解毒消癥饮调控β-catenin转位抑制人Huh7和PLC/PRF/5细胞株侧群细胞增殖的机制

doi:10.3760/cma.j.issn.1007-1245.2023.18.013

国际医药卫生导报 ›› 2023, Vol. 29 ›› Issue (18): 2571-2575.DOI: 10.3760/cma.j.issn.1007-1245.2023.18.013

解毒消癥饮调控β-catenin转位抑制人Huh7和PLC/PRF/5细胞株侧群细胞增殖的机制

杨瑞明^1,2 薛易³ 江芷珊³ 曹治云^1,2 陈旭征^1,2

¹福建中医药大学中西医结合研究院，福州　350122；²福建省中西医结合老年性疾病重点实验室，福州　350122；³福建中医药大学药学院，福州　350122

收稿日期:2023-07-18 出版日期:2023-09-15 发布日期:2023-09-22
通讯作者: 陈旭征，Email：471235814@qq.com
基金资助:
福建省自然科学基金项目（2020J01747）；陈可冀中西医结合发展基金（CKJ2022006）

Mechanism regulating β-catenin translocation by Jiedu Xiaozheng Yin to inhibit proliferation of side population cells in human Huh7 and PLC/PRF/5 cell lines

Yang Ruiming^1,2, Xue Yi³, Jiang Zhishan³, Cao Zhiyun^1,2, Chen Xuzheng^1,2

¹Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; ²Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China; ³School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China

Received:2023-07-18 Online:2023-09-15 Published:2023-09-22
Contact: Chen Xuzheng, Email: 471235814@qq.com
Supported by:
Project of Fujian Natural Science Foundation (2020J01747); Chen Keji Fund for Development of Integrative Medicine (CKJ2022006)

摘要/Abstract

摘要：

目的　从肝癌侧群（side population，SP）细胞的角度探讨解毒消癥饮（Jiedu Xiaozheng Yin，JXY）乙酸乙酯提取物（ethyl acetate extract from JXY，EE-JXY）抑制肝癌细胞生长的作用机制。方法　研究时间2023年1月至5月。体外培养人肝癌细胞株Huh7和PLC/PRF/5，流式细胞仪分析肝癌细胞中SP细胞的含量并分选SP细胞；平板克隆实验检测SP细胞的克隆形成能力；克隆球形成实验检测SP细胞的体外成球能力；免疫细胞荧光染色技术检测EE-JXY对肝癌SP细胞β-catenin蛋白转位的影响。结果　EE-JXY组SP细胞的含量显著低于空白组；SP细胞克隆形成能力和体外成球能力均受到显著抑制；EE-JXY使部分SP细胞β-catenin的表达从胞浆和细胞核转位到了细胞膜。结论　EE-JXY能显著抑制Huh7和PLC/PRF/5肝癌SP细胞的增殖，其机制可能与其抑制β-catenin的转位有关。

关键词:

肝癌, 解毒消癥饮, 侧群细胞, β-catenin

Abstract:

Objective　To investigate the mechanism by which ethyl acetate extract from Jiedu Xiaozheng Yin (EE-JXY) inhibits the growth of hepatocellular carcinoma cells, specifically focusing on side population (SP) cells in hepatocellular carcinoma. Methods　This study was from January to May 2023. Human Huh7 and PLC/PRF/5 cell lines were cultured in vitro. The presence of SP cells in the Huh7 and PLC/PRF/5 cell lines was determined and subsequently isolated by the flow cytometry. The colony formation ability of the SP cells was detected by the plate cloning assay. The SP cells' ability to form spheres in vitro was evaluated by the clonal sphere-forming assay. The effect of EE-JXY on the β-catenin protein translocation in the SP cells of hepatocellular carcinoma was detected by immunofluorescence. Results　The SP cells' quantity in the EE-JXY group was significantly lower than that in the blank group. The EE-JXY group showed a significant inhibition in the clonal formation and pellet formation abilities of the SP cells in vitro. The results of immunofluorescence showed that the β-catenin in the cytoplasm and nucleus was translocated to the cell membrane in some SP cells by EE-JXY. Conclusions　EE-JXY can significantly inhibit the proliferation of SP cells in Huh7 and PLC/PRF/5 cell lines; the mechanism may be related to the inhibition of β-catenin translocation.

Key words:

Hepatocellular carcinoma, Jiedu Xiaozheng Yin, Side population cells, β-catenin

杨瑞明薛易江芷珊曹治云陈旭征.

解毒消癥饮调控β-catenin转位抑制人Huh7和PLC/PRF/5细胞株侧群细胞增殖的机制 [J]. 国际医药卫生导报, 2023, 29(18): 2571-2575.

Yang Ruiming, Xue Yi, Jiang Zhishan, Cao Zhiyun, Chen Xuzheng.

Mechanism regulating β-catenin translocation by Jiedu Xiaozheng Yin to inhibit proliferation of side population cells in human Huh7 and PLC/PRF/5 cell lines [J]. International Medicine and Health Guidance News, 2023, 29(18): 2571-2575.

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解毒消癥饮调控β-catenin转位抑制人Huh7和PLC/PRF/5细胞株侧群细胞增殖的机制

Mechanism regulating β-catenin translocation by Jiedu Xiaozheng Yin to inhibit proliferation of side population cells in human Huh7 and PLC/PRF/5 cell lines

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