Abstract:

Objective　To explore the role and potential mechanism of polypyrimidine tract binding protein 3 (PTBP3) in esophageal squamous cell carcinoma. Methods　Six specimens of esophageal squamous cell carcinoma and six specimens of its adjacent normal tissue were collected from Jining First People's Hospital from January 2021 to May 2023. The expressions of PTBP3 in the tissue and the cells were determined by real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting; the CCK8 assay kit was used to determine the ESCC cells' viability; the scratch experiment was used to determine the ESCC cells' migration ability. The UALCAN and TCGA databases were searched to analyze the expression difference of PTBP3 between the esophageal cancer tissue and the normal esophageal tissue, and the relationship between PTBP3 and miR29 family. t test was used. Results　The expression of PTBP3 in the ESCC tissue was higher than that in the normal esophageal tissue, with a statistical difference (P<0.05). The results of qRT-PCR showed that the expressions of miR-29a-3p and miR-29c-3p in the PTBP3 knocked down group were lower than those in the control virus group in the 3 ESCC cells (KYSE30, ECA109, and KYSE150)(t=29.007, 7.057, 4.844, 12.625, 15.612, and 18.861; all P<0.05). In the ESCC tissue, the expressions of miR-29a-3p and miR-29c-3p were correlated with the expression of PTBP3. Conclusion　PTBP3 may be an important molecular marker and potential therapeutic target for ESCC. It can provide new ideas and a method for the early diagnosis and a treatment of ESCC.

Key words:

Esophageal cancer, Squamous cell carcinoma, PTBP3, Tumor metastasis

摘要：

目的　探讨多嘧啶束结合蛋白3（PTBP3）在食管鳞状细胞癌（ESCC）中的作用及其潜在机制。方法　收集2021年1月至2023年5月济宁市第一人民医院的6例ESCC标本及其癌旁正常组织标本，采用实时荧光定量聚合酶链式反应（qRT-PCR）与蛋白质印迹（Western blotting）测定组织及细胞中PTBP3的表达；细胞计数试剂盒（CCK8）测定ESCC细胞活力情况；划痕试验测定ESCC细胞迁移能力。通过检索UALCAN数据库、癌症基因组图谱（TCGA）数据库分析食管癌组织及正常食管组织PTBP3的表达差异及PTBP3与miR29家族的关系。统计学方法采用t检验。结果　ESCC组织中PTBP3基因表达量高于正常食管组织（P<0.05）。qRT-PCR结果表明，与对照病毒组相比，3种ESCC细胞（KYSE30、ECA109、KYSE150）的PTBP3敲低组中miR-29a-3p与miR-29c-3p表达水平均下调（t=29.007、7.057、4.844、12.625、15.612、18.861，均P<0.05）。ESCC组织中miR-29a-3p和miR-29c-3p表达量与PTBP3表达量相关。结论　PTBP3是ESCC的一个重要分子标志物和潜在治疗靶点，可为ESCC早期诊断及治疗提供新思路和方法。

关键词:

食管癌, 鳞状细胞癌, PTBP3, 肿瘤转移