关键词: 网络药理学, 筋痛灵膏, 肩关节周围炎

Abstract: Objective　To study the potential mechanism of the main active ingredients of Jintongling ointment in the treatment of periarthritis of shoulder by network pharmacology. Methods　The main active components and targets of Jintongling ointment were obtained from Traditional Chinese Medicine System Pharmacology Database and Analysis Platform (TCMSP), and the targets of periarthritis of shoulder were obtained searching the five disease databases. The drug-disease interaction targets were obtained from the Venny platform, and the protein interaction network was established to screen the core targets. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed on the Metascape platform. Finally, molecular docking was performed on the drug's main components and core targets. Results　Fifty-three active components and 121 important targets were screened out; among them, the core active components were quercetin, β-sitosterol, sitosterol, and baicalein, and the key targets were JUN, ESR1, and TP53. GO functional enrichment analysis showed that the biological pathways of Jintongling ointment included the responses to exogenous stimuli and bacterial source molecules, wound healing, and the response to oxygen level. The KEGG pathway enrichment analysis showed that the treatment of periarthritis of shoulder with Jintongling ointment was mainly enriched in interleukin-17 signaling pathway, tumor necrosis factor signaling pathway, AGE-RAGE signaling pathway in diabetic complications, etc. Conclusion　Jintongling ointment regulates JUN, ESR1, and TP53 key targets through core components and acts on a variety of biological pathways to participate in the regulation of immune response and reduce inflammatory response, so as to achieve the effect of treating periarthritis of shoulder.

Key words: Network pharmacology, Jintongling ointment, Periarthritis of shoulder