关键词:

肝细胞癌, HBV, T淋巴细胞, 壳酶蛋白, DR-70

Abstract:

Objective　To investigate the changes and clinical significance of the levels of the T-cell subsets (CD3⁺, CD3⁺CD4⁺, and CD3⁺CD8⁺), fibrin degradation complex (DR-70), and chitinase 3-like protein 1 (CHI3H1) in patients with different clinical classification of hepatitis B virus (HBV) infections, and to analyze the correlation of T lymphocyte subsets with CHI3H1 and DR-70 in patients with HBV related hepatocellular carcinoma. Methods　One hundred and seventy-three patients with chronic HBV infection treated at Qishan Hospital were selected as an experimental group, including 114 males and 59 females who were (45.38±12.72) years old. According to their conditions, there were 59 HBV carriers, 51 cases of chronic hepatitis B, 31 cases of liver cirrhosis, and 32 cases of hepatocellular carcinoma. Forty healthy examinees during the same period were selected as a control group, including 22 males and 18 females who were (39.15±14.95) years old. The various T-cell subsets were measured by flow cytometry. The peripheral blood DR-70 levels were quantitatively detected using the double antibody sandwich enzyme-linked immunosorbent assay. The magnetic particle chemiluminescence method was used to detect the peripheral blood CHI3H1 level. The Welch analysis of variance was used for the comparison of normally distributed continuous data with heterogeneity of variance between the groups. The Games-Howell test was used for further the comparison between two the groups.The Kruskal-Wallis H test was used for the comparison of the non-normally distributed continuous data between the groups.The χ² test was used for the comparison of the categorical data between the groups. The Pearson correlation analysis was used to investigate the correlation. Results　The CD3⁺ level in the hepatocellular carcinoma group was the lowest [(61.43±19.26)%], and the CD3⁺CD4⁺ level in the liver cirrhosis group was the lowest, with statistical differences from those in the control group (both P<0.05). The levels of DR-70 and CHI3H1 in the chronic hepatitis B group, the liver cirrhosis group, and the hepatocellular carcinoma group increased in different degrees (all P<0.01), and those in the hepatocellular carcinoma group were the highest [(30.11±9.96) mg/L and (213.11±39.76) μg/L; both P<0.01]. The proportion of CD3⁺ T lymphocytes in the peripheral blood in the hepatocellular carcinoma group was negatively correlated with the DR-70 level (r=-0.291, P=0.037). Conclusions　The serum levels of DR-70 and CHI3L1 are high in patients with hepatocellular carcinoma. Their combination with T lymphocyte subsets can evaluate the progression of patients with chronic liver diseases and especially has some value in the early diagnosis of liver tumor.

Key words:

Hepatocellular carcinoma, Hepatitis B virus, T lymphocyte subsets, Chitinase 3-like protein 1, DR-70