and Pfirrmann classification, between the two groups (all P>0.05). There were no statistical differences in the scores of VAS and ODI between the two groups (both P>0.05). There were statistical differences in the T1 and T2 values of nucleus pulposus, T1 value of anterior annulus fibrosus, and T2 value of psoas major muscle between the two groups (all P<0.05). Pearson correlation analysis showed that the VAS and ODI scores were positively correlated with Pfirrmann grade, the VAS score was positively correlated with the T2 value of multifidus muscle, the ODI score was positively correlated with the body mass index and T2 value of posterior annulus fibrosus and negatively correlated with the PD, T2, and T1 values of nucleus pulposus in the acute low back pain group (all P<0.05); the VAS score was positively correlated with the PD and T1 values of posterior annulus fibrosus and the ODI score was positively correlated with the T2 value of posterior annulus fibrosus in the chronic low back pain group (r=0.414, 0.219, and 0.196; all P<0.05). Conclusions The PD, T2, and T1 values of lumbar disc and paraspinal muscles are significantly correlated with VAS and ODI scores. The values of PD, T2, and T1 play an important role in the evaluation of lumbar disc degeneration.
    Correlation between white blood cell count and metabolic syndrome in young and middle-aged male employees in steel industry in Guangzhou
    Mo Pei, Li Jiao, Zhong Yun, Liu Shiming, Wu Junyu, Gao Fang, Pei Jingxian
    2022, 28(9):  1218-1221.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.008
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    Objective To understand the association between metabolic syndrome (MS) and white blood cell count (WBC) in young and middle-aged men in steel industry in Guangzhou. Methods This was a cross-sectional study. A total of 4 241 employees who worked at 16 units of some steel group in 2009 in Guangzhou were selected by the cluster random sampling for questionnaire survey and physical examination, including 3 760 males, and they were (38.400±10.258) years old. According to the WBC, they were divided into 3 groups[WBC(3.40~5.70)×109/L:1 162 persons, (>5.70~6.90)×109/L:1 196 persons, >6.90×109/L:1 402 persons]. The correlation analysis was used to study the correlation between WBC and MS-related components. Multivariate logistic regression was used to analyze the change of MS prevalence as the WBC increased. Results The multivariate logistic regression results showed that after adjusting age, smoking, drinking, and other factors, as the WBC increased, the risk of MS increased. Compared with the lowest value of WBC, the OR values of the second and third groups were 1.88 and 2.58, respectively, with a statistical difference (P<0.05). Conclusion WBC increases in the metabolic syndrome and metabolic abnormal groups of young and middle-aged male steel workers in Guangzhou, and may be an independent risk factor for MS.
    Significance and influencing factors of serum level of ummethylation INSDNA in patients with newly diagnosed type 2 diabetes mellitus
    Wang Shaobo, Bao Liwen, Yin Liming, Deng Li, Fu Wenjin
    2022, 28(9):  1222-1226.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.009
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    Objective To investigate the significance and influencing factors of serum level of ummethylation insulin DNA (INSDNA) in patients with newly diagnosed type 2 diabetes mellitus (NT2DM) by case control study. Methods Forty-five patients with NT2DM hospitalized at Department of Endocrinology, Dongguan Houjie Hospital from October 2018 to May 2020 were selected, including 30 males and 15 females, and they were (47.0±9.6) years old. There were 23 patients with long course T2DM (LT2DM), including 15 males and 8 females, and they were (48.6±8.6) years old. There were 20 patients with normal glucose tolerance (NGT), including 13 males and 7 females, and they were (40.2±5.5) years old. The serum level of unmethylated INSDNA was detected by drop digital polymerase chain reaction (ddPCR), and the levels of human cathepsin S (Cath-S) and interleukin 6 (IL-6) by enzyme linked immunosorbent assay (ELISA). One-way ANOVA was used to analyze the difference in the unmethylated INSDNA level between the three groups, and Spearman correlation analysis was used to analyze the correlation of unmethylated INSDNA level with fasting C peptide (FC), Cath-S, and IL-6. Independent-sample t test and χ2 test were applied. Results The serum level of unmethylated INSDNA in the patients with NT2DM was significantly higher than those in the patients with NGT and the patients with LT2DM [3.01 (4.64) copies/μl vs. 0.59 (0.19) copies/μl and 1.77 (2.80) copies/μl], with statistical differences (both P<0.01). The levels of Cath-S and IL-6 in the patients with NT2DM were significantly higher than those in the patients with NGT [683.37 (283.90) nmol/L vs. 479.70 (111.30) nmol/L and 28.95 (9.99) pg/ml vs. 3.42 (6.90) pg/ml; both P<0.01]. The correlation analysis showed that the level of unmethylated INSDNA was positively correlated with the levels of FC, Cath-S, and IL-6 in the patients with NT2DM (r=0.354, P=0.017; r=0.320, P=0.032; r=0.434, P=0.003). Conclusions The serum level of unmethylated INSDNA is higher in patients with T2DM during early stage and decreases as the disease course prolongs. Serum Cath-S, IL-6, and FC may be important factors affecting the level of unmethylated INSDNA.
