Abstract:

Objective　To explore the value of the levels of miR-29c-3p, miR-143-5p, and miR-205-5p in the prediction of the clinical prognosis of patients with nasopharyngeal carcinoma. Methods　This was a prospective study. One hundred and twenty-six patients with nasopharyngeal carcinoma diagnosised and treated at Second Hospital, Air Force Medical University from January 2019 to April 2022 were selected as an observation group, including 90 males and 36 females who were (47.65 7.97) years old. Fifty healthy examinees during the same period were selected as a control group, including 31 males and 19 females who were (45.38±8.53) years old. The expression levels of miR-29c-3p, miR-143-5p, and miR-205-5p in the biopsy tissues from both groups were measured by reverse transcription polymerase chain reaction (RT-PCR), and their correlation with the patients' clinical prognosis was explored. The survival curve was drawn by the Kaplan-Meier method, the Log-Rank test was used. The measurement data of normal distribution were compared between the groups by t test, and the count data by χ² test. Results　The levels of miR-29c-3p and miR-143-5p in the observation group were lower than those in the control group (2.68±0.64 vs. 5.86±1.95 and 1.83±0.49 vs. 5.04±1.67); the level of miR-205-5p in the observation group was higher than that in the control group (4.81±1.68 vs. 3.89±1.08); there were statistical differences (t=16.284, 19.623, and 4.302; all P<0.05). The levels of miR-29c-3p, miR-143-5p, and miR-205-5p were not correlated with gender, age, T stage, lymph node metastasis, distant metastasis, and clinical stage (all P>0.05). The Kaplan-Meier survival curves showed that the 2-year overall survival rate in the high-expression group of miR-205-5p was lower than that in the low-expression group of miR-205-5p [76.19% (48/63) vs. 90.48% (57/63)], with a statistical difference (χ²=4.629; P<0.05). The 2-year overall survival rates in the high-expression groups of miR-29c-3p and miR-143-5p were higher than those in the low-expression groups of miR-29c-3p and miR-143-5p [93.65% (59/63) vs. 73.02% (46/63) and 95.24% (60/63) vs. 71.43% (45/63)], with statistical differences (χ²=9.657 and 12.857; both P<0.05). Conclusions　The expressions of miR-29c-3p and miR-143-5p are reduced, while the expression of miR-205-5p is increased in patients with nasopharyngeal carcinoma. These expression patterns are significantly correlated with patient survival rates and can serve as prognostic biomarkers.

Key words:

Nasopharyngeal carcinoma, Mir-29C-3P, Mir-143-5P, Mir-205-5p, Clinical prognosis

摘要：

目的　探讨miR-29c-3p、miR-143-5p及miR-205-5p水平对鼻咽癌患者临床预后的预测价值。方法　采用前瞻性研究，选取2019年1月至2022年4月在空军军医大学第二附属医院耳鼻咽喉头颈外科诊治的126例鼻咽癌患者作为观察组，同期选取50例健康体检者作为对照组。观察组男90例、女36例，年龄（47.65±7.97）岁；对照组男31例、女19例，年龄（45.38±8.53）岁。通过逆转录聚合酶链反应（RT-PCR）法检测鼻咽癌患者及对照组活检组织中miR-29c-3p、miR-143-5p及miR-205-5p的表达水平，并探讨其表达水平与鼻咽癌临床预后之间的关系。采用Kaplan-Meier法绘制生存曲线，并行Log-Rank检验。正态分布的计量资料组间比较采用t检验，计数资料组间比较行χ² 检验。结果　观察组miR-29c-3p、miR-143-5p水平分别为2.68±0.64、1.83±0.49，均低于对照组的5.86±1.95、5.04±1.67，miR-205-5p水平为4.81±1.68，高于对照组的3.89±1.08，差异均有统计学意义（t=16.284、19.623、4.302，均P<0.05）。miR-29c-3p、miR-143-5p、miR-205-5p水平与性别、年龄、T分期、淋巴结转移、远处转移及临床分期无明显相关性（均P>0.05）。Kaplan-Meier生存曲线结果显示，miR-205-5p高表达组患者2年累积生存率为76.19%（48/63），显著低于低表达组患者的90.48%（57/63），差异有统计学意义（χ²=4.629，P<0.05）；miR-29c-3p、miR-143-5p高表达组患者2年总生存率均高于低表达组患者［93.65%（59/63）比73.02%（46/63）、95.24%（60/63）比71.43%（45/63）］，差异均有统计学意义（χ²=9.657、12.857，均P<0.05）。结论　miR-29c-3p及miR-143-5p在鼻咽癌患者中表达降低，miR-205-5p表达升高，这些表达模式与患者生存率显著相关，可作为预后生物标志物。

关键词:

鼻咽癌, miR-29c-3p, miR-143-5p, miR-205-5p, 临床预后