Abstract:

Objective　To investigate the effect of edaravone on oxidative stress factors in renal tissue of rats with diquat (DQ) poisoning. Methods　Forty-two male SD rats were selected for the study, with a body weight of 250-300 g and an age of 8-10 weeks. During the experiment, 6 rats died, and the other ones were divided into a normal saline group, a DQ group, an edaravone group, and a DQ + edaravone group, with 9 in each group. The models of diquat poisoning were established by intraperitoneal injection of diquat in the rats, and the rats were intervened with edaravone. The expression levels of oxidative stress factors, superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) in the rats' kidney tissue were detected by the enzyme-linked immunosorbent assay. The infiltration of inflammatory cells in the rats was observed by the HE staining. All the experiments were repeated 3 times. The data were compared by the one-way analysis of variance. Results　In the normal saline group, the structures of glomeruli were normal; parts of the renal tubules were dilated; the renal interstitium was accompanied by a small amount of hemorrhage. In the DQ group, the renal tissue structure was disordered; the glomerulus and renal tubules were swollen and dilated; there was massive bleeding and infiltration of inflammatory cells in the renal interstitium. In the edaravone group, the renal tissue structure was normal; parts of the renal tubules were dilated; there was a small amount of hemorrhage in the glomeruli. In the DQ + edaravone group, the renal tissue structure was normal; parts of the renal tubules were dilated; there was a small amount of hemorrhage in the glomeruli; the infiltration of inflammatory cells was improved after the treatment. After DQ poisoning, the levels of SOD and CAT decreased, and the level of MDA increased [(27.160±1.540) U/mgprot, (3.320±0.052) U/mgprot, and (1.453±0.021) nmol/mgprot]. After the treatment with edaravone, the levels of SOD and CAT increased, and the level of MDA decreased [(35.100±2.241) U/mgprot, (4.112±0.687) U/mgprot, and (0.908±0.167) nmol/mgprot]. Conclusion　Edaravone can increase the expression of SOD and CAT and decrease the expression of MDA in kidney tissue of rats poisoned by diquat, playing a protective role in renal injury.

Key words:

Oxidative stress, Kidney injury, Diquat, Edaravone, Superoxide dismutase, Malondialdehyde, Catalase, Animal experiment, Rats

摘要：

目的　观察依达拉奉对敌草快急性中毒大鼠肾脏氧化应激因子的影响。方法　选取42只雄性SD大鼠，体重250～300 g，8～10周龄；实验过程中共有6只大鼠死亡，分为生理盐水组、敌草快组、依达拉奉组、敌草快+依达拉奉组，每组9只。向大鼠腹腔注射敌草快，建立敌草快中毒模型，采用依达拉奉进行腹腔注射干预。酶联免疫吸附试验法观察大鼠肾脏氧化应激因子［超氧化物歧化酶（SOD）、丙二醛（MDA）、过氧化氢酶（CAT）］的表达情况，HE染色观察大鼠炎性细胞浸润情况。所有实验均至少重复3次，组间两两比较采用单项方差分析。结果　生理盐水组肾小球结构正常，部分肾小管扩张，间质伴有少量出血；敌草快组肾组织结构紊乱，肾小球、肾小管肿胀扩张，间质伴有大量出血和炎性细胞浸润；依达拉奉组肾组织结构正常，部分肾小管扩张，肾小球伴有少量出血；敌草快+依达拉奉组相比敌草快组，肾组织结构恢复正常，部分肾小管扩张，肾小球伴有少量出血，炎性细胞浸润得到改善。敌草快中毒后，SOD和CAT水平显著下降，MDA水平则显著上升［（27.160±1.540）U/mgprot、（3.320±0.052）U/mgprot、（1.453±0.021）nmol/mgprot］；经依达拉奉治疗后，SOD和CAT水平上升，MDA水平显著下降［（35.100±2.241）U/mgprot、（4.112±0.687）U/mgprot、（0.908±0.167）nmol/mgprot］。结论　依达拉奉可提高敌草快中毒大鼠肾脏组织的SOD、CAT表达，降低MDA表达，对肾脏损伤起到保护作用。

关键词:

氧化应激, 肾损伤, 敌草快, 依达拉奉, 超氧化物歧化酶, 丙二醛, 过氧化氢酶, 动物实验, 大鼠