近海浅海海水弗朗西斯菌的分离培养和鉴定

doi:10.3760/cma.j.issn.1007-1245.2022.03.014

国际医药卫生导报 ›› 2022, Vol. 28 ›› Issue (3): 353-357.DOI: 10.3760/cma.j.issn.1007-1245.2022.03.014

近海浅海海水弗朗西斯菌的分离培养和鉴定

陈富¹ 李敏² 冯俊慧³ 罗海敏³ 李良慧³ 廖焕兰⁴ 屈平华⁴ 李松⁴

¹广州市番禺区健康管理中心，广州　511490； ²中山大学肿瘤防治中心，广州　510006； ³广州中医药大学第二临床医学院，广州　510006； ⁴广州中医药大学第二附属医院　广东省中医院检验医学部，广州　510006

收稿日期:2021-10-19 出版日期:2022-02-01 发布日期:2022-03-01
通讯作者: 廖焕兰，Email：13560085390@163.com
基金资助:
广东省自然基金（2020A1515011119）；

广东省教育厅“创新强校工程”科研项目特色创新类项目（2018KTSCX042）；

广东省中医药管理局科研项目（20191133）

**Isolation, cultivation, and identification of Francisella from near-shore shallow coastal areas**

Chen Fu¹, Li Min², Feng Junhui³, Luo Haimin³, Li Lianghui³, Liao Huanlan⁴, Qu Pinghua⁴, Li Song⁴

¹Panyu Health Management Center, Guangzhou 511490, China;
²Cancer Center, Sun Yat-Sen University, Guangzhou 510006, China;
³The Second Clinic Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, China;
⁴Department of Clinical Laboratory, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, China

Received:2021-10-19 Online:2022-02-01 Published:2022-03-01
Contact: Liao Huanlan, Email: 13560085390@163.com
Supported by:
Guangdong Nature Fund (2020A1515011119)；

Special Innovation Project of Scientific Research Program of "Program for Innovation and High Quality Schools" of Guangdong Education Department (2018KTSCX042)；

Scientific Research Project of Guangdong Bureau of Chinese Medicine Administration (20191133)

摘要/Abstract

摘要： 目的　对广东地区近海浅海海水弗朗西斯菌进行分离培养，以了解弗朗西斯菌在近海浅海海水中的分布情况。方法　在广东近海浅海地区水域内，选取茂名市电白区水东湾（2019年10月4日）以及惠州市两大湾（巽寮湾、双月湾，2019年8月12日）进行多点采集水样，分别19份和29份。利用前期探索出针对分离弗朗西斯菌的有效海水前处理手段（浓缩、酸处理）以及分离培养基（BHI-2216E-GVPC和BCYEα-2216E-GVPC）进行分离培养，依据弗朗西斯菌的菌落特征，初步将可疑菌株进行分纯培养，并对纯培养后的可疑菌株进行传统形态特征、生理生化特征、16S rRNA分子特征等鉴定。结果　19份水东湾水样中有14份（阳性率为73.7%）成功分离到共60株目标菌（含邻近兼性胞内寄生菌）；29份惠州地区水样中有17份（阳性率为58.6%）成功分离到共79株目标菌（含邻近兼性胞内寄生菌）。经菌落特征、细菌形态学检查、生化反应以及测序结果比对后，水东地区60株目标菌分离出弗朗西斯菌属（含潜在新种）12株（20.0%），潜在新科2株（2.9%），惠州地区79株目标菌分离出弗朗西斯菌属（含潜在新种）22株（27.8%），潜在新科18株（22.8%）。结论　广东地区近海浅海海水中弗朗西斯菌菌种资源丰富，且存在不同的该菌属内新种以及可能存在邻近新属。

关键词: 弗朗西斯菌, 分离培养, 鉴定

Abstract:

Objective　To investigate the distribution of Francisella from near-shore shallow coastal areas in Guangdong, China by analyzing the seawater samples from various sources. Methods　Nineteen and 29 water samples were collected from the near-shore shallow coastal areas at Shuidong Bay at Dianbai District, Maoming City (on October 4, 2019) and Xunliao Bay and Double-Moon Bay in Huizhou City (on August 12, 2019) in Guangdong, China. The early exploratory outcomes of the optimal pretreatment method (concentrate and acid treatment) and culture medium (BHI-2216E-GVPC and BCYEα-2216E-GVPC) were applied to isolate Francisella. The colonies were selected based on Francisella colony morphology and the selected single colonies were cultured separately on plates to get the pure culture for further study, such as traditional morphological features, biochemical/physiological features, and 16S rRNA gene sequencing identification. Results　Fourteen of the 19 samples were positive (73.7%) and were collected from Shuidong Bay, and a total of 60 strains (including facultative intracellular bacteria) were isolated. Seventeen of the 29 samples were positive (58.6%), and were collected from Huizhou City, and a total of 79 strains (including facultative intracellular bacteria) were isolated. We examined the morphological, biochemical and 16S rRNA gene features of all the isolated strains. Among the 60 strains from Shuidong Bay, 12 strains (20.0%, including potential new species) were identified into Francisella, and 2 strains belonged to a potential new family (2.9%). Among the 79 strains from Huizhou City, 22 strains (27.8%, including potential new species) were identified into Francisella, and 18 strains (22.8%) belonged to a potential new family. Conclusion　The Francisella resources, including potential new species, and its neighboring potential new family are abundant in near-shore shallow coastal areas in Guangdong, China.

