基于PI3K/AKT信号通路探析归肾丸对薄型子宫内膜的治疗机制

doi:10.3760/cma.j.cn441417-20241021-16001

国际医药卫生导报 ›› 2025, Vol. 31 ›› Issue (16): 2641-2646.DOI: 10.3760/cma.j.cn441417-20241021-16001

• 妇产科专栏 • 下一篇

基于PI3K/AKT信号通路探析归肾丸对薄型子宫内膜的治疗机制

邱超¹ 刘燕² 关永格³ 宋阳⁴

¹长沙医学院基础医学院，长沙 410203；²长沙医学院中医学院，长沙 410203；³广州中医药大学第三附属医院妇二科，广州 510360；⁴广州中医药大学护理学院，广州 510080

收稿日期:2024-10-21 出版日期:2025-08-15 发布日期:2025-08-28
通讯作者: 刘燕，Email：937248762@qq.com
基金资助:
广东省科技计划（2017A020215119）；广东省普通高校特色创新项目（2017KTSCX041）

Investigation of mechanism of Guishen pills in treatment of thin endometrium based on PI3K/AKT signaling pathway

Qiu Chao¹, Liu Yan², Guan Yongge³, Song Yang⁴

¹Basic Meducal School, Changsha University, Changsha 410203, China; ²School of Traditional Chinese Medicine, Changsha University, Changsha 410203, China; ³Second Department of Gynecology, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510360, China; ⁴Nursing School, Guangzhou University of Chinese Medicine, Guangzhou 510080, China

Received:2024-10-21 Online:2025-08-15 Published:2025-08-28
Contact: Liu Yan, Email: 937248762@qq.com
Supported by:
Plan of Science and Technology in Guangdong Province (2017A020215119); Characteristic Innovation Project of Ordinary Universities in Guangdong Province (2017KTSCX041)

摘要/Abstract

摘要：

目的　探析归肾丸水提物治疗薄型子宫内膜的作用机制及与磷酸肌醇3-激酶（PI3K）/蛋白激酶B（AKT）信号通路的关系。方法　研究时间为2017年9月至2019年12月，从3周龄SD大鼠（3只，每只体重约50 g）的股骨中取得骨髓间充质干细胞（BMSCs），制备原代细胞模型。采用归肾丸水提物及雌激素进行体外诱导，Western Blotting检测各组BMSCs中PI3K、AKT、磷酸化PI3K（p-PI3K）、磷酸化AKT（p-AKT）蛋白的相对表达量，免疫荧光法检测各组白细胞抑制因子（LIF）、血管内皮生长因子（VEGF）的表达，酶联免疫吸附试验（ELISA）检测各组BMSCs上清中整合素αvβ3（ITGαvβ3）的表达。采用单因素方差分析、秩和检验进行统计比较。结果　归肾丸水提物干预组中的PI3K、p-PI3K、AKT、p-AKT的蛋白表达均高于空白对照组和雌激素组［0.69±0.03比0.56±0.02比0.56±0.05、0.55±0.01比0.43±0.04比0.45±0.02、0.52±0.06比0.42±0.00比0.35±0.02、0.32±0.05比0.26±0.02比0.18±0.01，均P<0.05］；空白对照组未见LIF、VEGF蛋白表达，雌激素组和归肾丸组均有LIF、VEGF表达，且归肾丸组比雌激素组的表达量更高；各组细胞上清中的ITGαvβ3表达水平比较，差异无统计学意义（P>0.05）。结论　雌激素和归肾丸均可通过调控PI3K/AKT信号通路，提升内膜血管生成因子，改善子宫内膜容受性并增加子宫内膜厚度，从而治疗薄型子宫内膜，且归肾丸水提物的诱导分化作用较雌激素单体强。

关键词:

薄型子宫内膜, 归肾丸, 磷酸肌醇3-激酶/蛋白激酶B信号通路, 治疗机制

Abstract:

Objective　To explore the mechanism of aqueous extract of Guishen pills in the treatment of thin endometrium, and its relationship with the phos-phatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathway. Methods　The study was from September 2017 to December 2019. The bone marrow mesenchymal stem cells (BMSCs) were taken from 3 weeks old SD rats (3 rats, each weighs approximately 50 grams) to prepare primary cell models. The aqueous extract of Guishen pills was used for in vitro induction. The relative expression levels of phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT), phosphorylated PI3K (p-PI3K), and phosphorylated AKT (p-AKT) in each group were detected by Western blotting. The expressions of leukocyte inhibition factor (LIF) and vascular endothelial growth factor (VEGF) were detected by the immunofluorescence method. The expression of integrin αvβ3 (ITGαvβ3) in the serum of BMSCs in each group was detected by enzyme linked immunosorbent assay. One-way analysis of variance and rank sum test were used for the statistical analysis. Results　The expressions of PI3K, p-PI3K, AKT, and p-AKT proteins in the aqueous extract intervention group were higher than those in the blank control group and the estrogen group (0.69±0.03 vs. 0.56±0.02 vs. 0.56±0.05, 0.55±0.01 vs. 0.43±0.04 vs. 0.45±0.02, 0.52±0.06 vs. 0.42±0.00 vs. 0.35±0.02, and 0.32±0.05 vs. 0.26±0.02 vs. 0.18±0.01; all P<0.05). No expression of LIF and VEGF proteins was observed in the blank control group. Both the estrogen group and the Guishen pill group showed expression of LIF and VEGF, and the expression levels in the Guishen pill group were higher than those in the estrogen group. There was no statistical difference in the expression level of ITGαvβ3 between the groups (P>0.05). Conclusions　Both Guishen pills and estrogen in the treatment of thin endometrium may be by regulating the PI3K/AKT signaling pathway, promoting angiogenesis factors, and improve the endometrial lining. The induction action of aqueous extract of Guishen pills is better than estrogen.

Key words:

Thin endometrium, Guishen , pills, Phos-phatidylinositol 3-kinase/protein kinase B signaling pathway, Therapeutic mechanism

邱超刘燕关永格宋阳.

基于PI3K/AKT信号通路探析归肾丸对薄型子宫内膜的治疗机制 [J]. 国际医药卫生导报, 2025, 31(16): 2641-2646.

Qiu Chao, Liu Yan, Guan Yongge, Song Yang.

Investigation of mechanism of Guishen pills in treatment of thin endometrium based on PI3K/AKT signaling pathway [J]. International Medicine and Health Guidance News, 2025, 31(16): 2641-2646.

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基于PI3K/AKT信号通路探析归肾丸对薄型子宫内膜的治疗机制

Investigation of mechanism of Guishen pills in treatment of thin endometrium based on PI3K/AKT signaling pathway

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