    Influence of graphics context propaganda and education combined with empathy communication on compliance of patients undergoing coloscopy 
    Li Hui, Gao Tingting, Dong Yong
    2022, 28(9):  1227-1230.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.010
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    Effect of continually improving hospital ward high risk drug management quality by PDCA cycle
    Zeng Shaoyan, Xie Liping, Peng Shumei, Tian Fang, Gu Meiting, Zhang Xiaolong, Pan Jihong, Zhang Lin
    2022, 28(9):  1231-1234.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.011
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    Objective To explore the effect of PDCA cycle in the high-risk drug management in hospital wards, to standardize the management of high-risk drugs, and to improve the safety of drug use. Methods A quality control circle group was organized in February 2017 at Nursing Department, Guangdong Provincial Maternity and Child Care Center. There were 1 vice-chief pharmacist, 1 chief doctor, 1 chief nurse, 1 vice-chief nurse, 4 nurses-in-charge, and 7 senior nurses in this group, with an average age of 31.25, and they averagely worked 10.5 years. From May 2017 to February 2018, the PDCA cycle management mode was applied to continually improve the hospital ward high risk drug management quality, and the effect was evaluated by the self-made questionnaire. The SPSS 15.0 software was used to analyzed the data. The data before and after the activity were compared by the paired t test. Results The scores of high risk drug catalog and cardinal management, expired period management, storage management, warning label management, shift change management, quality control management, prevention measures, supervision of use, and report of adverse events were significantly higher after than before the activity [(9.25±0.67) vs. (7.23±0.66), (9.03±0.69) vs. (7.28±0.58), (9.41±0.50) vs. (8.97±0.59), (8.91±0.78) vs. (8.25±0.62), (8.94±0.80) vs. (7.78±0.71), (9.25±0.57) vs. (8.72±0.73), (9.19±0.59) vs. (8.78±0.71), (9.22±0.56) vs. (7.47±0.62), (9.19±0.54) vs. (6.72±0.77), and (8.88±0.16) vs. (6.31±0.64)], with statistical differences (all P < 0.05). Conclusion Quality control circle activity can effectively promote the continuous improvement of the management quality of hospital ward high risk drugs, and improve the safety of drug use and the team's comprehensive management capabilities.
    Effect of quality control circle activities on reducing the rate of physical restraint in inpatients with mental illness
    Zhu Jieying, Huang Xingxiao, Chen Yanling, Zhou Huan, Mei Xiuqun
    2022, 28(9):  1235-1238.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.012
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    Objective To explore the effect of quality control circle activities on reducing the rate of physical restraint in inpatients with mental illness. Methods The inpatients with psychosis who used physical restraint in Brain Hospital Affiliated to Guangzhou Medical University from January to December 2019 were selected as a pre-intervention control group, and routine management was adopted. From January to December 2020, the hospitalized psychiatric patients with physical constraints were used as a post-intervention research group, and one-year quality control circle activities was carried out. χ2 test was used to analyze the difference in the inpatients' physical restraint rates before and after the implementation. Results After the implementation of the activities, the physical restraint rate of the psychiatric patients decreased significantly [1.99% (272/13 663) vs. 0.88% (95/10 795)], with a statistical difference (χ2=48.914, P<0.05). Conclusion Quality control circle activities can significantly reduce the physical restraint rate of inpatients with mental illness, and form a standardized effective measure, which not only ensures the patients' safety, but also improves the overall work quality of our department.
    Effect of perioperative intervention measures based on thromboelastogram for elderly patients with femoral neck fracture
    Ke Mingchi, Hu Dong, Zhu Fajun, Chen Hui, Wu Xinmiao
    2022, 28(9):  1239-1242.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.013
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    Objective To explore the effect of perioperative intervention measures based on thromboelastogram (TEG) for elderly patients with femoral neck fracture. Methods Fifty-four elderly patients with femoral neck fracture treated at Sucheng District People's Hospital from August 2020 to August 2021 were selected as the research objects. The patients were divided into a control group and an experimental group by the random number table method, with 27 cases in each group. The control group had 6 males and 11 females, and they were (73.14±2.58) years old. The experimental group had 14 males and 13 females, and they were (73.15±2.64) years old. The control group were given routine perioperative intervention measures, and the experimental group perioperative intervention measures based on TEG. The probabilities of postoperative thrombotic events, coagulation function, and TEG related indicators were compared between the two groups. The independent-sample t, paired t, and χ2 tests were applied. Results The probability of postoperative thrombotic events was 0 in the experimental group, and was 22.22% (6/27) in the control group, with a statistical difference (χ2=4.687,P=0.030). Before the intervention, there were no statistical differences in D-dimer (D-D), fibrinogen (Fib), prothrombin time (PT), and activated partial thromboplastin time (APTT) between the two groups (all P>0.05). After the intervention, the levels of D-dimer and Fib decreased and the PT and APTT prolonged in both groups, with statistical differences (all P<0.05). The levels of D-D and Fib were lower and the PT and APTT were longer in the experimental group than in the control group, with statistical differences (all P<0.05). There were no statistical differences in the TEG indicators [reaction time (R), clotting time (K), maximum amplitude (MA), and α angle] between the two groups (all P>0.05). After the intervention, the TEG indicators were better than those before the intervention (all P<0.05), and were better in the experimental group than in the control group (all P<0.05). Conclusion Perioperative intervention measures based on thromboelastogram for elderly patients with femoral neck fracture is effective and can improve their blood hypercoagulability and quality of life and prevent venous thrombosis.
    Clinical characteristics and management strategies of 2019-nCoV in pregnant women
    Tu Jiaoqin, Shi Jiaoyang, Fan Tao, Lei Qiong, Chen fengying
    2022, 28(9):  1243-1246.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.014
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    COVID-19 is a new type of highly contagious viral pneumonia. It has been identified as a zoonotic disease, and the pathogen causing the disease is similar to SARS coronavirus and Middle East respiratory syndrome coronavirus. It is highly contagious among populations, named 2019-nCoV, and poses a huge threat to global public health. With the outbreak of the 2019-nCoV epidemic, the clinical characteristics, pregnancy outcomes, and the potential risks of vertical transmission of pregnant women with COVID-19 infection have become important issues for obstetricians to consider. Viral nucleic acid testing combined with imaging tests are rapid standard diagnostic methods for new coronary pneumonia and essential to prevent subsequent secondary transmission. Mastery of 2019-nCoV disease characteristics and epidemiology is beneficial to develop effective prevention and management strategies. Project management for high-risk pregnant women, popularization of epidemic prevention related knowledge, multidisciplinary diagnosis and treatment of diagnosed patients, and standardized individual treatment are conducive to good maternal and infant outcomes.
