Abstract:

Objective　To investigate the expressions of small ubiquitin related modifier (SUMO) specific protease 1 (SENP1) and estrogen (ER) in endometrioid adenocarcinoma and their relationship. Methods　Eighty samples of endometrioid adenocarcinoma tissue and 80 samples of normal endometrial tissue that were surgically removed in Luoyang Central Hospital from October 2018 to October 2020 were collected; the expressions of SENP1 and ER were detected by immunohistochemistry. The expressions of SENP1 and ER in the normal endometrial epithelial cells and the endometrial carcinoma cells (ishikawa) were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. The ishikawa cell lines were divided into two groups: one group used ER specific inhibitor (fulvestrant) to silence the expression of ER; the other group served as the control group; the expressions of SENP1 were observed. Pearson correlation coefficient was used for the correlation analysis. Results　The expressions of SENP1 and ER in the endometrioid adenocarcinoma tissue were higher than those in the normal endometrial tissue; their expressions were positively correlated (r=0.6). The expressions of SENP1 and ER in the endometrial carcinoma cells were higher than those in the normal endometrial epithelial cells. After silencing the expression of ER in the ishikawa cell line, the expression of SENP1 decreased, and the expressions were positively correlated (r=0.5). Conclusions　SENP1 and ER are highly expressed in endometrioid adenocarcinoma; they are positively correlated with each other; together they promote the occurrence and development of endometrial carcinoma.

Key words:

Endometrioid adenocarcinoma, SENP1, ER

摘要：

目的　探讨小泛素相关修饰物（SUMO）特异性蛋白酶1（SUMO specific protease 1，SENP1）和雌激素（ER）在子宫内膜样腺癌中的表达情况和两者之间的关系。方法　收集2018年10月至2020年10月在洛阳市中心医院行手术切除的子宫内膜样腺癌组织和正常子宫内膜组织各80份，采用免疫组化方法分别检测SENP1和ER的表达情况。采用逆转录聚合酶链式反应（RT-PCR）和蛋白质免疫印迹法（Western blotting）检测正常子宫内膜上皮细胞和子宫内膜癌细胞（ishikawa）中SENP1和ER的表达。将ishikawa细胞株分成两组，一组采用ER特异性抑制剂（氟维司群）沉默ER的表达，另一组作为对照组，分别观察SENP1的表达情况。相关分析采用Pearson相关系数。结果　与正常子宫内膜组织相比，SENP1和ER在子宫内膜样腺癌组织中高表达，并且表达呈正相关（r=0.6）。子宫内膜癌细胞SENP1和ER表达明显高于正常子宫内膜上皮细胞。在ishikawa中沉默ER的表达后，SENP1的表达随之降低，表达呈正相关（r=0.5）。结论　SENP1和ER在子宫内膜样腺癌中均高表达，并且二者存在一定的正相关性，共同促进子宫内膜癌的发生发展。

关键词:

子宫内膜样腺癌, SENP1, ER