Abstract:

Objective　To study the relationship between β-galactoside α-2, 6-sialyltransferase 1 (ST6Gal1) and the clinicopathological characteristics and prognosis of patients colorectal cancer (CRC). Methods　A total of 102 patients with CRC who underwent surgical resection and had tumor and adjacent tissues preserved in Department of Pathology, Shaanxi Nuclear Industry 215 Hospital from December 2018 to June 2023 were selected. The positive rates of ST6Gal1 in the tumor tissues of the patients with different clinicopathological characteristics were compared. The patients were followed up, and their prognosis was recorded. The survival patients were set as a survival group, and the dead patients a death group. There were 37 males and 25 females in the survival group; there were 48 patients who were ≥60 years old and 25 patients who were <60 years old. In the death group, there were 15 males and 16 females; there were 25 cases who were ≥60 years old and 6 cases who were <60 years old. The prognostic factors of the patients with CRC were analyzed by the COX regression model. Kaplan-Meier curves were plotted to compare the prognosis of the patients with positive and negative expression of ST6Gal1, and Log-rank test was performed. Results　The positive rate of ST6Gal1 in the CRC tissue was higher than that in the paracancer tissue [67.62% (79/102) vs. 13.33% (12/102); χ²=58.417, P<0.001]. Compared with those in the patients with CRC TNM stage Ⅰ-Ⅱ [50.00% (17/34)], adenocarcinoma [71.79% (56/78)], highly differentiated CRC [69.84% (44/63)], no serous infiltration [60.00% (21/35)], and no lymph node metastasis [62.50% (30/48)], the positive rates of ST6Gal1 were higher in the patients with TNM stage Ⅲ-Ⅳ [91.18% (62/68)], mucinous adenocarcinoma/sig-ring cell carcinoma [95.83% (23/24)], serous infiltration [86.57% (58/67)], medium/low differentiation [89.74% (35/39)], and lymph node metastasis [90.74% (49/54)] (χ²=22.006, 6.072, 5.463, 9.291, and 11.605; P<0.001, =0.014, =0.019, =0.002, and =0.001). The COX regression model showed that TNM stage Ⅲ-Ⅳ, serosal invasion, tumor diameter ≥5 cm, moderate/low differentiation, lymph node metastasis, and ST6Gal1 positivity were risk factors for poor prognosis of the patients with CRC (all P<0.05). Log-rank test showed that there was a statistical difference in the Kaplan-Meier curves between the patients with positive and negative expressions of ST6Gal1 (χ²=8.578; P=0.003). Conclusion　ST6Gal1 is abnormally highly expressed in CRC tissue, is related to various clinicopathological characteristics, and is one of the risk factors for poor prognosis of patients with CRC.

Key words:

Colorectal cancer, β-galactoside α-2, 6-sialyltransferase 1, Clinicopathology, Prognosis

摘要：

目的　研究β半乳糖苷α-2，6-唾液酸转移酶1（ST6Gal1）与结直肠癌（CRC）患者临床病理特征及预后的关系。方法　选取2018年12月至2023年6月陕西省核工业二一五医院病理科保存的102例CRC患者术后切除组织及癌旁组织切片。比较不同临床病理特征CRC患者癌组织ST6Gal1阳性率；随访并统计患者预后，将生存患者设为生存组，病死患者设为死亡组；生存组男37例，女25例；年龄≥60岁48例，<60岁25例。死亡组男15例，女16例；年龄≥60岁25例，<60岁6例。采用COX回归模型分析CRC患者预后影响因素；绘制Kaplan-Meier曲线，比较ST6Gal1阳性、阴性表达患者预后，行Log-rank检验。结果　CRC组织ST6Gal1阳性率高于癌旁组织［67.62%（79/102）比13.33%（12/102）；χ²=58.417，P<0.001］。与CRC TNM分期Ⅰ～Ⅱ期［50.00%（17/34）］、腺癌［71.79%（56/78）］、高分化［69.84%（44/63）］、无浆膜浸润［60.00%（21/35）］、无淋巴结转移［62.50%（30/48）］患者比较，CRC TNM分期Ⅲ～Ⅳ期［91.18%（62/68）］、黏液腺癌/印戒细胞癌［95.83%（23/24）］、浆膜浸润［86.57%（58/67）］、中/低分化［89.74%（35/39）］、有淋巴结转移［90.74%（49/54）］患者ST6Gal1阳性率较高（χ²=22.006、6.072、5.463、9.291、11.605，P<0.001、=0.014、=0.019、=0.002、=0.001）。COX回归模型显示，TNM Ⅲ～Ⅳ期、浆膜浸润、肿瘤长径≥5 cm、中/低分化、淋巴结转移及ST6Gal1阳性是CRC患者预后不良的危险因素（均P<0.05）。Log-rank检验显示，ST6Gal1阳性、阴性表达患者Kaplan-Meier曲线差异有统计学意义（χ²=8.578，P=0.003）。结论　CRC组织中ST6Gal1异常高表达，与多种临床病理特征相关，且是CRC患者预后不良的危险因素之一。

关键词:

结直肠癌, β半乳糖苷α-2, 6-唾液酸转移酶1, 临床病理, 预后