    Qualitative research on breast cancer patients with lymphedema on their affected limbs after surgery in delaying visiting doctors
    Chen Chujun, Chen Ling, Shi Jinghua, Xu Wanzhu, Luo Zebin
    2022, 28(9):  1247-1250.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.015
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    Functional exercise compliance in patients with stroke
    Lin Jingshan, Tang Shiheng, Wang Yiyang, Li Yuxia
    2022, 28(9):  1251-1256.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.016
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    This article reviews the assessment tools, influencing factors, and intervention studies on the compliance of functional exercises in patients with stroke in order to provide references for clinical rehabilitation nursing practice of stroke patients.
    Effect applying steam cleaning machine in cleaning medical instruments provided by other companies such as files
    Wei Qiong, Chen Mengyun, Lin Xiaozhu, Zheng Yunci, Xie Man
    2022, 28(9):  1256-1258.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.017
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    Objective To evaluate the effect applying steam cleaning machine in cleaning medical instruments provided by other companies such as files. Methods Eighty sets of medical instruments received by Jieyang People's Hospital from other companies from March to May 2021 were set as an observation group, and were cleaned by the steam cleaning machine. And eighty sets of medical devices instruments received by Jieyang People's Hospital from September to November 2020 were set as a control group, and were cleaned by ultrasound and high-pressure water gun before machine cleaning. After finishing the cleaning procedure, the cleaning effects of the two groups were evaluated by the visual method and protein residue test. The comparison between groups was performed by χ2 test. Results The qualified rates detected by the visual method and protein residue test in the observation group were significantly higher than those in the control group [100.00% (80/80) vs. 90% (72/80) and 96.25% (77/80) vs. 86.25% (69/80)], with statistical differences (χ2=8.421 and 5.010; P=0.004 and 0.025). Conclusion The application of steam cleaning machine in cleaning medical instruments provided by other companies such as files can improve the cleaning effect, ensure the safety of medical instruments, and provide references for the improvement of the cleaning operation procedures of such instruments.
    Laboratory Medicine Column
    IgM detection results of antibodies against respiratory pathogens before and after COVID-19 at Songjiang District, Shanghai
    Dai Xiaoli, Yang Yixiang
    2022, 28(9):  1260-1263.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.018
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    Objective To understand the detection of respiratory pathogen immunoglobulin (Ig) M antibodies in patients with respiratory infection at Songjiang District before and after COVID-19. Methods The serum samples were collected from 5 769 patients who were 3-86 years old and were hospitalized with respiratory tract infection in Songjiang District Central Hospital from March 2019 to February 2021, including 3 335 males and 2 434 females. The indirect immunofluorescence assay was used to detect respiratory syncytial virus (RSV), adenovirus (ADV), influenza A virus (INFA), influenza B virus (INFB), parainfluenza virus (PIV), mycoplasma pneumonia (MP), chlamydia pneumoniae (CP), and legionella pneumophila (LP). The results were analyzed by Chi-square test. Results From March 2019 to February 2020, 279 of 3 579 patients with respiratory tract infection were positive in IgM antibody of respiratory tract pathogen spectrum, with a positive rate of 7.8% (279/3 579). The positive rates of INFB and INFA were 3.16% (113/3 579) and 1.06% (38/3 579), respectively. From March 2020 to February 2021, among 2 190 patients with respiratory tract infection, 284 were positive in IgM antibody of respiratory tract pathogen spectrum, and the positive rate was 12.97% (284/2 190). The positive rates of INFB and INFA were 7.21% (158/2 190) and 3.88% (85/2 190), respectively. Conclusion There are differences in the distribution of positive rates of respiratory pathogen antibody IgM at Songjiang District before and after COVID-19.

    Clinical value of UGT1A1 gene polymorphism in women with hypothyroidism during pregnancy in Bao'an area
    Yin Weiming, Lu Xiaojuan, Zhang Xuming
    2022, 28(9):  1263-1266. 
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    Objective To explore the clinical value of uridine 5′-diphospho-glucuronosyltransferase 1-1 (UGT1A1) gene polymorphism in pregnant women with hypothyroidism in Bao'an area. Methods One hundred women with hypothyroidism during pregnancy treated at Shiyan People's Hospital from January 2021 to February 2022 were selected as an observation group, and 100 healthy women with normal thyroid function taking property inspection during the same period were selected as a control group. The fasting venous blood of all the subjects was taken for RNA extraction and sequencing. The observation group were routinely treated with levothyroxine tablets for 4 weeks. The serum levels of thyroid stimulating hormone (TSH) and thyroid peroxidase antibody (TPOAb) were detected. The χ2, paired t, or F tests were applied. Results In the observation group, there were 73 cases of UGT1A1*6 wild type, 23 cases of heterozygous mutation type (G/A), 4 cases of homozygous mutation type (A/A), 70 cases of UGT1A1*28 wild type, 27 cases of G/A type, and 3 cases of A/A type; in the control group, there were 69 cases of UGT1A1*6 wild type, 26 cases of G/A type, 5 cases of A/A type, 72 cases of UGT1A1*28 wild type, 26 cases of G/A type, and 2 cases of A/A type; there were no statistical differences in UGT1A1*6 and UGT1A1*28 gene polymorphisms between the observation group and the control group (both P>0.05). Before the treatment, there were no statistical differences in TSH and TPOAb levels between the patients with different UGT1A1*6 and UGT1A1*28 genotypes (all P>0.05); after the treatment, the TSH and TPOAb levels of the patients of UGT1A1*6 and UGT1A1*28 wild types were the highest, those of the patients of UGT1A1*6 G/A type were the second, and the TSH level of the patients of UGT1A1*6 A/A type was the lowest, with statistical differences (all P<0.05). Conclusions The UGT1A1 gene polymorphism in women with hypothyroidism during pregnancy is similar to that in healthy pregnant women. The levels of TSH and TPOAb in patients with UGT1A1*6 and UGT1A1*28 homozygous mutant hypothyroidisms during pregnancy after clinical treatment are significantly reduced. Clinically, it should consider to give an appropriate dose of drug treatment according to the UGT1A1 genotypes.

