Abstract:

Objective　To determine the content difference between the main components of Phellodendrine and Berberine in different processing methods, and to discuss the effect of processing on the quality of Cortex Phellodendri. Methods　The contents of Phellodendrine and Berberine in different processing methods were determined by high performance liquid chromatography (HPLC). Using Diamonsil C₁₈ (250.0 mm×4.6 mm, 5 µm) as column, acetonitrile-0.1% formic acid as gradient elution, the flow rate was 1.0 ml·min^-1, the column temperature was 25 ℃, and the detection wavelength was 284 nm. Results　Phellodendrine and Berberine were well related linearly within the mass concentration of 0.354-0.760, 3.865-7.530 g·L^-1 (R²>0.999 0). The contents of Phellodendrine and Berberine in different processed products of Cortex Phellodendri were different. The contents of Phellodendrine and Berberine in wine Phellodendri were the highest, which were 1.360 mg·g^-1 and 10.200 mg·g^-1. The order from low to high of Phellodendrine content was as follows: Phellodendri charcoal < raw Phellodendri < salt Phellodendri < wine Phellodendri; the order from low to high of Berberine content was as follows: Phellodendri charcoal < salt Phellodendri < raw Phellodendri < wine Phellodendri. Conclusions　Different processing methods affect the main active components of Phellodendrine and Berberine, and then its pharmacodynamics. The method is simple, scientific, and reasonable, which could be used for further study of Cortex Phellodendri.

Key words:

Cortex Phellodendri, Processing, Phellodendrine, Berberine, HPLC, Content determination

摘要：

目的　测定不同黄柏炮制品中主要成分黄柏碱和小檗碱之间的含量差异，以探讨不同炮制方法对该药材质量的影响。方法　采用高效液相色谱法（HPLC）对不同黄柏炮制品中的黄柏碱和小檗碱进行含量测定，以Diamonsil C₁₈（250 mm×4.6 mm，5 µm）为色谱柱，乙腈-0.1%甲酸为流动相，梯度洗脱，流速为1.0 ml·min^-1，柱温25 ℃，检测波长为284 nm。结果　黄柏碱和小檗碱线性范围分别为0.354～0.760、3.865～7.530 g·L^-1，线性关系良好，均R²>0.999 0。黄柏不同炮制品中黄柏碱和小檗碱含量差异较大，酒黄柏的黄柏碱与小檗碱含量最高，分别为1.360 mg·g^-1、10.200 mg·g^-1，黄柏碱含量由低到高顺序为：黄柏炭<黄柏生品<盐黄柏<酒黄柏，小檗碱含量由低到高顺序为黄柏炭<盐黄柏<黄柏生品<酒黄柏。结论　不同炮制方法会对黄柏的主要有效成分黄柏碱和小檗碱产生影响，且可能影响药效。本研究方法简便、科学合理，可为深入研究黄柏提供参考。

关键词:

黄柏, 炮制, 黄柏碱, 小檗碱, 高效液相色谱法, 含量测定