Abstract: Objectives　To explore the protection of Astragaloside Ⅳ from intestinal ischemia reperfusion injury in rats and to observe its effect on the expression of vascular endothelial cell growth factor. Methods　From January to June 2017, fourty-five male Wistar rats were divided into a surgery group (group A), an ischemia-reperfusion group (group B), and an Astragaloside Ⅳ group (group C), with 15 ones in each group. Laparotomy was operated in group A; the mesenteric arteries in group B and group C were clipped for 45 min; 10 min before the arteries were clipped, group C were intraperitoneally injected Astragaloside Ⅳ 6.0 ml·kg^-1; 24 h later the rats were killed, and their intestine and serum tissues were detected; the intestine tissue was observed under light microscope after HE staining; serum diamine oxidase (DAO) and D-lactate were measured by enzyme-linked immunosorbent assay (ELISA); the expression of VEGF protein was detected by Western blot. One-way ANOVA was used for the comparison between the 3 groups. If P<0.05, there is a statistical difference. Results　Compared to group B, group A and group C had milder intestinal villus damage and better intestinal villus arrangement. The serum levels of D-lactate in group A, group B, and group C were (1.24±0.08) μg/L, (7.41±1.07) μg/L, and (5.78±0.48) μg/L, and the DAO levels were (2.27±0.19) IU/ml, (9.36±1.03) IU/ml, and (6.82±0.52) IU/ml, respectively, with statistical differences between group B on one hand and group A and group C on the other hand and between group A and group C (all P<0.05). The expressions of VEGF in group B and group C were higher than that in group A, and that in group C was the highest. Conclusion　Astragaloside Ⅳ protects intestine from ischemia reperfusion injury upregulating the expression of vascular endothelial cell growth factor.

Key words: Astragaloside Ⅳ, Intestine, Reperfusion injury, Vascular endothelial cell growth factor

摘要： 目的　探讨黄芪甲苷对大鼠肠道缺血再灌注损伤的保护作用，观察其对血管内皮细胞生长因子（VEGF）表达的影响。方法　2017年1月至6月选取健康雄性Wistar大鼠45只，分为手术组（A组）、缺血再灌注组（B组）及黄芪甲苷组（C组），每组15只。A组给予剖腹手术，B、C组夹闭肠系膜前动脉45 min，C组夹闭动脉前10 min预先腹腔注射黄芪甲苷6.0 ml·kg^-1，24 h后处死实验动物，取大鼠的小肠及血清组织进行检测。HE染色光镜下观察肠道组织病理变化；酶联免疫吸附测定（ELISA）检测血清D-乳酸、二胺氧化酶（DAO）水平；蛋白质印迹法（Western blot）检测VEGF蛋白的表达水平。多组间比较采用单因素方差分析，P<0.05为差异有统计学意义。结果　HE染色示：与B组比较，A、C两组大鼠的肠绒毛破坏较轻，绒毛排列整齐；ELISA结果示：A、B、C组的血浆D-乳酸分别为（1.24±0.08）μg/L、（7.41±1.07）μg/L、（5.78±0.48）μg/L，DAO水平分别为（2.27±0.19）IU/ml、（9.36±1.03）IU/ml、（6.82±0.52）IU/ml，B组血浆D-乳酸、DAO水平与A、C组比较，差异均有统计学意义（均P<0.05），A、C两组间比较差异有统计学意义（P<0.05）；Western blot示：VEGF蛋白在B、C两组表达明显上调，C组表达水平更高。结论　黄芪甲苷对肠道缺血再灌注损伤有保护作用，其机制可能通过上调VEGF蛋白的表达发挥作用。

关键词: 黄芪甲苷, 小肠, 再灌注损伤, 血管内皮生长因子