    Analysis of semen quality and sex hormone levels in 131 obese and infertile male patients
    Liu Anna, Wang Chen
    2022, 28(9):  1267-1269.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.020
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    Objective To analyze the influence of obesity on semen quality and sex hormone levels in infertile males. Methods A total of 131 obese and infertile male patients treated in Reproductive Medicine Center, Hospital of Seventy-third Group, Land Army from January 2018 to May 2021 were recruited into the obesity group, and they were 22-47 years old. During the same period, 160 healthy males receiving physical examination at Hospital of Seventy-third Group, Land Army were recruited into the healthy control group, and they were 25-44 years old. The semen qualities and sex hormone levels were compared between these two groups. The independent-sample t test was used for the statistical analysis. Results The sperm density, motility, forward movement rate in the obesity group were significantly lower than those in the control group [(47.43±21.19)×106 /ml vs. (65.09±28.58)×106/ml, (50.76±13.63)% vs. (63.39±18.30)%, and (30.94±13.76)% vs. (43.52±17.12)%; all P<0.05]. There were no statistical differences in the semen volume and pH value between these two groups (both P>0.05). The levels of serum testosterone (T) and luteinizing hormone (LH) were significantly lower and the serum estradiol (E2) was significantly higher in the obesity group than in the control group [(3.82±1.20) μg/L vs. (5.78±1.85) μg/L, (4.23±1.37) mIU/ml vs. (5.93±1.16) mIU/ml, and (36.11±11.52) ng/L vs. (27.26±13.14) ng/L; all P<0.05]. There was no statistical difference in the FSH level between these two groups (P>0.05). Conclusion Obesity can affect semen quality and sex hormone levels, and induce male infertility.
    Treatises
    Expression and function of ATP6V1C1 in hepatocellular carcinoma
    Jiang Min, Zhao Yujun
    2022, 28(9):  1270-1276.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.021
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    Objective To investigate the expression and function of ATP6V1C1 in hepatocellular carcinoma (HCC). Methods This was a basic study. The sequencing data from the Cancer Genome Atlas database GEO (Gene Expression Omnibus) were used to identify the differentially expressed genes in HCC (P<0.01, |logFc|≥1), and the data from the TCGA (The Cancer Genome Atlas) online data UALCAN and Timer were used to analyze the expressions and function of ATP6V1C1 in different stages of HCC. The TCGA database contained 121 female patients and 281 male patients, and they were 16-81 years old. KEGG signaling was used to understand the effect of ATP6V1C1 on the relevant pathways. The pcDNA3.1(+)-ATP6V1C1 vector was constructed, and the siRNA interference fragments were purchased to study the effect of ATP6V1C1 on hepatocellular carcinoma in vitro. The independent-sample t test was used. Results Bioinformatics analysis suggested that ATP6V1C1 was upregulated in a variety of cancers (P<0.05); ATP6V1C1 expression correlated with HCC stage; and in patients with HCC, the patients with upregulated ATP6V1C1 expression had a worse prognosis than those with downregulation (P<0.05). The overexpression of ATP6V1C1 significantly promoted the invasion, migration, proliferation, and clone-forming ability of HCC cells, while interference with ATP6V1C1 inhibited the invasion, migration, proliferation, and clone-forming ability of HCC cells. Conclusions ATP6V1C1 expression in HCC correlates with prognosis. This study provides a new possible diagnostic and therapeutic target for HCC.

