Abstract:

Objective　To analyze the differences in the structure of oral microbiota between patients with erectile dysfunction (ED) and healthy controls by 16S rRNA high-throughput sequencing, and to explore the relationship between ED and oral microbiota. Methods　Fifteen ED patients who were 20-65 years old and treated at Department of Urological Surgery, First Hospital, Guizhou University of Traditional Chinese Medicine (ED group) and 15 healthy controls (normal control group) were selected. Their saliva samples were collected for the 16S rRNA high-throughput sequencing. The structures of the bacterial flora in the saliva samples of both groups were compared and analyzed. The independent-sample t test, non-parameter test, and χ² test were applied. Results　The compositions of core dominant bacteria in the salivary microbiota of the ED group and the normal control group were similar; the top ten dominant bacteria at the genus level were Fusobacterium, Reil, Neisseria, Haemophilus, Prevotella, Veillonella, Isovalinus, Streptococcus, Porphyromonas, and Actinobacteria. There were statistical differences in the abundances of different flora in the saliva samples between the two groups (all P<0.05). The abundance of Streptococcus in the ED group was significantly higher than that in the normal control group [8.59 (4.13, 11.75)% vs. 4.23 (3.25, 7.48)%], while the abundance of Fusobacterium in the ED group was significantly lower than that in the normal control group [9.38 (5.61, 14.91)% vs. 14.13 (7.92, 19.03)%], with statistical differences between the two groups (both P<0.05). Conclusions　The oral microbiota of ED patients and healthy people have the same core composition skeleton, but there are some differences in the microbiota structure, which may be closely related to the occurrence and development of ED. However, the causal relationship and its related mechanisms should be further explored.

Key words:

Erectile dysfunction, 16S rRNA, High-throughput sequencing, Oral flora

摘要：

目的　通过16S rRNA高通量测序分析勃起功能障碍（erectile dysfunction，ED）患者和正常健康人的口腔菌群结构差异，探讨ED与口腔菌群的关系。方法　选择贵州中医药大学第一附属医院泌尿外科门诊就诊的年龄段在20～65岁的ED患者（ED组）与健康者（正常对照组），各15例，收集唾液样本分别进行16S rRNA高通量测序，对各组唾液样本菌群的结构进行比较和分析。采用独立样本t检验、非参数检验、χ²检验进行统计分析。结果　ED组和正常对照组唾液中菌群的核心优势菌种组成相似，在菌属水平上优势菌属前10位分别为梭杆菌属、雷尔氏菌属、奈瑟氏菌属、嗜血杆菌属、普雷沃-7菌属、韦荣氏球菌属、异戊酸杆菌、链球菌属、卟啉菌属、放线菌属。两组唾液样本中不同菌群的丰度差异均有统计学意义（均P<0.05）。ED组中的链球菌属的丰度高于正常对照组［8.59（4.13，11.75）%比4.23（3.25，7.48）%］，梭杆菌属的丰度低于正常对照组［9.38（5.61，14.91）%比14.13（7.92，19.03）%］，两组比较差异均有统计学意义（均P<0.05）。结论　ED患者与健康人的口腔菌群存在着相同的核心组成骨架，而菌群结构却存在一定的差异，这或许和ED的发生发展有着密切联系，但其中的因果关系及其相关机制还有待进一步深入探讨。

关键词:

勃起功能障碍, 16S rRNA, 高通量测序, 口腔菌群