关键词:

糖尿病性胃轻瘫, 平滑肌, 连接蛋白43, 变化, 动物实验

Abstract:

Objective　To study the dynamic changes of connexin 43 (Cx43) in gastric smooth muscle tissue of diabetic gastroparesis rats, to elucidate the pathogenesis of diabetic gastroparesis, and to provide experimental basis for drug development and prevention of diabetic gastroparesis and other diabetic smooth muscle lesions. Methods　A total of 40 adult male SD rats, weighing (200±20) g, were selected as the study subjects, in the School of Medicine of Yanbian University from March to July 2023. Ten rats of the normal control group were intraperitoneally injected the same amount of saline. The 0.5% streptozotocin solution was prepared with pH 4.0 citrate buffer (the concentration was 0.1 mol/L), and 65 mg/kg of streptozotocin solution was injected into the abdomen at one time. Seven days after intraperitoneal administration, caudal vein blood was removal, and blood glucose concentration was detected, blood glucose concentration 350 mg/dl as diabetic rats were successfully modeled. They were killed after feeding for 4 w (DM4w group), 6 w (DM6w group), and 8 w (DM8w group), with 10 rats in each group. The expressions of Cx43 protein in the total protein, plasma protein, and membrane protein of gastric smooth muscle tissue of rats in each group were detected by Western blot. t test was used for statistical analysis. Results　The expression of Cx43 in gastric smooth muscle tissue of rats in the normal control group was 1.14±0.15, 1.15±0.14 in the DM4w group, 1.33±0.15 in the DM6w group, and 1.70±0.22 in the DM8w group. The expression of CX43 in the DM8w group was higher than those in the normal control group and DM4w group (t=2.133 and 2.174, both P0.05). At the same time, the membrane expression and plasma expression of Cx43 were detected, and the membrane-to-plasma ratio was calculated. The membrane-to-plasma ratio of Cx43 in the normal control group was 0.50±0.07, 1.18±0.10 in the DM4w group, 3.13±0.40 in the DM6w group, and 2.39±0.21 in the DM8w group. The membrane-to-plasma ratio in the DM4w group was higher than that in the normal control group (t=5.745, P0.01), and those in the DM6w group and DM8w group were higher than that in the DM4w group (t=4.703 and 5.322, both P0.01). Conclusion　Dynamic changes in Cx43 expression and membrane repositioning in gastric smooth muscle tissue of diabetic rats serve as a cause of diabetic gastroparesis.

Key words:

Diabetic gastroparesis, Smooth muscle, Connexin 43, Change, Animal experiment