    Application of laparoscopic ultrasound in laparoscopic resection of liver cancer and its influence on patients

    Zhang Guijiang, Sun Bin
    2022, 28(9):  1277-1282.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.022
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    Objective To explore the application of laparoscopic ultrasound in laparoscopic resection of liver cancer and its influence on patients' margins, tumor apoptosis-related molecules, and prognosis. Methods Seventy-nine patients with small hepatocellular carcinoma treated at People's Hospital of Lanshan District from February 2016 to June 2018 were selected, including 59 males and 20 females, with an age of (53.57±10.16). They were divided into a laparoscopic ultrasound group (40 cases) and a conventional group (39 cases) according to different treatment plans. The conventional group underwent conventional laparoscopic resection of liver cancer, and the laparoscopic ultrasound group were surgically treated under laparoscopic ultrasonography. The operation and incision margins, complications, postoperative 3-year survival rates, expressions of tumor apoptosis-related molecules [apoptosis-related factors (Fas), apoptosis-related factor ligands (Fas), and FasL), and caspase-3 (Caspase-3)], and levels of serum tumor markers [alpha-fetoprotein (AFP), glypican 3 (GPC3), and carcinoembryonic antigen (CEA)] and liver function indicators [aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin (TBIL)] were compared between these two groups. The measurement data were subjected to t test. MANOVA of repeated measuring was used between different time points and groups. The χ2 test was used for the enumeration data. Results The intraoperative bleeding volume was lower, the postoperative ambulation time and hospital stay were shorter, and the average and shortest distances from the circumferential incision edge were longer in the laparoscopic ultrasound group than in the conventional group, with statistical differences (all P<0.05). Two and four weeks after the operation, the expressions of Fas [(129.42±14.68) vs. (112.85±12.79) and (142.95±16.74) vs. (125.47±14.96); F=17.185, P<0.001] and Caspase-3 mRNA [(137.30±17.08) vs. (121.16±13.71) and (151.24±18.23) vs. (132.09±16.54); F=14.769, P<0.001] in the tumor tissue were higher and the expressions of FasL mRNA [(74.85±6.73) vs. (86.36±7.52) and (65.68±5.25) vs. (79.09±6.16); F=19.059, P<0.001] were lower in the laparoscopic ultrasound group than in the conventional group (all P<0.05). The incidence of postoperative complications was 10.00% (4/40) in the laparoscopic ultrasound group, and was 28.21% (11/39) in the conventional group (χ2=4.255, P=0.039). The 3-year overall survival rate after the operation and the tumor-free survival rate were 77.50% (31/40) and 42.50% (17/40) in the laparoscopic ultrasonography group, and were 66.67% (26/39) and 35.90% (14/39) in the conventional group, with no statistical differences (χ2=1.153 and 0.361, P=0.283 and 0.548). Conclusion The application of laparoscopic ultrasound in patients undergoing laparoscopic resection of liver cancer can ensure the circumferential margins, reduce surgical trauma, regulate the expression of tumor apoptosis-related molecules, decrease tumor malignancy, improve liver function, reduce complications, and promote the patients' recovery.
    Application of dynamic intracranial pressure monitoring in suboccipital decompressive craniectomy for space-occupying cerebellar infarction
    Zhao Duanyun, Zhang Haibing, Li Guanghong, Wang Hongbo
    2022, 28(9):  1283-1287.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.023
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    Objective To explore the application of dynamic intracranial pressure (ICP) monitoring in suboccipital decompressive craniectomy (SDC) for space-occupying cerebellar infarction, and investigate the ICP changes and their impact on the patients' prognosis. Methods Twenty-two patients with space-occupying cerebellar infarction treated with SDC and dynamic ICP monitoring in Heze Municipal Hospital from October 2016 to October 2020 were retrospectively analyzed, including 11 males and 11 females, and they were 28-79 (56.4±13.5) years old. When the ICP monitoring probe was put in, before decompressive craniectomy and after the cerebrospinal fluid was released, and when the surgery was over, the ICP's were measured, and recorded as ICP1, ICP2, and ICP3. The difference between ICP2 and ICP3 was recorded as ΔICP. After the surgery, the ICP was continuously and dynamically monitored, and the ICP change and its relation with prognosis were analyzed. Independent-sample t test, paired t test, and Pearson correlation analysis were applied. Results The ICP1, ICP2, ICP3, and peak ICP were (22.3±4.3), (20.3±3.9), (15.7±3.8) and (19.4±5.2) mmHg (1 mmHg=0.133 kPa), with a statistical difference between ICP2 and ICP3 (P<0.01), and the ΔICP was (4.6±1.9) mmHg. The craniectomy area was (22.5±1.6) cm2, and the ΔICP had no correlation with it (P=0.17). By the end of the follow-up period, 18 patients survived, and 4 patients died. The score of Glasgow Outcome Scale (GOS) in the patients who survived was (3.9±1.5), and 16 patients had high survival quality, and their scores of GOS ≥ 4. The peak ICP and cumulative time for postoperative ICP to exceed the threshold (CTET) had impact on the patients' prognosis (r=0.31, P<0.01). Conclusion ICP monitoring is valuable for SDC in the treatment of space-occupying cerebellar infarction and may predict the patients' prognosis.
    Clinical Research

    Effect of nadroparin calcium combined with aspirin in treatment of recurrent spontaneous abortion and its influence on patients' serum levels of TSP-1 and sflt-1

    Xin Xiu
    2022, 28(9):  1288-1292.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.024
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    Objective To investigate the effect of nadroparin calcium combined with aspirin in the treatment of recurrent spontaneous abortion (RSA) and its effect on the levels of serum thrombospondin-1 (TSP-1) and soluble fms-like tyrosine kinase-1 (sflt-1). Methods A total of 112 patients with RSA admitted to Jiamusi Maternal and Child Health Care Hospital from January 2019 to January 2020 were selected as the observation objects, and were divided into a control group and an observation group according to the random number table method, with 56 cases in each group. The control group were (29.36±2.82) years old, had (4.79±0.45) times abortion, and their gestational age was (7.64±0.75) weeks. The observation group were (29.29±2.80) years old, had (4.77±0.43) times abortion, and their gestational age was (7.68±0.77) weeks. The control group were treated with aspirin, and the observation group with aspirin and nadroparin calcium. The four blood coagulation items [prothrombin time (PT), thrombin time (TT), activated partial thromboplatin time (APTT), and fibrinogen (FIB)], serum levels of TSP-1 and sflt-1, adverse reactions, and pregnancy outcomes were compared between the two groups. The measurement data were analyzed by t test, and the enumeration data by χ2 test. Results After the treatment, the PT, TT, and APTT were longer and the FIB level was lower in the observation group than in the control group [(12.46±1.02) s vs. (11.63±1.05) s, (14.40±1.17) s vs. (13.25±1.16) s, (29.67±2.12) s vs. (26.92±2.19) s, and (3.56±0.34) g/L vs. (4.02±0.35) g/L], with statistical differences between the two groups (all P<0.05). After the treatment, the serum levels of TSP-1 and sflt-1 in the observation group were lower than those in the control group [(0.83±0.07) μg/L vs. (1.17±0.09) μg/L and (7 512.06±731.65) μg/L vs. (9 050.79±722.10) μg/L], with statistical differences between the two groups (t=33.315 and 11.201, both P<0.001). The incidence of adverse reactions was 12.50% (7/56) in the observation group, and was 5.36% (3/56) in the control group, with no statistical difference between the two groups (P>0.05). The success rate of miscarriage protection was 83.93% (47/56) in the observation group, and was 66.07% (37/56) in the control group, with a statistical difference between the two groups (χ2=4.719, P=0.030). Conclusion Natroparin calcium in the treatment of recurrent abortion can improve the patients' coagulation function, reduce the serum levels of TSP-1 and sflt-1 and the abortion rate, and improve the success rate of miscarriage protection.
