PPP1R1A对大鼠胰腺INS-1细胞作用的分子机制

doi:10.3760/cma.j.cn441417-20240318-10015

国际医药卫生导报 ›› 2025, Vol. 31 ›› Issue (10): 1656-1659.DOI: 10.3760/cma.j.cn441417-20240318-10015

PPP1R1A对大鼠胰腺INS-1细胞作用的分子机制

冼恒标¹ 林德健¹ 许名颖² 陈培海² 蒋红群² 陈立强²

¹肇庆医学院附属口腔医院，肇庆 526020；²肇庆医学院，肇庆 526020

收稿日期:2024-03-18 出版日期:2025-05-15 发布日期:2025-05-21
通讯作者: 陈立强，Email：LQchen81@163.com
基金资助:
广东省大学生科技创新培育专项资金面上项目（pdjh2021b0991）；广东省教育厅项目（2020KTSCX359）；肇庆市科技创新计划（2021040314026）

Molecular mechanism of PPP1R1A in rat pancreatic INS-1 cells

Xian Hengbiao¹, Lin Dejian¹, Xu Mingying², Chen Peihai², Jiang Hongqun², Chen Liqiang²

¹Affiliated Stomatological Hospital of Zhaoqing Medical College, Zhaoqing 526020, China; ²Zhaoqing Medical College, Zhaoqing 526020, China

Received:2024-03-18 Online:2025-05-15 Published:2025-05-21
Contact: Chen Liqiang, Email: LQchen81@163.com
Supported by:
Project Funded by Special Program for Training and Culturing College Students at Scientific and Technological Innovation in Guangdong (pdjh2021b0991); Project of Guangdong Education Department (2020KTSCX359); Innovative Plan of Science and Technology in Zhaoqing (2021040314026)

摘要/Abstract

摘要：

目的　探讨蛋白质磷酸酶1调节亚基1A（protein phosphatase 1 regulatory subunit 1A，PPP1R1A）对大鼠胰腺瘤细胞株（INS-1）的细胞作用机制及影响机制。方法　转染pLenti-PPP1R1A-sgRNA质粒于大鼠胰腺瘤INS-1细胞，Western blot检测PPP1R1A、Fox01、NKX6.1、MAFA的表达水平，CCK-8法检测各组细胞活性，酶联免疫吸附试验检测细胞上清液中培养细胞分泌的胰岛素的浓度。采用方差分析和t检验进行统计分析。结果　pLenti-PPP1R1A-sgRNA质粒转染瘤细胞后，对照组PPP1R1A、Fox01、NKX6.1、MAFA表达水平分别为0.382±0.187、0.311±0.151、0.224±0.053和0.317±0.184，而实验组分别为0.156±0.032、0.203±0.099、0.104±0.022和0.193±0.081（均P<0.05）。CCK-8法结果显示，pLenti-PPP1R1A-sgRNA质粒转染细胞后INS-1细胞增殖抑制（P<0.05），而且INS-1细胞分泌胰岛素的功能下降（P<0.05）。结论　抑制PPP1R1A基因的活性可导致INS-1细胞合成和分泌胰岛素的功能下降。该作用机制可能是2型糖尿病患者的患病过程中胰腺β细胞功能异常以及细胞去极化形成的机制之一。

关键词: 2型糖尿病, , , 蛋白质磷酸酶1调节亚基1A, , , 胰岛素分泌

Abstract:

Objective　To investigate the molecular mechanism of protein phosphatase 1 regulatory subunit 1A (PPP1R1A) of rat pancreatic INS-1 cells. Methods　The pLenti-PPP1R1A-sgRNA plasmid was transfected in the rat pancreatic INS-1 cells. The expression levels of PPP1R1A, MafA, Pdx1, and Pax6PPP1R1A, Fox01, NKX6.1, and MAFA were detected by Western blotting. The cell activity was detected by the CCK-8 assay. The concentration of insulin in the cell culture fluid was detected by the enzyme linked immunosorbent assay. Analysis of variance and t test were used for the statistical analysis. Results　After transfecting with pLenti-PPP1R1A-sgRNA plasmid, the expression levels of PPP1R1A, Fox01, NKX6.1, and MAFA in the control group were 0.382±0.187, 0.311±0.151, 0.224±0.053, and 0.317±0.184, and those in the experimental group were 0.156±0.032, 0.203±0.099, 0.104±0.022, and 0.193±0.081 (all P<0.05). The results of the CCK-8 assay showed that after transfecting with pLenti-PPP1R1A-sgRNA plasmid, the proliferation of the INS-1 cells was inhibited (P<0.05), and the insulin secretion function was decreased in the INS-1 cells (P<0.05). Conclusion　Inhibition of the PPP1R1A gene may affect the insulin synthesis and secretion function in INS-1 cells, which may be one of the abnormal and depolarization mechanisms of pancreatic β cells in patients with type 2 diabetes.

Key words: Type 2 diabetes, , Protein phosphatase 1 regulatory subunit 1A, , Insulin secretion

冼恒标林德健许名颖陈培海蒋红群陈立强. PPP1R1A对大鼠胰腺INS-1细胞作用的分子机制 [J]. 国际医药卫生导报, 2025, 31(10): 1656-1659.

Xian Hengbiao, Lin Dejian, Xu Mingying, Chen Peihai, Jiang Hongqun, Chen Liqiang.

Molecular mechanism of PPP1R1A in rat pancreatic INS-1 cells [J]. International Medicine and Health Guidance News, 2025, 31(10): 1656-1659.

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PPP1R1A对大鼠胰腺INS-1细胞作用的分子机制

Molecular mechanism of PPP1R1A in rat pancreatic INS-1 cells

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