关键词:

甲状腺癌, 赤芍总苷, circ_HOMER1, 增殖, 迁移, 侵袭, miR-23a-3p

Abstract:

Objective　To investigate the effect and mechanism of total peony glycosides (TPG) on the malignant behaviors of thyroid cancer cells (SW579). Methods　The cancer tissue and their adjacent tissue of 90 patients with thyroid cancer who were diagnosed and received surgical treatment in Xi'an Workers Hospital from June 2016 to December 2019 were collected. The circ_HOMER1 expression level in the thyroid cancer tissue was detected by the real-time fluorescent quantitative reverse transcription PCR (RT-qPCR); the relationship between circ_HOMER1 expression and clinicopathological characteristics of the patients was explored. The SW579 cells were divided into a control (con) group, a TPG 50 mg/L group, a TPG 100 mg/L group, a TPG 200 mg/L group, an si-NC group, an si-circ_HOMER1 group, a TPG + anti-miR-23a-3p group, and a TPG + pcDNA-circ_HOMER1 group. The RT-qPCR was performed to detect the expressions of circ_HOMER1 and miR-23a-3p; the Transwell method was employed to detect the cell migration and invasion; the plate cloning and CCK-8 assays were used to detect the cell proliferation. The targeting relationship between circ_HOMER1 and miR-23a-3p was determined by the dual luciferase reporter assay. The correlation between circ_HOMER1 expression and clinicopathological features was evaluated. χ² test and one-way analysis of variance were used for the statistical analysis. Results　The expression of circ_HOMER1 was up-regulated in the thyroid cancer tissue. The circ_HOMER1 expression was correlated with TNM stage, lymph node metastasis, and envelope infiltration (all P<0.05). The expression levels of circ_HOMER1 in the TPG 50 mg/L group, the TPG 100 mg/L group, and the TPG 200 mg/L group were lower than that in the control group (0.78±0.04, 0.47±0.03, and 0.23±0.02 vs. 1.00±0), while the levels of miR-23a-3p were higher (1.49±0.03, 2.11±0.06, and 2.89±0.09 vs. 1.00±0), with statistical differences (F=1 425.931 and 1 904.071; both P<0.05). The numbers of migration, clone, and invasion in in the TPG 50 mg/L group, the TPG 100 mg/L group, and the TPG 200 mg/L group were lower than those in the control group, while the cell inhibition rates were higher (all P<0.05). The migration, proliferation, and invasion behaviors of the SW579 cells in the si-circ_HOMER1 group were lower than those in the si-NC group (all P<0.05). The proliferation, migration, and invasion behaviors in the TPG+pcDNA-circ_HOMER1 group and the TPG+anti-miR-23a-3p group were higher than those in the TPG group (all P<0.05). MiR-23a-3p was a target gene of circ_HOMER1. Conclusion　TPG may inhibit the malignant proliferation, migration, and invasion of thyroid cancer cells down-regulating the circ_HOMER1/miR-23a-3p axis.

Key words:

Thyroid cancer, Total paeony glycosides, Circ_HOMER1, Proliferation, Migration, Invasion, MiR-23a-3p