    Effect of three-dimensional gait guided pelvic control training for patients with spastic cerebral palsy
    Liu Zhanhao, Feng Chongrui, Lu Fahua, Guo Yongliang, Liu Churong
    2022, 28(9):  1292-1296.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.025
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    Objective To study the effect of three-dimensional gait guided pelvic control training for patients with spastic cerebral palsy. Methods Forty-eight patients with spastic cerebral palsy treated at Guangdong 999 Brain Hospital from May 2019 to September 2020 were randomly divided into a control group and an observation group, with 24 cases in each group. There were 13 males and 11 females in the control group, and they were (44.75±13.34) years old. There were 14 males and 10 females in the observation group, and they were (45.38±13.41) years old. The control group did routine rehabilitation training, and the observation group did pelvic control training under the guidance of three-dimensional gait on the basis of control group. Before and after the treatment, the scores of 3D gait parameters, Functional Ambyiation Category Scale (FAC), and Berg Balance Scale (BBS) of the two groups were recorded and analyzed. ANOVA was used for the comparison between these two groups. Results There were no statistical differences in the left and right step sizes, peak values of hip joint extension of left and right lower limbs, peak torques of hip joint of left and right lower limbs, low peak values of hip joint torque of left and right lower limbs, and scores of FAC and BBS between the two groups before the treatment (all P>0.05). After the treatment, there were statistical differences in the left and right step sizes [(0.545±0.08) m vs. (0.602±0.082) m, F=6.185, P=0.017; (0.587±0.115) m vs. (0.664±0.092) m, F=6.525, P=0.014], peak values of hip joint extension of left and right lower limbs [(6.87±2.17) vs. (8.92±2.53), F=8.972, P=0.004; (6.79±2.36) vs. (11.00±2.45), F=36.757, P<0.001], peak torques of hip joint of left and right lower limbs [(0.427±0.037) vs. (0.508±0.066), F=27.921, P<0.001; (0.605±0.09) vs. (0.767±0.063), F=39.422, P<0.001], low peak values of hip joint torque of left and right lower limbs [(0.402±0.040) vs. (0.455±0.068), F=10.616, P=0.002; (0.442±0.037) vs. (0.497±0.063), F=13.565, P=0.001], and scores of FAC [(2.75±0.79) vs. (3.75±0.94), F=15.771, P<0.001] and BBS [(48.90±3.74) vs. (53.00±2.85), F=17.722, P<0.001] between the control group and the observation group. Conclusion Three-dimensional gait guided pelvic control training can improve the gait and lower limb movement function of patients with spastic cerebral palsy.

    Clinical study of cardiac function and intracranial arterial hemodynamics in patients with acute cerebral infarction

    Tan Yu, Zhang Zhaoting, Liu Jianhua, Feng Shouqin, Li Wei, Chen Jiechun
    2022, 28(9):  1296-1301.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.026
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    Objective To study the changes of cardiac function and intracranial arterial hemodynamics in patients with acute cerebral infarction (ACI) by ultrasonography. Methods Seventy-nine patients with ACI treated at Department of Neurology, Lianyungang Second People's Hospital from January to December 2019 were selected as an ACI group, including 50 males and 29 females, and they were 42-87 (66.27±9.89) years old. Thirty-three healthy examinees during the same period were selected as a control group, including 19 males and 14 females, and they were (64.15±10.11) years old. Both groups underwent cardiac color Doppler ultrasonography to obtain cardiac structure and function indicators, arterial ultrasonography to obtain arterial inner diameter, blood flow velocity, resistance index, and arterial intima-media thickness, and transcranial Doppler ultrasonography to obtain intracranial artery blood flow rate. The measurement data were compared between these two groups by the independent-sample t test, and the rates by χ2 test. Results (1) The left atrial diameter, right atrial diameter, left ventricular end-diastolic diameter, and pulmonary artery pressure were (39.40±3.13) mm, (50.26±3.48) mm, (23.93±1.89) mm, and (38.11±3.37) mmHg (1 mmHg=0.133 kPa) in the ACI group, and were (38.00±3.32) mm, (48.82±2.51) mm, (22.89±1.23) mm, and (36.08±2.35) mmHg in the control group, with statistical differences (t=2.007, 2.276, 2.677, and 2.907; P=0.048, 0.026, 0.009, and 0.005). (2) The left and right common carotid artery end-diastolic blood flow velocities (Vd), left internal carotid artery Vd, and right internal carotid artery peak systolic flow velocity (Vs) in the ACI group were lower than those in the control group, with statistical differences (all P<0.05). The resistance indexes of the left and right common carotid arteries in the ACI group were higher than those in the control group, with statistical differences (both P<0.05). End diastolic velocity difference of bilateral vertebral arteries in ACI group(ΔVd) was significantly higher than that in the control group (P<0.05). (3) The Vd of the right anterior cerebral artery in the ACI group was higher than that in the control group, with a statistical difference (P<0.05). The ΔVd's of anterior cerebral arteries, middle cerebral arteries, and vertebral arteries on both sides in the ACI group were higher than those in the control group, with statistical differences (all P<0.05). Conclusion Patients with ACI can improve their brain blood supply adjusting heart structure and intra-and extra-cranial arterial blood flow velocity.
