关键词:

胃癌, 同源结构域相互作用蛋白激酶2, 多药耐药基因1, 免疫组织化学

Abstract:

Objective　To detect the expressions of homodomain-interacting protein kinase-2 (HIPK2) and multiple drug resistance-1 (MDR-1) in gastric cancer tissue, and to explore their clinical significance. Methods　One hundred and fifty-six cases of gastric cancer tissue surgically resected in Binzhou Medical University Hospital from August 1, 2011 to October 1, 2015 were collected, including 99 males and 57 females who were 36-79 years old, with a median age of 56. The immunohistochemical method was used to detect the expressions of HIPK2 and MDR-1. The relationship between HIPK2 expression and clinicopathological parameters and the correlation with MDR-1 expression were analyzed. χ² test was used for the comparison between the groups; Pearson correlation analysis was used for the correlation analysis between the genes. Results　The immunohistochemistry results showed that the positive expression rates of HIPK2 and MDR-1 in the gastric cancer tissue were 29.5% (46/156) and 46.8% (73/156), respectively. There were no statistical differences in the HIPK2 positive-expression rate between the patients with different Lauren types, tumor sites, lymph-vessel invasion, lymph node metastasis, and genders (all P>0.05). The HIPK2 positive-expression rates in the patients with maximum tumor diameter≥6.5 cm and positive MDR-1 expression were lower than those in the patients with maximum tumor diameter<6.5 cm and negative MDR-1 expression (both P<0.05). HIPK2 expression was negatively correlated with MDR-1 expression (r=-0.167; P<0.05). Conclusion　HIPK2 is lowly expressed in gastric cancer tissue and negatively correlated with the expression of MDR-1, and may be involved in the processes of gastric cancer development and progression.

Key words:

Gastric cancer, Homeodomain-interacting protein kinase 2, Multiple drug resistance-1, Immunohistochemistry