关键词:

细针抽吸活检, 超声, 基因突变检测, 细胞学检查, 甲状腺结节

Abstract:

Objective　To explore the diagnostic value of serine/threonine specific kinase gene V600E (BRAF V600E) detection combined with ultrasound-guided fine needle aspiration biopsy (US-FNAB) in thyroid nodules, so as to provide reference for early clinical qualitative diagnosis. Methods　A total of 90 patients with suspected malignant thyroid nodules in Chengwu County People's Hospital from August 2019 to August 2022 were selected as the study subjects, including 51 males and 39 females, aged (56.27±2.84) years. After admission, the BRAF V600E gene mutation detection and US-FNAB examination were performed. With the surgical or pathological biopsy results as the gold standard, the diagnostic efficacies of BRAF V600E gene mutation detection and US-FNAB alone and combined were compared. The BRAF V600E gene mutation types and US-FNAB classification in patients with different thyroid nodule diameters were compared. χ² test was used. Results　The results of operation or pathological biopsy showed that among the 90 patients with suspected malignant thyroid nodules, 62 cases were malignant and 28 cases were benign. The results of BRAF V600E gene mutation detection showed that 51 cases were malignant and 39 cases were benign. The results of US-FNAB showed that 50 cases were malignant and 40 cases were benign. The combined diagnosis showed that 61 cases were malignant and 29 cases were benign. Compared with BRAF V600E gene mutation detection and US-FNAB diagnosis alone, the sensitivity and accuracy of combined diagnosis were higher at 95.08% (59/62) and 94.38% (85/90), while the missed diagnosis rate was lower at 4.92% (3/62) (all P<0.05). There were no statistically significant differences in the BRAF V600E gene mutation types among patients with different thyroid nodule diameters (all P>0.05). There were statistically significant differences in the class Ⅲ and class Ⅵ by US-FNAB examination among patients with different thyroid nodule diameters, among which class Ⅵ was more significantly different (all P<0.05). Conclusion　BRAF V600E gene mutation detection combined with US-FNAB examination can effectively improve the diagnostic accuracy of thyroid nodules, which is conducive to early qualitative diagnosis and can provide a basis for early clinical intervention.

Key words:

Fine needle aspiration biopsy, Ultrasound, Gene mutation detection, Cytological examination, Thyroid nodules