Abstract:

Objective　To investigate the significance of peripheral blood miR-126 in the progression of respiratory syncytial virus (RSV)-infected bronchiolitis to bronchial asthma. Methods　A total of 126 children with RSV-infected bronchiolitis admitted to Children's Hospital Affiliated to Zhengzhou University from June 2018 to June 2019 were collected, including 77 males and 49 females. The relative expression level of miR-126 was detected by RT-PCR. According to the results of 2-year follow-up, they were divided into a non-asthmatic group (94 cases) and an asthmatic group (32 cases). The receiver operating characteristic curve (ROC) was used to analyze the prognostic value of miR-126 in the progression of RSV-infected bronchiolitis to bronchial asthma, and logistic regression analysis was used to analyze whether miR-126 was an influencing factor in the progression of RSV-infected bronchiolitis to bronchial asthma. χ² test and independent sample t test were used. Results　The expression level of miR-126 in peripheral blood of the asthmatic group was significantly higher than that of the non-asthmatic group [(8.43±5.14) vs. (5.03±3.88)] (P<0.001). ROC analysis showed that the area under the curve of miR-126 was 0.691, the sensitivity was 88.30%, and the specificity was 59.38% in predicting the progression of RSV-infected bronchiolitis to bronchial asthma. miR-126 was an independent risk factor of the progression of RSV-infected bronchiolitis to bronchial asthma [OR=9.878 (3.752-26.007), P<0.001]. Conclusion　miR-126 in peripheral blood has a certain predictive value for the progression of children with RSV-infected bronchiolitis to bronchial asthma.

Key words:

miR-126, Respiratory syncytial virus, Bronchiolitis, Asthma

摘要：

目的　探讨外周血miR-126在呼吸道合胞病毒（RSV）感染毛细支气管炎患儿进展为支气管哮喘中的意义。方法　收集郑州大学附属儿童医院2018年6月至2019年6月收治的RSV毛细支气管炎患儿126例，其中男77例、女49例。采用RT-PCR法检测miR-126相对表达水平，根据2年随访结果将其分为非哮喘组（94例）与哮喘组（32例），受试者工作特征曲线（ROC）分析miR-126在RSV毛细支气管炎进展为哮喘中的预后价值，logistic回归分析miR-126是否是RSV毛细支气管炎进展为哮喘的影响因素。采用χ²检验、独立样本t检验。结果　哮喘组患儿外周血miR-126表达水平显著高于非哮喘组［（8.43±5.14）比（5.03±3.88）］（P<0.001）；ROC分析显示miR-126预测RSV毛细支气管炎进展为哮喘的曲线下面积为0.691，灵敏度为88.30%，特异度为59.38%；miR-126是RSV毛细支气管炎进展为支气管哮喘的独立危险因素［OR=9.878（3.752～26.007），P<0.001］。结论　外周血中miR-126对于RSV毛细支气管炎患儿进展为支气管哮喘具有一定预测价值。

关键词:

miR-126, 呼吸道合胞病毒, 毛细支气管炎, 哮喘