    Case Report
    A case of massive hemorrhage caused by primary cervical endometriosis cyst misdiagnosed as cervical cancer
    Huang Jinxia, Zhang Renmin, Sun Aichun, Wang Jingjing
    2022, 28(9):  1302-1304.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.027
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    Cervical endometriosis cyst is a kind of clinical pathology type of endometriosis; its incidence is low; its clinical manifestations lack specificity; and it is easy to miss diagnosis and be misdiagnosed. Here we report a case of primary cervical endometriosis cyst rupture misdiagnosed as cervical cancer with massive hemorrhage as the first presentation and review the literatures to improve the understanding of cervical endometriosis cyst.
    Two cases of severe adverse reactions caused by somatostatin for injection
    Cui Hongxia, Zhao Zhanwei
    2022, 28(9):  1305-1306.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.028
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    Somatostatin for injection is widely used in clinic, and its common adverse reactions include sickness, vomiting, diarrhea, lip numbness, allergy, arrhythmia, and so on. In this paper, two cases of drug adverse reactions induced by somatostatin, myocardial ischemia and severe hypoglycemia, were reported, so as to provide references for clinical application.

    One case of gingival metastasis of gastric cancer
    Wang Xiaoxia, Cong Peifu, Wang Ming
    2022, 28(9):  1307-1308.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.029
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    Nursing Research
    Effect of evidence-based nursing of urokinase in dredging blood coagulation blockage of deep venous catheter
    Cen Ruixian, Huang Xuehuan, Chen Zhihong, Xu Yuhua, Cai Yuqing, Feng Yudi, Wang Caizhu, Chen Huihong, Huang Yunhong, Zhu Miyi, Liu Yanyi, Zhong Jiezhen, Wu Quan
    2022, 28(9):  1309-1312.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.030
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    Objective To observe the methods and effect of evidence-based nursing in urokinase to relieve hemagglutinative occlusion of deep venous catheter. Methods A total of 720 patients who received various deep venous catheter treatments in Guangzhou Chest Hospital between January 2019 and May 2021 were selected as the research objects. Among them, 62 patients had peripherally inserted central catheterization (PICC) and central vein catheter (CVC) hemagglutinative occlusions, including 37 males and 25 females, and they were 22-73 (41.53±4.10) years old; according to the research plan, 31 cases used three-way switch negative pressure technology to open the tubes, and 31 cases used 2 ml syringe negative pressure technology to open the tubes; urokinase was used to dredge the catheters and evidence-based nursing intervention was implemented in both groups. All the data were calculated using XLS, and SPSS 18.0 software was used to summarize and analyze the catheter recanalization rate, coagulation function, and platelet changes of the 62 patients. Results After the implementation of evidence-based nursing intervention, the recanalization rate of the three-way switch negative pressure dredging method reached 83.87% (26/31), and the recanalization rate of the negative pressure dredging method of 2 ml syringe reached 100.00% (31/31), with a statistical difference (χ2=33.055, P<0.05). Among the coagulation function indicators, the D2 aggregate was normal in 9 cases and high in 53 cases; the fibrinogen was normal in 12 cases and high in 50 cases; the platelet test was normal in 50 cases, high in 9 cases, and low in 3 cases. It did not affect the clinical treatment and catheter function, and did not cause catheter damage. Conclusion Long-term clinical practice studies have confirmed that the implementation of evidence-based nursing can significantly increase the recanalization rate of PICC, CVC, and other deep venous catheters and ensure the smooth implementation of clinical treatments, so it is worth being clinically generalized.
    Application of oxygenation atomization combined with high frequency vibration expectoration in airway management after tracheotomy
    Lin Lirong, Liu Gang
    2022, 28(9):  1313-1317.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.031
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    Objective To investigate the application value of oxygenation atomization combined with high-frequency vibration sputum extraction in airway management after tracheotomy and its effect on lung function. Methods The clinical data of 69 patients who underwent tracheotomy for various reasons in Guangzhou Xinhai Hospital and Guangdong First Invalides Hospital from August 2020 to August 2021 were retrospectively analyzed, including 41 males and 28 females, and they were 31-81 years old. They were randomly divided into an oxygen atomization combined with high frequency vibration expectoration drainage group (38 cases; experimental group) and a conventional sputum suction group (31 cases, control group). According to airway stranded sputum volume, timely sputum suction was carried out. The nature sputum in within 1 week, callus, the amounts of expectoration drainage, lung infection symptoms, sputum culture, blood oxygen activities, and lung function indicators in both groups were observed and compared. The count data were expressed as cases (%), and compared between the two groups by χ2 test. The measurement data were expressed as (x±s), and were compared between these two groups by independent-sample t test. Results The 38 patients in the observation group had taken oxygenation atomization combined with high frequency vibration expectoration 462 times within one week, and 7 cases did sputum culture; the 31 patients in the control group had underwent sputum suction 1 316 times, and 13 cases did sputum culture. The sputum scab rate, sputum purulence rate, expectoration frequency, sputum volume per time, incidence of lung infection, and positive rate of sputum culture were 27.7% (128/462), 21.8% (101/462), (11.0±3.4), (26.0±5.5) ml, 18.4% (7/38), and 42.9% (3/7) in the experimental group, and were 47.6% (627/1 316), 44.7% (589/1 316), (33.0±6.8), (15.0±3.5) ml, 41.9% (13/31), and 76.9% (10/13) in the control group, with statistical differences (P=0.021, 0.016, 0.015, 0.031, 0.017, and 0.036). The rate of conscious patients completing lung function exercise was 86.8% (33/38) in the experimental group, and was 38.7% (12/31) in the control group, with a statistical difference (P=0.038). The sputum volume records showed that the proportion of total sputum volume within 3 days to the total sputum volume in 1 week was 67.8% (8 200/12 100) in the experimental group, and was 51.8% (10 200/19 700) in the control group, with a statistical differences (P=0.452). One week later, the heart rate, respiration, arterial oxygen saturation, partial pressure of oxygen, and lung function in the experimental group were better than those in the control group, but there no statistical differences (all P> 0.05). No one stopped the treatment because of obvious palpitation, shortness of breath, and blood pressure fluctuation. Conclusions Oxygenation atomization combined with high-frequency vibration expectoration can effectively dilute sputum, prevent sputum scab, and promote sputum excretion. Compared with conventional expectoration, it can significantly reduce the frequency of expectoration and the incidence of lung infection and maximize the coordination with pulmonary function rehabilitation.
