关键词:

肺结核, 结核杆菌, 菌型, 药物敏感性

Abstract:

Objective　To study the determination of mycobacterium tuberculosis types and drug sensitivity in patients with different pulmonary tuberculosis. Methods　From June 2019 to June 2021, 256 sputum samples of the patients with pulmonary tuberculosis were collected from Dongguan Songshanhu Central Hospital, including 114 females and 141 males who were 20-78 (46.59±4.67) years old. After isolation and culture, the mycobacterium tuberculosis type determination and drug sensitivity test were conducted to analyze the status of anti-tuberculosis drug resistance and single drug resistance in the patients with different tuberculosis. χ² test was applied. Results Two hundred and twenty-eight strains of Mycobacterium tuberculosis were isolated from the 256 sputum samples. According to the results of mycobacterium tuberculosis type determination, the human mycobacterium tuberculosis accounted for the highest proportion, 82.46%(188/228). Other types of bacteria accounted for the lowest proportion, 2.19%(5/228). The drug sensitivity test showed that among the 256 cases of tuberculosis, 153 cases were resistant to the first-line anti-tuberculosis drugs, accounting for 59.77%; the drug resistance rate against isoniazid was the highest [22.27% (57/256)]; the drug resistance rate against ethambutol was the lowest [6.25% (16/256)]. Thirty cases were resistant to the second-line anti-tuberculosis drugs, accounting for 11.72%; the drug resistance rate against ofloxacin was the highest [4.69% (12/256)]; the drug resistance rate against Amikacin sulfate was the lowest [1.95% (5/256)]. The drug sensitivity test showed that among the 256 cases of tuberculosis, 76 cases had single drug resistance against the first-line anti-tuberculosis drugs, accounting for 29.69%; the single drug resistance rate against isoniazid was the highest [10.16% (26/256)]; the single drug resistance rate against ethambutol was the lowest [5.47% (14/256)]. Fifteen cases had single drug resistance against the second-line anti-tuberculosis drugs, accounting for 5.86%; the single drug resistance rate against ofloxacin was the highest [3.13% (8/256)]; the single drug resistance rate against Amikacin sulfate was the lowest [0.39% (1/256)]. Conclusions　Human mycobacterium tuberculosis is the main form of mycobacterium tuberculosis in patients with tuberculosis. The drug resistance of tuberculosis patients is relatively serious, and their drug resistance to first-line anti-tuberculosis drugs is higher. The clinical resistance monitoring of anti-tuberculosis drugs should be strengthened.

Key words:

Pulmonary tuberculosis, Mycobacterium tuberculosis, Bacteria types, Sensitivity to drugs