Abstract:

Objective　To analyze the effect of Zhisanzhen acutherapy on learning and memory abilities of rats with post-stroke cognitive impairment (PSCI). Methods　The animal experimental research was conducted from August 2 to October 3, 2022. Forty-five SPF SD male rats were randomly divided into a sham-operation group, a PSCI control group, and a Zhisanzhen group, with 15 rats in each group. The rat model of PSCI was established by middle cerebral artery occlusion (MCAO) method. Morris water maze test was used to determine the differences in learning and memory abilities in rats. The rats in the three groups were intervened for 21 days. The escape latency and swimming distance of rats in the three groups were tested by Morris water maze test after intervention. Analysis of variance was used for comparison among multiple groups, LSD-t test was used for pairwise comparison between groups, and paired t test was used for self-control within groups. Results　Finally, 12 rats survived in the Zhisanzhen group, 11 in the PSCI control group, and 12 in the sham-operation group. After modeling/sham-operation, there were statistically significant differences in the escape latency [(51.94±8.19) s, (53.13±6.28) s, and (41.66±6.65) s] and swimming distance [(1 242.25±100.72) cm, (1 192.28±137.72) cm, and (702.92±85.60) cm] among the three groups (F=4.060 and 4.240, both P<0.05). Compared with the sham-operation group, the escape latency and swimming distance of the rats in the PSCI control group and the Zhisanzhen group were significantly extended, with statistically significant differences (t=2.228, 2.219, 2.304, and 2.343, all P<0.05). Compared with the PSCI control group, there were no statistically significant differences in the escape latency or swimming distance in the Zhisanzhen group (t=1.620 and 1.866, both P>0.05). Compared to those before modeling, the escape latency [(51.94±8.19) s vs. (37.32±6.19) s, (53.13±6.28) s vs. (35.90±6.08) s] and swimming distance [(1 242.25±100.72) cm vs. (623.52±42.22) cm, (1 192.28±137.72) cm vs. (629.36±39.87) cm] of the Zhisanzhen group and the PSCI control group after modeling were increased, with statistically significant differences (t=2.713, 2.692, 2.208, and 2.276, all P<0.05). Compared to those before sham-operation, there were no statistically significant differences in the escape latency or swimming distance of the rats after sham-operation (t=1.478 and 1.372, both P>0.05). After acupuncture intervention, the escape latency of the rats in the Zhisanzhen group was significantly shortened compared with that before acupuncture intervention [(45.64±7.70) s vs. (51.94±8.19) s], and the swimming distance was significantly reduced [(887.35±92.47) cm vs. (1 242.25±100.72) cm], with statistically significant differences (t=2.245 and 2.211, both P<0.05). After acupuncture intervention, the escape latency and swimming distance of the rats in the PSCI control group and the sham-operation group were not significantly different from those before intervention (t=1.602, 1.370, 1.681, and 1.824, all P>0.05). After acupuncture intervention, there were statistically significant differences in the escape latency [(45.64±7.70) s, (56.33±10.32) s, and (39.17±5.35) s] and swimming distance [(887.35±92.47) cm, (1 292.29±104.52) cm, and (621.33±56.41) cm] among the three groups (F=4.270 and 4.320, both P<0.05). Compared with the sham-operation group, the escape latency and swimming distance of the rats in the PSCI control group and the Zhisanzhen group were extended, with statistically significant differences (t=2.508, 2.482, 2.473, and 2.318, all P<0.05). Compared with the PSCI control group, the escape latency and swimming distance of the rats in the Zhisanzhen group were decreased, with statistically significant differences (t=2.261 and 2.201, both P<0.05). Conclusion　Zhisanzhen acutherapy may improve the learning and memory abilities of PSCI rats.

Key words:

Zhisanzhen acutherapy, Acupuncture and moxibustion, Cognitive impairment, Stroke, Animal experiment

摘要：

目的　观察智三针疗法对脑卒中后认知功能障碍（PSCI）大鼠学习和记忆能力的影响。方法　2022年8月2日至10月3日进行动物实验研究。将45只SPF级SD雄性大鼠随机分为假手术组、PSCI大鼠对照组、智三针疗法组，每组15只。用大脑中动脉栓塞（MCAO）法制备PSCI大鼠模型，用Morris水迷宫实验检测大鼠的学习和记忆能力，分别给予3组大鼠相应干预21 d。用Morris水迷宫实验测定3组大鼠干预后的逃避潜伏期及游泳距离。计量资料多组间比较用方差分析，组间两两比较用LSD-t检验，组内自身对照用配对t检验。结果　最终智三针疗法组大鼠存活12只、PSCI大鼠对照组11只、假手术组12只。造模后/假手术后，3组大鼠逃避潜伏期［（51.94±8.19）s、（53.13±6.28）s、（41.66±6.65）s］及游泳距离［（1 242.25±100.72）cm、（1 192.28±137.72）cm、（702.92±85.60）cm］差异均有统计学意义（F=4.060、4.240，均P<0.05）；与假手术组比较，PSCI大鼠对照组、智三针疗法组大鼠的逃避潜伏期、游泳距离均明显延长，差异均有统计学意义（t=2.228、2.219、2.304、2.343，均P<0.05）；与PSCI大鼠对照组比较，智三针疗法组大鼠的逃避潜伏期、游泳距离差异均无统计学意义（t=1.620、1.866，均P>0.05）。与造模前比较，造模后智三针疗法组、PSCI大鼠对照组的逃避潜伏期［（51.94±8.19）s比（37.32±6.19）s、（53.13±6.28）s比（35.90±6.08）s］及游泳距离［（1 242.25±100.72）cm比（623.52±42.22）cm、（1 192.28±137.72）cm比（629.36±39.87）cm］均有增长，差异均有统计学意义（t=2.713、2.692、2.208、2.276，均P<0.05）；与假手术前比较，假手术后大鼠的逃避潜伏期、游泳距离差异均无统计学意义（t=1.478、1.372，均P>0.05）。针灸干预后，智三针疗法组大鼠逃避潜伏期与针灸干预前比较明显缩短［（45.64±7.70）s比（51.94±8.19）s］，游泳距离明显减少［（887.35±92.47）cm比（1 242.25±100.72）cm］，差异均有统计学意义（t=2.245、2.211，均P<0.05）；针灸干预后，PSCI大鼠对照组、假手术组大鼠的逃避潜伏期、游泳距离与干预前比较，差异均无统计学意义（t=1.602、1.370、1.681、1.824，均P>0.05）。针灸干预结束后，3组大鼠的逃避潜伏期［（45.64±7.70）s、（56.33±10.32）s、（39.17±5.35）s］及游泳距离［（887.35±92.47）cm、（1 292.29±104.52）cm、（621.33±56.41）cm］差异均有统计学意义（F=4.270、4.320，均P<0.05）；与假手术组比较，PSCI大鼠对照组、智三针疗法组大鼠的逃避潜伏期、游泳距离均延长，差异均有统计学意义（t=2.508、2.482、2.473、2.318，均P<0.05）；与PSCI大鼠对照组比较，智三针疗法组大鼠的逃避潜伏期缩短，游泳距离明显减少，差异均有统计学意义（t=2.261、2.201，均P<0.05）。结论　大鼠在卒中后认知障碍状态下，经过智三针疗法干预，在学习能力及记忆力方面都有改善。

关键词:

智三针, 针灸, 认知功能障碍, 脑卒中, 动物实验