Abstract: Objective　To explore the antibacterial effect of a traditional Chinese medicine compound preparation, Ze'er Fu Skin Bacteriostasis Lotion, and provide experimental basis for the clinical application of the lotion. Methods　The antibacterial effects of 100.0%, 50.0%, 25.0%, and 12.5% Ze'er Fu Skin Bacteriostasis Lotion for 2, 5, 10, and 20 min on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans were experimentally observed by the plate dilution method form september to November 2021 at Dermatology Hospital, Southern Medical University, with sterile saline as the blank control group. The data were expressed as (x±s). The experimental data were analyzed by variance analysis and correlation analysis. Results　The antibacterial rates of 100.0%, 50.0%, 25.0%, and 12.5% Ze'er Fu Skin Bacteriostasis Lotion for 2, 5, 10 and 20 minutes on Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus,and Candida albicans were all over 90.0%. When 100.0% Ze'er Fu Bacteriostasis Lotion was used for 2, 5, 10, and 20 minutes, the antibacterial rates were (0.97±0.02), (0.99±0.03), (1.00±0.00), (1.00±0.00), respectively; when 50.0% Ze'er Fu Antibacterial Lotion was used for 2, 5, 10, and 20 minutes, the antibacterial rates were (0.95±0.02), (0.96±0.02), (0.97±0.01), and (0.98±0.01), respectively; when 25.0% Ze'er Fu Bacteriostasis Lotion was used for 2, 5, 10, and 20 minutes, the antibacterial rates were (0.93±0.01), (0.94±0.01), (0.95±0.01), and (0.97±0.01), respectively; when 12.5% Ze'er Fu Bacteriostasis Lotion was used for 2, 5, 10, and 20 minutes, the antibacterial rates were (0.92±0.01), (0.93±0.01), (0.93±0.01), and (0.95±0.02), respectively, there were statistical differences in the antibacterial rates between Ze'er Fu Skin Bacteriostasis Lotion with different concentrations for 2, 5, 10 and 20 minutes (F=5.947, 7.030, 52.993, and 8.912; all P<0.05). The correlation analysis showed that the antibacterial rate was positively correlated with the concentration of antibacterial lotion, and the Pearson correlation coefficients of action for 2, 5, 10, and 20 min were 0.769, 0.789, 0.950, and 0.806, respectively (all P<0.01). At the same concentration, there were no statistical differences between the antibacterial rate and strain species and durations of action (all P>0.05). Conclusion　Ze'er Fu Skin Bacteriostasis Lotion has strong antibacterial effect on Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans, and the antibacterial effect increases with the concentration.

Key words: Ze'er Fu Skin Bacteriostasis Lotion, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Traditional Chinese medicine

摘要： 目的　探讨一种中药复合制剂-泽尔复皮肤抑菌洗剂的抑菌效果，为该洗剂的临床运用提供实验依据。方法　2021年9月至11月于南方医科大学皮肤病医院采用平皿稀释法实验性观察泽尔复皮肤抑菌洗剂原液（100.0%）、50.0%、25.0%和12.5%浓度，于作用时间：2 min、5 min、10 min、20 min，对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌和白色念珠菌的抑菌效果，以灭菌生理盐水为空白对照组。组内结果以均数±标准差（x±s)表述，多组间实验数据采用方差分析与相关分析。结果　泽尔复皮肤抑菌洗剂原液（100.0%）、50.0%、25.0%和12.5%稀释液作用2 min、5 min、10 min、20 min对大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和白色念珠菌的抑菌率均超90%以上。泽尔复皮肤抑菌洗剂原液作用2 min、5 min、10 min、20 min，抑菌率分别为（0.97±0.02）、（0.99±0.03）、（1.00±0.00）、（1.00±0.00）；50.0%稀释液作用2 min、5 min、10 min、20 min，抑菌率分别为（0.95±0.02）、（0.96±0.02）、（0.97±0.01）、（0.98±0.01）；25.0%稀释液作用2 min、5 min、10 min、20 min，抑菌率分别为（0.93±0.01）、（0.94±0.01）、（0.95±0.01）、（0.97±0.01）；12.5%稀释液作用2 min、5 min、10 min、20 min，抑菌率分别为（0.92±0.01）、（0.93±0.01）、（0.93±0.01）、（0.95±0.02），不同浓度泽尔复皮肤抑菌洗剂作用2 min、5 min、10 min、20 min抑菌率差异均有统计学意义（F=5.947、7.030、52.993、8.912，均P<0.05），相关分析显示抑菌率与抑菌洗剂浓度呈正相关，作用2 min、5 min、10 min、20 min Pearson相关系数分别为0.769、0.789、0.950、0.806（均P<0.05）。在相同浓度下，抑菌率与菌株种类、作用时间差异无统计学意义（均P>0.05）。结论　泽尔复皮肤抑菌洗剂对大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌和白色念珠菌均有较强的抑菌作用，且随着浓度增加抑菌作用增强。

关键词: 泽尔复皮肤抑菌洗剂, 大肠埃希氏菌, 金黄色葡萄球菌, 铜绿假单胞菌, 白色假丝酵母菌, 中药