    Feedforward-controlled enteral nutrition support combined with daily arousal for patients undergoing mechanical ventilation
    Pan Haiping, He Ping
    2022, 28(9):  1317-1320.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.032
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    Objective To investigate the effect of feedforward-controlled enteral nutrition support combined with daily arousal for patients undergoing mechanical ventilation. Methods From September 2019 to September 2020, 80 patients treated by mechanical ventilation in Wuxi Second People's Hospital were selected, and were divided into a control group and an experimental group by the random number table method, with 40 cases in each group. The control group had 26 males and 14 females, and were (46.35±2.37) years old. The experimental group had 28 males and 12 females, and were (48.26±3.16) years old. Routine nursing intervention was given to the control group, and the experimental group was given enteral nutrition support based on feedforward control and daily arousal. The nutritional status, the degrees of enteral nutrition tolerance, sedation treatment times, mechanical ventilation times, and ICU hospitalization times were compared between the two groups. Independent-Sample t and χ2 tests were applied. Results The levels of albumin, prealbumin, and hemoglobin were (38.97±7.16) g/L, (258.57±23.48) mg/L, and (134.67±14.37) g/L in the experimental group, which were significantly higher than those in the control group (all P<0.05). The incidence of poor enteral nutrition intolerance in the experimental group was significantly lower than that in the control group [2.5% (1/40) vs. 27.5% (11/40); χ2=9.804, P=0.002]. The sedation time, mechanical ventilation time, and ICU stay in the experimental group were (4.68±2.67) days, (6.16±3.49) days, and (6.94±4.67) days, which were significantly less than those in the control group (all P<0.05). Conclusion Feedforward controlled enteral nutrition support combined with daily arousal for patients taking mechanical ventilation can ensure adequate nutrition, reduce the occurrence of delirium, and promote their recovery.

    Summary
    Regulation of calcium-regulated signaling pathways in endometrial histopathology
    Chen Danwei
    2022, 28(9):  1321-1325.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.033
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    Signaling pathways play crucial roles in various physiological functions, and calcium is an important element in regulating cell signal transduction. Calcium-regulated signaling pathways mainly rely on cellular calcium channels and calcium-modulating proteins. Calcium-related regulatory molecules are expressed in the endometrium and dynamically regulate endometrial function in response to changes in calcium signals. Abnormal calcium-regulated signaling pathways have also been shown to be associated with a variety of endometrial-related benign and malignant tumors. The main purpose of this review is to summarize the research progress on the effects of calcium-regulatory signaling in endometrium on endometrial-related physiological and pathological changes. This paper systematically describes the complex calcium regulation in female endometrial-related diseases, and has guiding significance for clinical treatment.

    Treatment strategies for shoulder-hand syndrome after stroke
    Pei Mengge, Li Guqiang
    2022, 28(9):  1326-1329.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.034
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    Stroke has become the first cause of death in China, with high rates of morbidity, mortality, and disability. Stroke patients are often complicated with a variety of complications. Shoulder-hand syndrome is one of the most common complications. Shoulder-hand syndrome after stroke does not only cause swelling and pain and other adverse effects in patients, and also causes limb dysfunction and even deformity. With the continuous development of medical technology, the reports of shoulder-hand syndrome after stroke at home and abroad are increasing, but it is lack of consistency and integrity. The treatment for shoulder-hand syndrome after stroke reported in literatures are reviewed in this paper, so as to provide relevant references for the clinical treatment of patients with shoulder-hand syndrome after stroke.
    Research progress of osteoporotic spinal fracture 
    Meng Yonghui
    2022, 28(9):  1330-1332.  DOI: 10.3760/cma.j.issn.1007-1245.2022.09.035
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    With the steady development of society, China's aging is increasingly serious, and people pay more and more attention to their living standard, and the cognitive degree of medical knowledge is getting higher and higher, prompting the public to pay more and more attention to osteoporosis. The most common complication of osteoporosis is spinal compression fractures. According to relevant data statistics, the number of osteoporosis patients in China had grown to 114 million in 2010, with a huge number of 750 000 people increasing every year, and it will increase to 151 003 million in 2025 at this rate. Osteoporosis spinal fracture is the main cause of disability in the elderly population. Therefore, it has serious impact on the quality of life of such people and is also a social public problem. It is difficult to clinically treat this disease. According to their conditions, usually they are intervened by these measures, such as relieving pain, increasing bone mineral density, stabilization of vertebral body, etc. There were two main treatment options: surgical treatment and conservative treatment. Different treatment options have different curative effects and complications. Based on the above background, the author reviews the research progress of osteoporotic spinal fracture, so as to provide corresponding treatment references in clinic and improve the cured rate.