Effects of Acorus tatarinowii, Polygala tenuifolia, and their
compatibility on ox-LDL-induced injury of human umbilical vein endothelial
cells

doi:10.3760/cma.j.issn.1007-1245.2022.09.004

International Medicine and Health Guidance News ›› 2022, Vol. 28 ›› Issue (9): 1199-1203.DOI: 10.3760/cma.j.issn.1007-1245.2022.09.004

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Effects of Acorus tatarinowii, Polygala tenuifolia, and their compatibility on ox-LDL-induced injury of human umbilical vein endothelial cells

Chen Zhining, Liu Xiaojun, Tang Liang, Liang Weihai, Lin Guowei

Department of Traditional Chinese Medicine, Guangzhou First People's Hospital, Guangzhou 511458, China

Received:2022-02-17 Online:2022-05-01 Published:2022-05-11
Contact: Liu Xiaojun, Email: alxj@163.com
Supported by:
Project of Scientific and Technological Plan in Guangdong (2017A020213037);

Scientific Research Project of Guangdong Bureau of Traditional Chinese Medicine (20191266)

石菖蒲、远志及其配伍对ox-LDL诱导人脐静脉内皮细胞损伤的影响

陈志宁刘晓俊唐梁梁伟海林国伟

广州市第一人民医院中医科，广州　511458

通讯作者: 刘晓俊，Email：alxj@163.com
基金资助:
广东省科技计划项目（2017A020213037）；

广东省中医药局科研项目（20191266）

Abstract

Abstract: Objective　To investigate the effects of Acorus tatarinowii, Polygala tenuifolia, and their compatibility on ox-LDL-induced injury of human umbilical vein endothelial cells (HUVECs). Methods　From January to August 2021, HUVECs were cultured, and were randomly divided into a blank group, a model group, and sample groups. The different concentrations (5, 10, and 20 mg/ml) of Acorus tatarinowii, Polygala tenuifolia, and their compatibility were added into the sample groups. The HUVECs proliferation, relative viability test, apoptosis, cell cycle, and expressions of apoptotic proteins Bax and Bcl-2 were detected. One-way ANOVA was applied. Results　Acorus tatarinowii, Polygala tenuifolia, and their ompatibility could reduce the apoptosis of HUVECs [the blank group (0.338±0.001), the model group (4.319±0.124), the Acorus tatarinowii group (2.694±0.561), the Polygala tenuifolia group (2.278±0.914), and the Acorus tatarinowii + Polygala tenuifolia group (2.250±0.699)], and increase HUVECs cycle (S+G2)%. Both Acorus tatarinowii and Polygala tenuifolia could down-regulate the apoptotic protein Bax and inhibit the up-regulation of the apoptotic protein Bcl-2, and their compatibility was better than Acorus tatarinowii and Polygala tenuifolia. Conclusion　Acorus tatarinowii, Polygala tenuifolia, and their ompatibility can inhibit ox-LDL induced HUVECs injury, and their compatibility is stronger than single Chinese medicine.

Key words: Acorus tatarinowii, Polygala tenuifolia, Compatibility, HUVECs

摘要： 目的　观察石菖蒲、远志及其配伍对氧化型低密度脂蛋白（ox-LDL）诱导人脐静脉内皮细胞（HUVECs）损伤情况的影响。方法　2021年1月至8月，培养HUVECs，随机分为空白组、模型组和样品组，样品组中分别加入不同浓度（5、10、20 mg/ml）的石菖蒲、远志或石菖蒲+远志，对HUVECs增殖、相对活率试验、细胞凋亡、细胞周期、凋亡蛋白Bax和Bcl-2表达进行检测。采用单因素方差分析。结果　石菖蒲、远志及其配伍均能减少HUVECs的凋亡［空白组（0.338±0.001），模型组（4.319±0.124），石菖蒲组（2.694±0.561），远志组（2.278±0.914），石菖蒲+远志组（2.250±0.699）］；石菖蒲、远志及其配伍均能增加HUVECs周期（S+G2）%；远志、石菖蒲均能使凋亡相关蛋白Bax下调，同时抑制凋亡相关蛋白Bcl-2上调，远志+石菖蒲配伍下对Bax及Bcl-2蛋白的影响优于单味中药石菖蒲、远志。结论　石菖蒲、远志及其配伍均能抑制ox-LDL诱导HUVECs损伤，其配伍作用强于单味中药。

关键词: 石菖蒲, 远志, 配伍, 人脐静脉内皮细胞

Chen Zhining, Liu Xiaojun, Tang Liang, Liang Weihai, Lin Guowei. Effects of Acorus tatarinowii, Polygala tenuifolia, and their compatibility on ox-LDL-induced injury of human umbilical vein endothelial cells[J]. International Medicine and Health Guidance News, 2022, 28(9): 1199-1203.

陈志宁, 刘晓俊, 唐梁, 梁伟海, 林国伟. 石菖蒲、远志及其配伍对ox-LDL诱导人脐静脉内皮细胞损伤的影响[J]. 国际医药卫生导报, 2022, 28(9): 1199-1203.

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Effects of Acorus tatarinowii, Polygala tenuifolia, and their compatibility on ox-LDL-induced injury of human umbilical vein endothelial cells

石菖蒲、远志及其配伍对ox-LDL诱导人脐静脉内皮细胞损伤的影响

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