Abstract:

Objective　To investigate whether circMBOAT2 affects the proliferation and apoptosis of lung cancer A549 cells by targeting miR-526b-3p. Methods　A total of 47 fresh lung cancer tissues and para-cancer tissues were collected from Shangluo Central Hospital from June 2021 to September 2023. Real-time quantitative PCR (qRT-PCR) was used to determine the expressions of circMBOAT2 and miR-526b-3p in lung cancer tissues. Lung cancer A549 cells were divided into a Con group ,a si-NC group (transfected with si-NC), a si-circMBOT2 group (transfected with si-circMBOT2), a pcDNA group (transfected with pcDNA), a pcDNA-circMBOAT2 group (transfected with pcDNA-circMBOAT2), a miR-NC group (transfected with miR-NC), a miR-526b-3p group (transfected with miR-526b-3p mimics), a si-circMBOAT2+anti-miR-NC group (co-transfected with si-circMBOAT2 and anti-miR-NC), a si-circMBOAT2+anti-miR-526b-3p group (co-transfected with si-circMBOAT2 and miR-526b-3p inhibitor). Cell proliferation, apoptosis, and protein expressions of Ki-67, Bcl-2, and Bax were measured by CCK-8, flow cytometry, and Western blotting. Dual luciferase reporter gene detection was used to analyze the targeting relationship between circMBOAT2 and miR-526b-3p. t test and SNK-q test were used. Results　The expression of circMBOAT2 in the lung cancer tissues was higher than that in the para-cancer tissues [(4.25±0.22) vs. (1.00±0.05)], and the expression of miR-526b-3p was lower than that in the para-cancer tissues [(0.33±0.04) vs. (1.00±0.07)], with statistically significant differences (t=98.76 and 56.97, both P<0.05). Interference with circMBOT2 or overexpression of miR-526b-3p inhibited the proliferation and protein expressions of Ki-67 and Bcl-2 of lung cancer A549 cells, and increased apoptosis rate and Bax protein expression (all P<0.05). circMBOAT2 targeted to regulate the expression of miR-526b-3p, and inhibition of miR-526b-3p expression reversed the effect of interfering circMBOAT2 expression on the proliferation and apoptosis of lung cancer A549 cells. Conclusion　circMBOAT2 is highly expressed in lung cancer tissues, and promotes the proliferation and inhibits apoptosis of lung cancer A549 cells by targeting the expression of miR-526b-3p.

Key words:

Lung cancer, circMBOAT2, miR-526b-3p, Proliferation, Apoptosis

摘要：

目的　探讨circMBOAT2是否通过靶向miR-526b-3p影响肺癌A549细胞的增殖和凋亡。方法　收集2021年6月至2023年9月在商洛市中心医院行手术治疗的47份新鲜肺癌组织和癌旁组织。实时定量PCR（qRT-PCR）测定肺癌组织中circMBOAT2和miR-526b-3p表达。肺癌A549细胞分为Con组、si-NC组、si-circMBOT2组、pcDNA组、pcDNA-circMBOAT2组、miR-NC组、miR-526b-3p组、si-circMBOAT2+anti-miR-NC组、si-circMBOAT2+anti-miR-526b-3p组。运用CCK-8、流式细胞术和免疫印迹实验分别测定细胞增殖、凋亡与Ki-67、Bcl-2、Bax蛋白表达。双荧光素酶报告基因检测分析circMBOAT2与miR-526b-3p靶向。统计学方法采用t检验、SNK-q检验。结果　肺癌组织中circMBOAT2表达高于癌旁组织［（4.25±0.22）比（1.00±0.05）］，miR-526b-3p表达低于癌旁组织［（0.33±0.04）比（1.00±0.07）］，差异均有统计学意义（t=98.76、56.97，均P<0.05）。干扰circMBOT2或过表达miR-526b-3p能抑制肺癌A549细胞增殖及Ki-67、Bcl-2蛋白表达，并增加凋亡率、Bax蛋白表达（均P<0.05）。circMBOAT2靶向调控miR-526b-3p的表达，抑制miR-526b-3p表达逆转了干扰circMBOAT2表达对肺癌A549细胞增殖和凋亡的作用。结论　circMBOAT2在肺癌组织中高表达，通过靶向调控miR-526b-3p表达，促进肺癌A549细胞的增殖和抑制凋亡。

关键词:

肺癌, circMBOAT2, miR-526b-3p, 增殖, 凋亡