PIVKA-Ⅱ、DKK-4、lncRNA H19在卵巢癌中的表达及其与卵巢超声血流参数的相关性分析

doi:10.3760/cma.j.cn441417-20241219-16009

摘要/Abstract

摘要：

目的　分析维生素K缺乏或拮抗剂-Ⅱ诱导的蛋白质（PIVKA-Ⅱ）、Dickkopf-4（DKK-4）、长链非编码核糖核酸H19（lncRNA H19）在卵巢癌中的表达及其与卵巢超声血流参数的相关性。方法　采用前瞻性、单中心研究，纳入2019年4月至2024年1月在烟台市烟台山医院进行体检的95例健康女性（A组）及接受治疗的87例卵巢良性肿瘤患者（B组）、80例卵巢癌患者（C组）。A组年龄（54.73±6.93）岁，体重指数（21.16±0.63）kg/m²，合并高血压9例，糖尿病7例，高脂血症7例；B组年龄（55.49±7.72）岁，体重指数（21.09±0.72）kg/m²，合并高血压7例，糖尿病6例，高脂血症5例；C组年龄（54.90±6.84）岁，体重指数（20.95±0.68）kg/m²，合并高血压7例，糖尿病3例，高脂血症3例。比较３组血清PIVKA-Ⅱ、DKK-4、lncRNA H19水平及卵巢超声血流参数，采用Pearson相关系数分析血清PIVKA-Ⅱ、DKK-4、lncRNA H19水平与卵巢超声血流参数的相关性，受试者操作特征曲线（ROC）分析三者对卵巢癌的诊断价值，采用χ²检验或秩和检验、单因素方差分析和LSD-t检验进行统计分析。结果　C组、B组和A组血清PIVKA-Ⅱ、DKK-4、lncRNA H19水平及VM、PSV、EDV呈降低趋势（均P<0.05），RI、PI呈升高趋势（均P<0.05）。Pearson相关系数分析结果显示，血清PIVKA-Ⅱ、DKK-4、lncRNA H19与卵巢RI、PI呈负相关（r=-0.649、-0.518、-0.537、-0.519、-0.527、-0.618，均P<0.05），与VM、PSV、EDV呈正相关（r=0.722、0.509、0.599、0.611、0.694、0.531、0.548、0.704、0.605，均P<0.05）。ROC分析结果显示，血清PIVKA-Ⅱ、DKK-4、lncRNA H19联合诊断卵巢癌的曲线下面积高于单独检测（均P<0.05）。结论　卵巢癌患者血清PIVKA-Ⅱ、DKK-4、lncRNA H19水平升高，其与卵巢超声血流参数存在明显相关性，且三者联合检测可有效提高对卵巢癌的诊断价值。

关键词:

卵巢癌, 维生素K缺乏或拮抗剂-Ⅱ诱导的蛋白质, Dickkopf-4, 长链非编码核糖核酸H19, 超声, 血流参数, 相关性, 诊断价值

Abstract:

Objective　To analyze the protein induced by vitamin K absence or antagonist-Ⅱ (PIVKA-Ⅱ), Dickkopf-4 (DKK-4), and long-chain noncoding ribonucleic acid H19 (lncRNA H19) in ovarian cancer and their correlation with ovarian ultrasound blood flow parameters. Methods　From April 2019 to January 2024, 95 healthy women who underwent physical examination, 87 patients with benign ovarian tumors, and 80 patients with ovarian cancer were selected from Yantai Yantaishan Hospital as group A, group B, and group C, respectively. Group A were (54.73±6.93) years old; their body mass index was (21.16±0.63) kg/m²; there were 9 cases of hypertension, 7 cases of diabetes, and 7 cases of hyperlipidemia. Group B were (55.49±7.72) years old; their body mass index was (21.09±0.72) kg/m²; there were 7 cases of hypertension, 6 cases of diabetes, and 5 cases of hyperlipidemia. Group C was (54.90 ± 6.84) years old; their body mass index was (20.95±0.68) kg/m²; there were 7 cases of hypertension, 3 cases of diabetes, and 3 cases of hyperlipidemia. The serum levels of PIVKA-Ⅱ, DKK-4, and lncRNA H19 and ovarian ultrasound blood flow parameters were compared between the three groups. The Pearson correlation coefficient was used to analyze the correlation of the serum levels of PIVKA-Ⅱ, DKK-4, and lncRNA H19 with ovarian ultrasound blood flow parameters. The receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of the three for ovarian cancer. χ² test or rank sum test, one-way analysis of variance, and LSD-t test were used for the statistical analysis. Results　The serum levels of PIVKA-Ⅱ, DKK-4, and lncRNA H19, VM, PSV, and EDV in group C, group B and group A showed a decreasing trend (all P<0.05), while RI and PI showed an increasing trend (both P<0.05). The Pearson correlation coefficient analysis showed that serum PIVKA-Ⅱ, DKK-4, and lncRNA H19 were negatively correlated with ovarian RI and PI (r=-0.649, -0.518, -0.537, -0.519, -0.527, and -0.618; all P<0.05), and positively with VM, PSV, and EDV (r=0.722, 0.509, 0.599, 0.611, 0.694, 0.531, 0.548, 0.704, and 0.605; all P<0.05). The results of ROC analysis showed that the area under the curve of the combination of serum PIVKA-Ⅱ, DKK-4, and lncRNA H19 in the diagnosis of ovarian cancer was higher than that of each one alone (all P<0.05). Conclusions　The occurrence of ovarian cancer can increase the serum levels of PIVKA-Ⅱ, DKK-4, and lncRNA H19, which are correlated with the parameters of ovarian ultrasound blood flow. The combination of the three can effectively improve the diagnostic value of ovarian cancer.

Key words:

Ovarian cancer, Protein induced by vitamin K deficiency or antagonist-Ⅱ, Dickkopf-4, Long-chain noncoding ribonucleic acid H19, , Ultrasound, Blood flow parameters, Correlation, Diagnostic value

赵艳张天美姜淑燕王允芹宋修芹.

PIVKA-Ⅱ、DKK-4、lncRNA H19在卵巢癌中的表达及其与卵巢超声血流参数的相关性分析 [J]. 国际医药卫生导报, 2025, 31(16): 2683-2687.

Zhao Yan, Zhang Tianmei, Jiang Shuyan, Wang Yunqin, Song Xiuqin.

Expressions of PIVKA-Ⅱ, DKK-4, and lncRNA H19 in ovarian cancer and correlation with ovarian ultrasound blood flow parameters [J]. International Medicine and Health Guidance News, 2025, 31(16): 2683-2687.

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