Abstract:

Objective　To explore the values of the low ionic coacervate technique and card microcolumn gel technique for clinical blood transfusion testing of newborns with ABO hemolytic disease. Methods　Seventy-six newborns who developed ABO hemolytic disease and were admitted to Xianning Central Hospital between May 2019 and March 2022 were prospectively and randomly selected for this study, including 43 males and 38 females who were (2.85±0.95) d old. All the newborns were clinically tested for blood transfusion using the low ionic coacervate technique and card microcolumn gel technique. The final neonatal treatment outcomes were set as the gold standard. The success rates, test efficacies, and testing times of the transfusion testing techniques in the two groups were compared by χ² and t tests. Results　Among the 76 neonates, 69 cases were successfully treated, and the treatment success rate was 90.79%; 7 cases failed, and the treatment failure rate was 9.21%. The blood coordination success rate by the low ionic coacervate technique was not statistically different from that by the card microcolumn gel technique [85.53% (65/76) vs. 94.70% (71/76); P>0.05]. The sensitivity, specificity, and accuracy of the low ionic coacervate technique were not statistically different from those of the card microcolumn gel technique [85.51% (62/69) vs. 98.55% (68/69), 57.14% (4/7) vs. 57.14% (4/7), and 85.52% (66/76) vs. 92.11% (72/76); all P>0.05]. The examination time of the low ionic coacervate technique was shorter than that of the card microcolumn gel technique [(16.11±1.79) min vs. (17.43±1.94) min], with a statistical difference (P<0.05). Conclusion　There are no significant differences in the success rate of blood matching and test efficacy between the low ionic coacer vate technique and the cartridge microcolumn gel technique in the transfusion test of clinical neonatal ABO hemolytic disease, but the test time of the low ion polyamine technique is relatively shorter.

Key words:

Newborns, ABO hemolytic disease, Low ionic coacervate technique, Card microcolumn gel test technique

摘要：

目的　探讨ABO新生儿溶血病临床输血检验应用低离子凝聚胺技术与卡式微柱凝胶试验技术的价值。方法　前瞻性随机选取咸宁市中心医院2019年5月至2022年3月间收治的发生ABO溶血病新生儿76例，男婴43例，女婴38例，出生天数为（2.85±0.95）d。均使用低离子凝聚胺技术、卡式微柱凝胶试验技术进行输血检验，以新生儿最终治疗结果为金标准。比较两组输血检验技术的配血成功率、检验效能、检验时间，组间比较采用χ²、t检验。结果　76例新生儿中，实际成功治疗69例，治疗成功率为90.79%，失败7例，治疗失败率为9.21%。低离子凝聚胺技术配血成功率为85.53%（65/76），卡式微柱凝胶试验技术的配血成功率为94.70%（71/76），差异无统计学意义（P>0.05）。低离子凝聚胺技术检验灵敏度为85.51%（62/69）、特异度为57.14%（4/7）、准确度为85.52%（66/76），卡式微柱凝胶试验技术的灵敏度为98.55%（68/69）、特异度为57.14%（4/7）、准确度为92.11%（72/76），差异均无统计学意义（均P>0.05）。低离子凝聚胺技术检验时间为（16.11±1.79）min，卡式微柱凝胶试验技术检验时间为（17.43±1.94）min，两者比较，差异有统计学意义（P<0.05）。结论　低离子凝聚胺技术和卡式微柱凝胶试验技术用于临床新生儿ABO溶血病的输血检验中配血成功率及检验效能无明显差异，但低离子凝聚胺技术检验时间较短。

关键词:

新生儿, ABO溶血病, 低离子凝聚胺技术, 卡式微柱凝胶试验技术