Application of network pharmacology to elucidate the potential mechanism of Astragalus - Scutellaria barbata D.Don (SBD) against colorectal cancer

doi:10.3760/cma.j.issn.1007-1245.2023.19.017

Abstract

Abstract:

Objective　To explore the effective components and molecular mechanism of Astragalus - Scutellaria barbata D.Don (SBD) in the treatment of colorectal cancer based on network pharmacology analysis methods. Methods　The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) was used to search for the effective components and targets of action of Astragalus and SBD. Colorectal cancer related targets were screened through databases such as GeneCards. A drug component potential target network relationship diagram was constructed using Cytoscape (Version 3.9.1) software. A protein-protein interaction (PPI) network diagram was built through the String database platform, and the functional and signal pathway enrichment analysis on potential targets were performed by the Metascape database. Results　Through database screening, 17 active components of Astragalus and 28 active components of SBD were identified. A total of 24 potential protein targets were intersected between Astragalus-SBD and colorectal cancer targets. The gene ontology (GO) enrichment analysis showed that Astragalus-SBD was mainly related to biological functions such as DNA damage, cell apoptosis, and enzyme activity regulation. The Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis showed that the main pathways included PI3K/AKT signaling pathway, mitogen activated protein kinase (MAPK) signaling pathway, tumor necrosis factor (TNF) signaling pathway, etc. Conclusions　The effective ingredients and potential mechanism of Astragalus-SBD in the treatment of colorectal cancer are reflected through network pharmacology analysis methods. It may intervene in colorectal cancer through multiple components, targets, and pathways, providing a theoretical basis for the treatment of colorectal cancer with Astragalus-SBD.

Key words:

Astragalus, Scutellaria barbata D.Don, Colorectal cancer, Signal pathway, Network pharmacology

摘要：

目的　基于网络药理学分析方法探讨黄芪-半枝莲抗结直肠癌的有效成分及分子机制。方法　运用中药药理学分析平台数据库（TCMSP）检索黄芪与半枝莲的有效成分及作用靶点，通过GeneCards数据库等筛选结直肠癌相关靶点，运用Cytoscape（Version 3.9.1）软件构建药物-成分-潜在靶点网络关系图。通过String数据库平台构建蛋白相互作用（protein-protein interaction，PPI）网络图，并用Metascape数据库对潜在靶点进行功能和信号通路富集分析。结果　通过数据库筛选出黄芪有效成分17种，半枝莲有效成分28种，黄芪-半枝莲与结直肠癌靶点交集共24个潜在蛋白靶点。基因本体论（gene ontology，GO）富集分析显示黄芪-半枝莲主要与DNA损伤、细胞凋亡、调节酶活性等生物学功能相关，京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes，KEGG）富集分析显示主要通路包括磷脂酰肌醇3-激酶/蛋白激酶B（PI3K/AKT）信号通路、丝裂原活化蛋白激酶（MAPK）信号通路、肿瘤坏死因子（TNF）信号通路等。结论　通过网络药理学分析方法体现了黄芪-半枝莲抗结直肠癌的有效成分及潜在作用机制，黄芪-半枝莲可能通过多种成分、多个靶点、多种途径干预结直肠癌，为黄芪-半枝莲治疗结直肠癌提供理论依据。

关键词:

黄芪, 半枝莲, 结直肠癌, 信号通路, 网络药理学

Li Jingchun, Long Qixiong, Feng Feng, Sun Wei.

Application of network pharmacology to elucidate the potential mechanism of Astragalus - Scutellaria barbata D.Don (SBD) against colorectal cancer [J]. International Medicine and Health Guidance News, 2023, 29(19): 2737-2745..

李静纯龙其雄冯峰孙伟.

基于网络药理学方法探讨黄芪-半枝莲抗结直肠癌的作用机制 [J]. 国际医药卫生导报, 2023, 29(19): 2737-2745..

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