关键词:

鼻咽癌, miR-29c-3p, miR-143-5p, miR-205-5p, 临床预后

Abstract:

Objective　To explore the value of the levels of miR-29c-3p, miR-143-5p, and miR-205-5p in the prediction of the clinical prognosis of patients with nasopharyngeal carcinoma. Methods　This was a prospective study. One hundred and twenty-six patients with nasopharyngeal carcinoma diagnosised and treated at Second Hospital, Air Force Medical University from January 2019 to April 2022 were selected as an observation group, including 90 males and 36 females who were (47.65 7.97) years old. Fifty healthy examinees during the same period were selected as a control group, including 31 males and 19 females who were (45.38±8.53) years old. The expression levels of miR-29c-3p, miR-143-5p, and miR-205-5p in the biopsy tissues from both groups were measured by reverse transcription polymerase chain reaction (RT-PCR), and their correlation with the patients' clinical prognosis was explored. The survival curve was drawn by the Kaplan-Meier method, the Log-Rank test was used. The measurement data of normal distribution were compared between the groups by t test, and the count data by χ² test. Results　The levels of miR-29c-3p and miR-143-5p in the observation group were lower than those in the control group (2.68±0.64 vs. 5.86±1.95 and 1.83±0.49 vs. 5.04±1.67); the level of miR-205-5p in the observation group was higher than that in the control group (4.81±1.68 vs. 3.89±1.08); there were statistical differences (t=16.284, 19.623, and 4.302; all P<0.05). The levels of miR-29c-3p, miR-143-5p, and miR-205-5p were not correlated with gender, age, T stage, lymph node metastasis, distant metastasis, and clinical stage (all P>0.05). The Kaplan-Meier survival curves showed that the 2-year overall survival rate in the high-expression group of miR-205-5p was lower than that in the low-expression group of miR-205-5p [76.19% (48/63) vs. 90.48% (57/63)], with a statistical difference (χ²=4.629; P<0.05). The 2-year overall survival rates in the high-expression groups of miR-29c-3p and miR-143-5p were higher than those in the low-expression groups of miR-29c-3p and miR-143-5p [93.65% (59/63) vs. 73.02% (46/63) and 95.24% (60/63) vs. 71.43% (45/63)], with statistical differences (χ²=9.657 and 12.857; both P<0.05). Conclusions　The expressions of miR-29c-3p and miR-143-5p are reduced, while the expression of miR-205-5p is increased in patients with nasopharyngeal carcinoma. These expression patterns are significantly correlated with patient survival rates and can serve as prognostic biomarkers.

Key words:

Nasopharyngeal carcinoma, Mir-29C-3P, Mir-143-5P, Mir-205-5p, Clinical prognosis