Abstract: Objective　To investigate the protective effect of microRNA-132 (miR-132) on PC12 cells and its possible mechanism. Methods　The research time was from September 2021 to March 2022. The PC12 cells in the logarithmic growth phase were treated with glutamate to establish excitatory injury models; the experimental group was transfected with miR-132 mimics by the transient transfection method, cultured for 24 h, and treated with glutamate for 24 h. CCK8 assay was used to detect the survival rate of the PC12 cells in each group; flow cytometry was used to detect reactive oxygen species (ROS) and malondialdehyde (MDA); enzyme-linked immunosorbent assay (ELISA) was used to detect interleukin (IL)-1β and IL-6. LSD-t test and χ² test were applied. Results　The expression level of miR-132 in the PC12 cells treated with glutamate was lower than that of the control group (P<0.01). The viability of the PC12 cells transfected with miR-132 mimics was higher than that after transfection with NC mimics (P<0.05). The level of oxidative stress after transfection of miR-132 mimics was lower than that of the NC mimics group (all P<0.05). miR-132 mimics cells down-regulated inflammatory factors. Conclusion　miR-132 can promote the proliferation of PC12 cells and inhibit the apoptosis of PC12 cells. The mechanism may be to inhibit oxidative stress and down-regulate the expressions of IL-1β and IL-6 related to inflammation.

Key words: Cerebral infarction, Neuronal cells, miR-132

摘要： 目的　探讨微小RNA-132（miR-132）对PC12细胞的保护作用及其可能的作用机制。方法　研究时间为2021年9月至2022年3月。取对数生长期PC12细胞，采用谷氨酸处理，建立兴奋性损伤模型；实验组采用瞬时转染法转染miR-132 mimics，培养24 h后加入谷氨酸处理24 h。采用CCK8法检测各组PC12细胞存活率；流式细胞术检测活性氧（reactive oxygen species，ROS）及丙二醛（MDA），酶联免疫吸附试验（ELISA）检测炎症因子白细胞介素（IL）-1β、IL-6的表达。采用LSD-t检验和χ²检验。结果　谷氨酸处理后的PC12细胞，miR-132表达水平低于对照组（P<0.01）。转染miR-132 mimics后PC12细胞的活力均高于转染NC mimics后（P<0.05）。转染miR-132 mimics后的氧化应激水平较NC mimics组降低（均P<0.05）。miR-132 mimics细胞炎症因子下调。结论　miR-132可促进PC12细胞的增殖、抑制PC12细胞凋亡，其机制可能为抑制氧化应激，下调炎症调节相关因子IL-1β、IL-6的表达。

关键词: 脑梗死, 神经细胞, miR-132