Abstract:

Objective　The biological reference intervals of epstein-barr virus capsid antigen antibody (EBV-VCA-IgA) and epstein-barr virus early antigen antibody (EBV-EA-IgA) in our laboratory were established by an indirect method using the data from the laboratory information management system (LIS) of Guangdong Second Traditional Chinese Medicine Hospital. Methods　The results of EBV-VCA-IgA and EBV-EA-IgA in 2 160 healthy people (998 males and 1 162 females) who were 20 to 100 years old with normal aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (T-BILI), direct bilirubin (D-BILI), indirect bilirubin (I-BILI), creatinine (CR), and urea nitrogen (BUN) were retrospectively analyzed by the LIS data. The results were grouped by gender and age. Kolmogorov Smirnov test (K-S test) was used to test the normality of each group. When P>0.05, it was regarded as normal distribution; when P<0.05, it was regarded as non-normal distribution. For non-normal distribution data, the data larger than P₂₅-3×IQR and less than P₇₅+3×IQR were excluded. The non-parametric test was used to analyze the data after elimination. The statistical differences between the groups were analyzed. The biological reference intervals of EBV-VCA-IgA and EBV-EA-IgA were established by the P_2.5-P_97.5 intervals, and the reference intervals were verified. Results　There was a statistical difference in the EBV-VCA-IgA reference interval between the males and the females (P<0.001), but no statistical difference between the age groups (P=0.087). There was no statistical difference in the EBV-EA-IgA reference interval between the males and the females (P=0.127), but was between the age groups (P=0.004). The EBV-EA-IgA reference intervals in the 20-50 years old group and the ≥50-70 years old group were statistically different from that in the ≥70 years old group (P=0.002 and <0.001). There was no statistical difference in the EBV-EA-IgA reference interval between the 20-50 years old group and the ≥50-70 years old group (P=0.043), so these two groups were united as one group (20-70 years old) to build a reference interval, and it was statistically different from that in the ≥70 years old group (P=0.001). Accordingly, the EBV-VCA-IgA reference interval was 0.34-3.88 AU/ml in the males, and was 0.32-3.71 AU/ml in the females; the EBV- EA-IgA reference interval was 0.01-1.39 AU/ml in the 20-70 years old group, and was 0.01-1.52 AU/ml in the ≥70 years old group. Conclusion　The indirect method was used to establish the biological reference intervals of EBV-VCA-IgA and EBV-EA-IgA in our laboratory, which could provide more accurate results for the clinical diagnosis of diseases caused by EB virus, such as nasopharyngeal carcinoma.

Key words:

Epstein-barr virus, Indirect method, Biological reference interval, Epstein-barr virus capsid antigen antibody, Epstein-barr virus early antigen antibody

摘要：

目的　采用广东省第二中医院实验室信息管理系统（LIS）中的数据，以间接法来建立本实验室EB病毒衣壳抗原IgA抗体（EBV-VCA-IgA）和EB病毒早期抗原IgA抗体（EBV-EA-IgA）的生物参考区间。方法　对2020年8月至2021年7月广东省第二中医院LIS数据进行回顾性分析，选取2 160名（男998名，女1 162名）20～100岁且血清天冬氨酸转氨酶（AST）、血清丙氨酸转氨酶（ALT）、总胆红素（T-BILI）、直接胆红素（D-BILI）、间接胆红素（I-BILI）、血清肌酐（CR）及尿素氮（BUN）结果正常的健康体检人群的EBV-VCA-IgA和EBV-EA-IgA检测结果，对检测结果进行性别与年龄分组。使用Kolmogorov Smirnov（K-S）检验对各组别进行正态性检验，P>0.05视为正态分布，P<0.05视为非正态分布。对于非正态分布数据，剔除大于P₂₅-3×IQR及小于P₇₅+3×IQR数据。剔除后的数据进行非参数检验，分析各组间的差异。利用P_2.5～P_97.5区间来建立EBV-VCA-IgA及EBV-EA-IgA的生物参考区间，并对参考区间进行验证。结果　不同性别间的EBV-VCA-IgA参考区间差异有统计学意义（P<0.001），不同年龄组间的EBV-VCA-IgA差异无统计学意义（P=0.087）。EBV-EA-IgA不同性别间差异无统计学意义（P=0.127），不同年龄组间差异有统计学意义（P=0.004）。其中，EBV-EA-IgA 20～50岁组和≥50～70岁组分别与≥70岁组比较差异有统计学意义（P=0.002，P<0.001），EBV-EA-IgA 20～50岁组和≥50～70岁组比较差异没有统计学意义（P=0.043），合并为1组（20～70岁）建立参考区间，且与≥70岁组比较差异有统计学意义（P=0.001）。因此，EBV-VCA-IgA参考区间：男性为0.34～3.88 AU/ml、女性为0.32～3.71 AU/ml；EBV-EA-IgA参考区间：20～70岁组为0.01～1.39 AU/ml、≥70岁组为0.01～1.52 AU/ml。结论　利用间接法为本实验室建立EB-VCA-IgA和EB-EA-IgA的生物参考区间，能为临床对鼻咽癌等EB病毒导致的疾病诊断提供更为准确的结果。

关键词:

EB病毒, 间接法, 生物参考区间, EB病毒衣壳抗原抗体, EB病毒早期抗原抗体