老年脑卒中血浆同型半胱氨酸与叶酸代谢基因表达及相关性分析

doi:10.3760/cma.j.cn441417-20250509-22004

摘要/Abstract

摘要： 目的　探讨老年脑卒中血浆同型半胱氨酸（homocysteine，Hcy）与叶酸代谢基因表达及相关性。方法　选择2021年1月至2023年12月徐州市第一人民医院收治的老年脑卒中患者75例为研究组，选取同期健康体检者60例为对照组。研究组男47例，女28例，年龄（71.85±5.06）岁。对照组男39例，女21例，年龄（72.09±5.24）岁。比较两组亚甲基四氢叶酸还原酶（methylenetetrahydrofolate reductase，MTHFR）的C677T、MTRR的A66G位点基因型及等位基因型。比较两组血浆Hcy水平。比较MTHFR的C677T、MTRR的A66G位点不同基因型血浆Hcy水平。logistic回归分析MTHFR、MTRR基因型、血浆Hcy水平与老年脑卒中发生的关系。采用t检验和χ²检验进行统计分析。结果　研究组、对照组MTHFR的C677T、MTRR的A66G基因多态性差异有统计学意义（均P<0.05）。研究组T及G等位基因型频率均高于对照组（均P<0.05）。研究组Hcy水平高于对照组［（34.10±4.83）μmol/L比（15.87±3.86）μmol/L，P<0.05］。MTHFR的C677T位点TT基因型患者血浆Hcy水平高于CC、CT基因型患者，MTRR的A66G位点GG基因型患者血浆Hcy水平高于AA、AG基因型患者（均P<0.05）。多因素分析显示，MTHFR的C677T、MTRR的A66G基因多态性分布及血浆Hcy水平是老年脑卒中的独立影响因素（均P<0.05）。结论　MTHFR的C677T、MTRR的A66G基因多态性分布及血浆Hcy水平是老年脑卒中的独立影响因素。

关键词: 脑卒中, 同型半胱氨酸, 叶酸代谢基因, 基因多态性

Abstract: Objective　To investigate the expression and correlation of plasma homocysteine (Hcy) and folic acid metabolism genes in elderly stroke patients. Methods　Seventy-five elderly stroke patients treated in Xuzhou First People's Hospital from January 2021 to December 2023 were selected as a study group, and 60 healthy individuals undergoing physical examinations during the same period were selected as a control group. There were 47 males and 28 females in the study group; they were (71.85±5.06) years old. There were 39 males and 21 females in the control group; they were (72.09±5.24) years old. The genotypes and allelic genotypes of C677T of MTHFR and A66G of MTRR were compared between the two groups. The plasma Hcy levels were compared between the two groups. The plasma Hcy levels of different genotypes of C677T of MTHFR and A66G of MTRR were compared. The logistic regression analysis was used to analyze the relationship of MTHFR, MTRR genotypes, and plasma Hcy level with the occurrence of stroke in the elderly. t and χ² tests were used for the statistical analysis. Results　There were statistical differences in the polymorphisms of the C677T gene of MTHFR and the A66G gene of MTRR between the study group and the control group (both P<0.05). The frequencies of T and G allele genotypes in the study group were higher than those in the control group (both P<0.05). The Hcy level in the study group was higher than that in the control group [(34.10±4.83) μmol/L vs. (15.87±3.86) μmol/L; P<0.05]. The plasma Hcy level of the patients with TT genotype at the C677T locus of MTHFR was higher than those of the patients with CC and CT genotypes, and the plasma Hcy level of the patients with GG genotype at the A66G locus of MTRR was higher than those of the patients with AA and AG genotypes (both P<0.05). The multivariate analysis showed that the distribution of C677T gene polymorphisms of MTHFR and A66G gene polymorphisms of MTRR, as well as the plasma Hcy level, were independent influencing factors for elderly stroke (all P<0.05). Conclusion　The distribution of C677T gene polymorphisms of MTHFR and A66G gene polymorphisms of MTRR, as well as the plasma Hcy level, are independent influencing factors for elderly stroke.

Key words: Stroke, Homocysteine, Olic acid metabolism gene, Gene polymorphism

钮威杨芳. 老年脑卒中血浆同型半胱氨酸与叶酸代谢基因表达及相关性分析[J]. 国际医药卫生导报, 2025, 31(22): 3714-3717.

Niu Wei, Yang Fang. Expression and correlation analysis of plasma homocysteine and folic acid metabolism genes in elderly stroke patients[J]. International Medicine and Health Guidance News, 2025, 31(22): 3714-3717.

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