siRNA干扰VNUT基因对INS-1胰岛素分泌的影响

doi:10.3760/cma.j.issn.1007-1245.2022.04.016

国际医药卫生导报 ›› 2022, Vol. 28 ›› Issue (3): 360-362.DOI: 10.3760/cma.j.issn.1007-1245.2022.04.016

siRNA干扰VNUT基因对INS-1胰岛素分泌的影响

彭淑莹王泽骑陈立强

肇庆医学高等专科学校，肇庆　526060

收稿日期:2021-09-22 出版日期:2022-02-01 发布日期:2022-03-01
通讯作者: 陈立强，Email：lqchen81@163.com
基金资助:
肇庆市科技创新计划项目（202004031441）；

肇庆医学高等专科学校校级教研项目（2019K28）；

肇庆医专学生科技活动基金项目（2021SX03）

Effects of siRNA interfering with VNUT gene on insulin secretion in INS-1

Peng Shuying, Wang Zeqi, Chen Liqiang

Zhaoqing Medical Collage, Zhaoqing 526060, China

Received:2021-09-22 Online:2022-02-01 Published:2022-03-01
Contact: Chen Liqiang, Email: lqchen81@163.com
Supported by:
肇庆市科技创新计划项目（202004031441）；

肇庆医学高等专科学校校级教研项目（2019K28）；

肇庆医专学生科技活动基金项目（2021SX03）

摘要/Abstract

摘要： 目的　观察小干扰RNA（small interfering RNA，siRNA）干扰囊泡膜核苷酸转运体（vesicular nucleotide transporter，VNUT）基因对大鼠胰腺β细胞（INS-1）胰岛素分泌的影响。方法　2021年1月至6月通过逆转录-聚合酶链反应（RT-PCR）、蛋白印迹和酶联免疫吸附剂测定（ELISA）等方法检测siRNA对VNUT基因表达的作用以及对胰岛素分泌的影响。采用独立样本t检验，P<0.05为差异有统计学意义。结果　siRNA转染INS-1细胞24 h后，VNUT基因表达明显下降，对照组和干扰组RNA表达结果分别为（0.452±0.218）和（0.319±0.116），蛋白分别为（0.514±0.297）和（0.369±0.203），差异均有统计学意义（均P<0.05）；胰岛素分泌明显抑制，对照组和干扰组结果为（281.45±24.92）ng/L和（176.54±15.70）ng/L，差异有统计学意义（P<0.05）。结论　siRNA明显抑制VNUT基因表达与INS-1胰岛素分泌，深入研究VNUT基因对胰岛素分泌的影响可为2型糖尿病发病机制因素提供参考资料。

关键词: siRNA, 2型糖尿病, 胰岛素分泌, 囊泡膜核苷酸转运体

Abstract: Objective　To investigate the effect of small interfering RNA (siRNA) on the expression of vesicular nucleotide transporter (VNUT) gene and insulin secretion in rat pancreatic β-tumor cells (INS-1). Methods　From January to June 2021, the effects of siRNA on VNUT gene expression and insulin secretion were detected by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and enzyme linked immunosorbent assay (ELISA). The independent-sample t test was applied. When P<0.05, there is a statistical difference. Results　Twenty-four hours after siRNA was transfect into the INS-1, in the control group and the interfering group, the levels of RNA were (0.452±0.218) and (0.319±0.116), and the levels of proteins were (0.514±0.297) and (0.369±0.203), with statistical differences between the two groups (both P<0.05); the levels of insulin secretion were (281.45±24.92) ng/L and (176.54±15.70) ng/L in the control group and the interfering group, respectively, with a statistical difference between the two groups (P<0.05). Conclusion　siRNA obviously inhibits VNUT gene expression and insulin secretion in INS-1, and that deeply studying the effect of VNUT gene on insulin secretion can provide references for the pathogenesis of type 2 diabetes.

Key words: siRNA, Type 2 diabetes, Insulin secretion, Vesicular nucleotide transporter

彭淑莹, 王泽骑, 陈立强.

siRNA干扰VNUT基因对INS-1胰岛素分泌的影响 [J]. 国际医药卫生导报, 2022, 28(3): 360-362.

Peng Shuying, Wang Zeqi, Chen Liqiang. Effects of siRNA interfering with VNUT gene on insulin secretion in INS-1[J]. International Medicine and Health Guidance News, 2022, 28(3): 360-362.

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siRNA干扰VNUT基因对INS-1胰岛素分泌的影响

Effects of siRNA interfering with VNUT gene on insulin secretion in INS-1

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