Effects of cell block section and liquid-based smear on immunocytochemical staining of cytological samples

doi:10.3760/cma.j.issn.1007-1245.2023.18.021

International Medicine and Health Guidance News ›› 2023, Vol. 29 ›› Issue (18): 2606-2610.DOI: 10.3760/cma.j.issn.1007-1245.2023.18.021

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Effects of cell block section and liquid-based smear on immunocytochemical staining of cytological samples

Jiang Lijuan¹, Tang Futing², Jing Hongxia³

¹Department of Pathology, Women's Hospital of Nanjing Medical University, Nanjing Maternal and Child Health Care Hospital, Nanjing 210004, China; ²Department of Pathology, Hospital Affiliated to Nanjing University of Chinese Medicine, Jiangsu Hospital of Chinese Medicine, Nanjing 210029, China; ³Department of Pathology, Jiangning Hospital, Nanjing Medical University, Nanjing 210000, China

Received:2023-03-16 Online:2023-09-15 Published:2023-09-25
Contact: Tang Futing, Email: 493313495@qq.com
Supported by:
Project of Developmental Fund of Science and Technology of Nanjing Medical University (NMUB20210149)

细胞蜡块与液基制片技术对细胞学样本ICC染色的比较研究

蒋丽娟¹ 唐福婷² 景红霞³

¹南京医科大学附属妇产医院南京市妇幼保健院病理科，南京　210004；²南京中医药大学附属医院江苏省中医院病理科，南京　210029；³南京医科大学附属江宁医院病理科，南京　210000

通讯作者: 唐福婷，Email：493313495@qq.com
基金资助:
南京医科大学科学科技发展基金项目（NMUB20210149）

Abstract

Abstract:

Objective　To investigate the differences of immunocytochemistry results between cell block section and liquid-based smear of cytological samples. Methods　Fifty samples of pleural fluid diagnosed as lung adenocarcinoma in Jiangsu Hospital of Chinese Medicine from January to December 2022 were retrospectively analyzed. The immunocytochemistry staining of four antibodies to cytokeratin 7, epithelial specific antigen (MOC-31), aspartic protease A (Napsin-A), and thyroid transcription factor-1 (TTF-1) was performed on the cell block sections and liquid based smears, respectively. The positive expression rates and staining intensities of the four kinds of antibodies in the tumor cells on the cell block sections and liquid-based smears were compared. χ² test or fisher exact probability method were used. Results　There were statistical differences in the strong positive expression rates of MOC-31, Napsin-A, and TTF-1 between the cell block sections and liquid smears [80.0% (40/50) vs. 100.0% (50/50), 66.7% (28/42) vs. 88.9%(40/45), and 76.7% (33/43) vs. 95.7%(44/46); χ²=11.111, 6.285, and 6.811; all P<0.05]. Conclusion　Both the cell block and liquid-based smear have good immunocytochemical effects on different antibodies in serosal cavity effusion samples, but the liquid-based smear can improve the intensity of immunocytochemical staining. When the cell block staining is not effective, the liquid-based smear is recommended for immunocytochemical staining.

Key words:

Immunocytochemistry, Cell block, Liquid-based smear

摘要：

目的　探讨细胞学样本细胞蜡块与液基制片技术对不同抗体免疫细胞化学（ICC）染色的差异。方法　回顾性分析江苏省中医院2022年1月至12月的50例诊断为肺腺癌的癌性胸水样本，在细胞蜡块切片与液基涂片上分别行角蛋白7（CK7）、上皮特异性抗原（MOC-31）、天冬氨酸蛋白酶A（Napsin-A）及甲状腺转录因子（TTF-1）4个抗体的ICC染色，比较4种抗体在细胞蜡块切片与液基涂片上肿瘤细胞的免疫染色阳性表达率与染色强度的差异，采用χ²检验或Fisher确切概率法。结果　MOC-31、Napsin-A及TTF-1的细胞蜡块切片与液基涂片强阳性表达率比较［80.0%（40/50）比100.0%（50/50）、66.7%（28/42）比88.9%（40/45）、76.7%（33/43）比95.7%（44/46）］，差异均有统计学意义（χ²=11.111、6.285、6.811，均P<0.05）。结论　细胞蜡块及液基制片技术对浆膜腔积液样本的不同抗体的ICC效果均较好，但液基涂片能提高浆膜腔积液样本ICC染色的强度，染色质量更佳，细胞蜡块染色效果欠佳时，推荐用液基涂片行ICC染色。

关键词:

免疫细胞化学, 液基制片, 细胞蜡块

Jiang Lijuan, Tang Futing, Jing Hongxia.

Effects of cell block section and liquid-based smear on immunocytochemical staining of cytological samples [J]. International Medicine and Health Guidance News, 2023, 29(18): 2606-2610.

蒋丽娟唐福婷景红霞.

细胞蜡块与液基制片技术对细胞学样本ICC染色的比较研究 [J]. 国际医药卫生导报, 2023, 29(18): 2606-2610.

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Effects of cell block section and liquid-based smear on immunocytochemical staining of cytological samples

细胞蜡块与液基制片技术对细胞学样本ICC染色的比较研究

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