关键词:

肥胖, 儿童, 补体C1q/肿瘤坏死因子相关蛋白3, 补体C1q/肿瘤坏死因子相关蛋白9, 糖脂代谢, 胰岛素抵抗

Abstract:

Objective　To investigate the correlations between serum complement C1q/tumor necrosis factor-related protein (CTRP)3 and CTRP9 levels and glucose and lipid metabolism and insulin resistance in obese children in primary and secondary schools. Methods　A total of 80 obese children in primary and secondary schools, aged 8-14 years, who were treated in Zhumadian TCM Hospital from March 2022 to September 2023, were selected as the obese group. During the same period, 76 overweight children aged 8-14 years in primary and secondary schools were selected as the overweight group, 82 normal weight children aged 8-14 years were selected as the control group. The general data (gender, age, systolic blood pressure, diastolic blood pressure, waist circumference, hip circumference, etc.) of the children in the three groups were collected. The fasting insulin (FINS) level was detected by chemiluminescence immunoassay, the fasting blood glucose (FBG) level was detected by glucose oxidase assay, and the homeostasis model assessment insulin resistance (HOMA-IR) was calculated. The levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), and high density lipoprotein cholesterol (HDL-C) were measured by automatic biochemical analyzer. Serum CTRP3 and CTRP9 levels were detected by enzyme-linked immunosorbent assay (ELISA). Pearson correlation analysis was used to analyze the correlations between serum CTRP3 and CTRP9 levels and blood glucose, blood lipids, and insulin resistance in obese children. Multivariate logistic regression analysis was used to analyze the influencing factors of childhood obesity. One-way analysis of variance, SNK-q test, and χ² test were used. Results　The levels of waist circumference, hip circumference, TC, TG, LDL-C, FINS, FBG, and HOMA-IR in the obese group were higher than those in the control group and the overweight group, and those in the overweight group were higher than those in the control group (all P<0.05). The HDL-C in the obese group was lower than that in the control group and the overweight group, and that in the overweight group was lower than that in the control group (all P<0.05). Serum CTRP3 [(190.36±60.22) μg/L] and CTRP9 [(101.15±26.49) μg/L] levels in the obese group were lower than those in the control group [(304.83±72.51) μg/L and (152.87±30.96) μg/L] and the overweight group [(236.75±64.71) μg/L and (124.13±27.89) μg/L]; those in the overweight group were lower than those in the control group (all P<0.05). There was a positive correlation between CTRP3 and CTRP9 in serum of obese children (P<0.05). Both were positively correlated with HDL-C (both P<0.05), and were negatively correlated with TC, TG, LDL-C, FINS, FBG, and HOMA-IR (all P<0.05). FINS was a risk factor for obesity in primary and secondary school children (P<0.05), and CTRP3 and CTRP9 were protective factors for obesity in primary and secondary school children (both P<0.05). Conclusion　The decrease of serum CTRP3 and CTRP9 levels is closely associated with obesity in primary and secondary school children, which can reflect the disorder of glucose and lipid metabolism and insulin resistance in children.

Key words:

Obesity, Children, Serum complement C1q/tumor necrosis factor-related protein 3, Serum complement C1q/tumor necrosis factor-related protein 9, Glucose and lipid metabolism, Insulin resistance