Key words: Francisella, Isolation and cultivation, Identification

陈富, 李敏, 冯俊慧, 罗海敏, 李良慧, 廖焕兰, 屈平华, 李松. 近海浅海海水弗朗西斯菌的分离培养和鉴定[J]. 国际医药卫生导报, 2022, 28(3): 353-357.

Chen Fu, Li Min, Feng Junhui, Luo Haimin, Li Lianghui, Liao Huanlan, Qu Pinghua, Li Song. Isolation, cultivation, and identification of Francisella from near-shore shallow coastal areas[J]. International Medicine and Health Guidance News, 2022, 28(3): 353-357.

参考文献

[1] Kreitmann L, Terriou L, Launay D, et al. Disseminated Infection Caused by Francisella philomiragia, France, 2014[J]. Emerg Infect Dis, 2015,21(12):2260-2261. DOI: 10.3201/eid2112.150615.

[2] 张磊,郑敏玲,王亚,等.基于高通量测序的平均核苷酸一致性在1株弗朗西斯菌鉴定中的应用[J].临床检验杂志,2017,35(7):499-502.DOI:10.13602/j.cnki.jcls.2017.07.05.

[3] 张磊,叶大柠,朱焱,等.10株蜃楼弗朗西斯菌的鉴定与特征分析[J].临床检验杂志,2017,35(4):271-276.DOI:10.13602/j.cnki.jcls.2017.04.09.

[4] 马丹娟,黄研,卢春敏,等.2016—2018年某医院产超广谱β-内酰胺酶肺炎克雷伯菌的临床分布及耐药性分析[J].国际医药卫生导报,2020,26(12):1672-1675.DOI:10.3760/cma.j.issn.1007-1245.2020.12.007.

[5] Qu PH, Li Y, Salam N, et al. Allofrancisella inopinata gen. nov., sp. nov. and Allofrancisella frigidaquae sp. nov, isolated from water-cooling systems, and transfer of Francisella guangzhouensis Quet al. 2013 to the new genus as Allofrancisella guangzhouensis comb. Nov[J]. Int J Syst Evol Microbiol, 2016,66(11):4832-4838. DOI: 10.1099/ijsem.0.001437.

[6] Zheng ML, Jiao JY, Dong L, et al. Pseudofrancisella aestuarii gen. nov., sp. nov., a novel member of the family Francisellaceae isolated from estuarine seawater[J]. Antonie Van Leeuwenhoek, 2019,112(6):877-886. DOI: 10.1007/s10482-018-01220-0.

[7] Liu L, Salam N, Jiao JY, et al. Cysteiniphilum litorale gen. nov., sp. nov., isolated from coastal seawater[J]. Int J Syst Evol Microbiol, 2017,67(7):2178-2183. DOI: 10.1099/ijsem.0.001917.

[8] Xiao M, Salam N, Liu L, et al. Fastidiosibacter lacustris gen. nov., sp. nov., isolated from a lake water sample, and proposal of Fastidiosibacteraceae fam. nov. within the order Thiotrichales[J]. Int J Syst Evol Microbiol, 2018,68(1):347-352. DOI: 10.1099/ijsem.0.002510.

[9] Xiao M, Zheng ML, Salam N, et al. Facilibium subflavum gen. nov., sp. nov. and Cysteiniphilum halobium sp. nov., new members of the family Fastidiosibacteraceae isolated from coastal seawater[J]. Int J Syst Evol Microbiol, 2019,69(12):3757-3764. DOI: 10.1099/ijsem.0.003676.

[10] Wu HY, Yan H, Zheng ML, et al. Legionella qingyii sp. nov., isolated from water samples in China[J]. Int J Syst Evol Microbiol, 2019,69(7):2017-2022. DOI: 10.1099/ijsem.0.003421.

近海浅海海水弗朗西斯菌的分离培养和鉴定

**Isolation, cultivation, and identification of Francisella from near-shore shallow coastal areas**

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