Abstract:

Objective　To investigate the value of serum interleukin-33 (IL-33) level in the diagnosis of bacterial, fungal, and viral enteritis in children. Methods　A total of 72 children with bacterial enteritis [38 boys and 34 girls, aged (9.6±3.2) years], 48 children with fungal enteritis [26 boys and 22 girls, aged (10.7±3.8) years], and 67 children with viral enteritis [33 boys and 34 girls, aged (10.3±3.6) years] admitted to Department of Gastroenterology, Guangdong Provincial Clinical Research Center for Child Health from June 2022 to June 2023 were selected. Another 60 cases of healthy volunteers were selected as the control group, including 30 boys and 30 girls, aged (10.9±4.2) years. The enzyme-linked immunosorbent assay (ELISA) kits were applied to monitor the IL-33 level in the serum of all the children. The diagnostic value of serum IL-33 in children with bacterial, fungal, and viral enteritis was evaluated using the receiver operating characteristic curve (ROC). Independent sample t test, ANOVA, and χ² test were used. Results　The serum IL-33 level was (72.03±32.25) ng/L in the healthy controls, (129.01±50.79) ng/L in the bacterial enteritis group, (100.47±45.65) ng/L in the fungal enteritis group, and (100.16±48.58) ng/L in the viral enteritis group. The levels of serum IL-33 in the bacterial enteritis group, the fungal enteritis group, and the viral enteritis group were higher than that in the healthy controls (t=7.516, 3.788, and 3.797, all P<0.001), and the levels of serum IL-33 in the bacterial enteritis group and the viral enteritis group were lower than that in the bacterial enteritis group (t=3.138 and 3.417, P=0.002 and 0.001), but there was no statistically significant difference between the fungal enteritis group and the viral enteritis group (t=0.034, P=0.973). The areas under the curve of IL-33 in the diagnosis of bacterial, fungal, and viral enteritis in children were 0.826 9, 0.669 8, and 0.669 9, respectively. Conclusion　IL-33 has a high diagnostic value for bacterial, fungal, and viral enteritis in children, especially in bacterial enteritis.

Key words:

IL-33, Children, Bacterial enteritis, Fungal enteritis, Viral enteritis, Diagnosis

摘要：

目的　探究血清白细胞介素-33（IL-33）水平在诊断儿童细菌性、真菌性和病毒性肠炎中的价值。方法　选取广州市妇女儿童医疗中心消化科2022年6月至2023年6月收治的187例细菌性肠炎、真菌性肠炎及病毒性肠炎患儿进行回顾性分析。72例细菌性肠炎患儿中男38例，女34例，年龄（9.6±3.2）岁；48例真菌性肠炎患儿中男26例，女22例，年龄（10.7±3.8）岁；67例病毒性肠炎患儿中男33例，女34例，年龄（10.3±3.6）岁。另选取60例健康志愿者作为对照，其中男30例，女30例，年龄（10.9±4.2）岁。采用酶联免疫吸附试验（ELISA）试剂盒检测所有患儿血清中的IL-33水平。采用受试者工作特征曲线（ROC）评价血清IL-33对儿童细菌性、真菌性和病毒性肠炎的诊断价值。采用独立样本t检验、方差分析、χ²检验。结果　健康志愿者组IL-33水平为（72.03±32.25）ng/L，细菌性肠炎组为（129.01±50.79）ng/L，真菌性肠炎组为（100.47±45.65）ng/L，病毒性肠炎组为（100.16±48.58）ng/L，细菌性肠炎组、真菌性肠炎组、病毒性肠炎组IL-33水平均高于健康志愿者组（t=7.516、3.788、3.797，均P<0.001），真菌性肠炎组和病毒性肠炎组IL-33水平均低于细菌性肠炎组（t=3.138、3.417，P=0.002、0.001），真菌性肠炎组与病毒性肠炎组比较差异无统计学意义（t=0.034，P=0.973）。IL-33诊断儿童细菌性、真菌性和病毒性肠炎的曲线下面积分别为0.826 9、0.669 8和0.669 9。结论　IL-33对儿童细菌性、真菌性和病毒性肠炎具有较高的诊断价值，尤其是儿童细菌性肠炎患者。

关键词:

白细胞介素-33, 儿童, 细菌性肠炎, 真菌性肠炎, 病毒性肠炎, 诊断