Abstract:

Objective　To explore the expression patterns and clinical values of Wnt10a, Wnt10b, Wnt5a, and β-catenin in cutaneous squamous cell carcinoma (SCC) and basal cell carcinoma (BCC). Methods　Thirty-three specimens from the patients with SCC and 33 specimens from the patients with BCC pathologically confirmed at Hospital Affiliated to Jiujiang University from January to December 2021 were selected. There were 28 males and 38 females; they were 45-88 years old. The expressions of Wnt10a, Wnt10b, Wnt5a, and β-catenin in the specimens were detected by the immunohistochemical staining. The χ² test was used. Results　Wnt10b, Wnt5a, and β-catenin expressed mainly in the cytoplasm and nucleus in both BCC and SCC; the positive expression rates in both tumors were 100.0%. Wnt10a did not expressed in BCC, while the positive rate in SCC was 30.3% (10/33), with a statistical difference (P<0.05). Conclusions　Wnt signaling pathway proteins obviously express in BCC and SCC, indicating that the Wnt signaling pathway plays a significant role in the tumorigenesis and development of BCC. Wnt10a can be used as a molecular marker identifying BCC and SCC.

Key words:

Basal cell carcinoma, Squamous cell carcinoma, Wnt, Immunohistochemistry

摘要：

目的　探究Wnt10a、Wnt10b、Wnt5a和β-连环蛋白（β-catenin）在皮肤鳞状细胞癌（SCC）和基底细胞癌（BCC）中的表达模式及临床意义。方法　选取2021年1月至12月于九江学院附属医院确诊为SCC和BCC的患者标本各33例，其中男28例，女38例，年龄45～88岁。使用免疫组织化学染色方法检测样本中Wnt10a、Wnt10b、Wnt5a、β-catenin表达情况。采用χ²检验。结果　Wnt10b、Wnt5a和β-catenin在BCC和SCC中均有表达，表达部位主要位于细胞浆和细胞核，在两种肿瘤中的阳性表达率均为100.0%；Wnt10a在BCC中未见阳性表达，而在SCC中阳性率为30.3%（10/33），差异有统计学意义（P<0.05）。结论　Wnt信号通路蛋白在BCC和SCC中均有明显表达，表明其在BCC的发生发展过程发挥了一定的作用。Wnt10a可以作为鉴别BCC和SCC的分子标志物。

关键词:

基底细胞癌, 鳞状细胞癌, Wnt, 免疫组织化学