关键词: UGT1A1, 基因多态性, 妊娠女性, 甲状腺功能减退, 临床价值

Abstract:

Objective　To explore the clinical value of uridine 5′-diphospho-glucuronosyltransferase 1-1 (UGT1A1) gene polymorphism in pregnant women with hypothyroidism in Bao'an area. Methods　One hundred women with hypothyroidism during pregnancy treated at Shiyan People's Hospital from January 2021 to February 2022 were selected as an observation group, and 100 healthy women with normal thyroid function taking property inspection during the same period were selected as a control group. The fasting venous blood of all the subjects was taken for RNA extraction and sequencing. The observation group were routinely treated with levothyroxine tablets for 4 weeks. The serum levels of thyroid stimulating hormone (TSH) and thyroid peroxidase antibody (TPOAb) were detected. The χ², paired t, or F tests were applied. Results　In the observation group, there were 73 cases of UGT1A1*6 wild type, 23 cases of heterozygous mutation type (G/A), 4 cases of homozygous mutation type (A/A), 70 cases of UGT1A1*28 wild type, 27 cases of G/A type, and 3 cases of A/A type; in the control group, there were 69 cases of UGT1A1*6 wild type, 26 cases of G/A type, 5 cases of A/A type, 72 cases of UGT1A1*28 wild type, 26 cases of G/A type, and 2 cases of A/A type; there were no statistical differences in UGT1A1*6 and UGT1A1*28 gene polymorphisms between the observation group and the control group (both P>0.05). Before the treatment, there were no statistical differences in TSH and TPOAb levels between the patients with different UGT1A1*6 and UGT1A1*28 genotypes (all P>0.05); after the treatment, the TSH and TPOAb levels of the patients of UGT1A1*6 and UGT1A1*28 wild types were the highest, those of the patients of UGT1A1*6 G/A type were the second, and the TSH level of the patients of UGT1A1*6 A/A type was the lowest, with statistical differences (all P<0.05). Conclusions　The UGT1A1 gene polymorphism in women with hypothyroidism during pregnancy is similar to that in healthy pregnant women. The levels of TSH and TPOAb in patients with UGT1A1*6 and UGT1A1*28 homozygous mutant hypothyroidisms during pregnancy after clinical treatment are significantly reduced. Clinically, it should consider to give an appropriate dose of drug treatment according to the UGT1A1 genotypes.

Key words: UGT1A1, Gene polymorphism, Pregnant women, Hypothyroidism, Clinical value