Abstract: Objective　To analyze the correlations between changes of serum related cytokines and clinical stage in patients with primary glaucoma. Methods　A total of 85 patients with primary glaucoma treated in Zhengzhou Second People's Hospital from July 2019 to March 2021 were selected as a study group [40 males and 45 females, aged (56.67±2.12) years], and 50 healthy subjects who underwent physical examination in the hospital during the same period were selected as a control group [23 males and 27 females, aged (57.17±2.33) years]. The levels of interleukin-4 (IL-4), IL-6, and IL-12 were compared between the two groups. According to the clinical symptoms and visual field examination results, the patients in the study group were divided into a mild stage group (29 cases), a moderate stage group (33 cases), and a severe stage group (23 cases). The levels of IL-4, IL-6, and IL-12 in patients with mild stage, moderate stage, and severe stage were compared and analyzed. Logistic regression analysis was used to explore the correlations between the levels of IL-4, IL-6, and IL-12 and the clinical stage in patients with primary glaucoma, and Pearson correlation analysis was used to analyze the correlations between the changes of serum related cytokines and the clinical stage in patients with primary glaucoma. Independent sample t test was used for comparison of the measurement data between two groups, F test was used for comparison of the measurement data among multiple groups, and χ² test was used for the count data. Results　The level of IL-4 in the study group was (236.89±45.36) pg/ml, which was significantly higher than that in the control group [(191.65±50.34) pg/ml], and the levels of IL-6 and IL-12 were (35.42±28.46) pg/ml and (96.94±51.23) pg/ml, respectively, which were significantly lower than those in the control group [(61.18±30.21) pg/ml and (130.21±56.55) pg/ml], with statistically significant differences (all P<0.05). There were no statistically significant differences in the gender, age, blood pressure, and blood glucose among patients with mild stage, moderate stage, and severe stage (all P>0.05); the intraocular pressure (IOP) in the three groups became higher and higher from mild to severe stage, with a statistically significant difference among the three groups (P<0.05). The serum levels of IL-6 and IL-12 in the three groups decreased significantly from mild to severe stage, and the levels of IL-6 and IL-12 in patients with severe stage were significantly lower than those in patients with mild stage (both P<0.05); the serum level of IL-4 in the three groups increased gradually from mild to severe stage, but there was no statistically significant difference among the three groups (P>0.05). Logistic regression analysis showed that IL-6 and IL-12 levels were significantly correlated with the clinical stage of primary glaucoma (both P<0.05); Pearson analysis showed that the changes of serum IL-6 and IL-12 levels were negatively correlated with the clinical stage of primary glaucoma patients (r=-0.450, -0.430). Conclusion　The changes of serum related cytokines have a certain correlation with the clinical stage of primary glaucoma, which can be used to evaluate the severity of primary glaucoma and provide scientific basis for clinical treatment.

Key words: Primary glaucoma, Clinical stage, Serum related cytokines, Correlation

摘要： 目的　分析血清相关细胞因子水平变化与原发性青光眼患者临床分期的相关性。方法　选取2019年7月至2021年3月郑州市第二人民医院收治的85例原发性青光眼患者作为研究组，其中男40例，女45例，年龄（56.67±2.12）岁；另选取同期于医院进行体检的50例健康者作为对照组，其中男23例，女27例，年龄（57.17±2.33）岁。对比两组白细胞介素（IL）-4、IL-6、IL-12水平。根据研究组患者临床症状及视野检查结果将患者分为轻症期（29例）、中症期（33例）和重症期（23例），对比分析3组患者IL-4、IL-6、IL-12水平，采用logistic回归分析探讨IL-4、IL-6、IL-12水平与原发性青光眼患者临床分期的关联性，采用Pearson相关性分析血清相关细胞因子水平变化与原发性青光眼患者临床分期的相关性。计量资料两组间比较采用独立样本t检验，多组间比较采用F检验，计数资料采用χ²检验。结果　研究组IL-4水平为（236.89±45.36）pg/ml，明显高于对照组（191.65±50.34）pg/ml，IL-6、IL-12水平分别为（35.42±28.46）pg/ml、（96.94±51.23）pg/ml，均明显低于对照组（61.18±30.21）pg/ml、（130.21±56.55）pg/ml，差异均有统计学意义（均P<0.05）。轻症组、中症组和重症组患者性别、年龄、血压、血糖比较，差异均无统计学意义（均P>0.05）；临床分期由轻症到重症患者眼压越来越高，3组间比较差异有统计学意义（P<0.05）。3组临床分期由轻症到重症患者血清IL-6、IL-12水平下降明显，且重症组患者IL-6、IL-12水平均显著低于轻症组（均P<0.05）；3组临床分期由轻到重症患者血清IL-4水平逐渐增加，但组间比较差异无统计学意义（P>0.05）。logistic回归分析结果显示IL-6、IL-12水平变化与原发性青光眼患者临床分期有显著关系（均P<0.05）；经Pearson分析结果显示，血清IL-6与IL-12水平变化与原发性青光眼患者临床分期呈负相关（r=-0.450、-0.430）。结论　血清相关细胞因子水平变化与原发性青光眼临床分期具有一定相关性，可用于评估原发性青光眼患者病情严重程度，为临床治疗提供科学依据。

关键词: 原发性青光眼, 临床分期, 血清相关细胞因子, 相关性