MALDI-TOF MS在碳青霉烯耐药肺炎克雷伯菌同源性分析中的应用

doi:10.3760/cma.j.cn441417-20240101-14023

国际医药卫生导报 ›› 2025, Vol. 31 ›› Issue (14): 2402-2407.DOI: 10.3760/cma.j.cn441417-20240101-14023

MALDI-TOF MS在碳青霉烯耐药肺炎克雷伯菌同源性分析中的应用

高芳琳阮海涛詹文芳

福建中医药大学附属人民医院检验科，福州 350004

收稿日期:2025-01-20 出版日期:2025-07-01 发布日期:2025-08-06
通讯作者: 高芳琳，Email：13559189164@139.com
基金资助:
福建省教育厅2021年度中青年教师教育科研项目（JAT210207）；福建省科技厅2024年度省自然科学基金联合资助面上项目（2024J01766）

Application of MALDI-TOF MS in homology analysis of carbapenem-resistant Klebsiella pneumoniae

Gao Fanglin, Ruan Haitao, Zhan Wenfang

Department of Clinical Laboratory, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou 350004, China

Received:2025-01-20 Online:2025-07-01 Published:2025-08-06
Contact: Gao Fanglin, Email: 13559189164@139.com
Supported by:
Education and Scientific Research Project for Middle-aged and Young Teachers Supported by Fujian Education Department in 2021 (JAT210207); Jointly Funded General Project of Natural Science Foundation of Fujian Department of Science and Technology in 2024 (2024J01766)

摘要/Abstract

摘要：

目的　探讨基质辅助激光解析电离飞行时间质谱（matrix-assisted laser desorption/ionization time-of-flight mass spectrometry，MALDI-TOF MS）检测耐碳青霉烯类肺炎克雷伯菌（carbapenem-resistant Klebsiella pneumoniae，CRKP）同源性的应用价值。方法　收集2024年5月至6月福建中医药大学附属人民医院ICU同一患者痰、尿、血分离的3株CRKP，分别采用MALDI-TOF MS与单核苷酸多态性（single nucleotide polymorphisms，SNP）差异位点变异检测对3株CRKP进行同源性分析。结果　MALDI-TOF MS同源性分析结果显示，3株CRKP中分离自痰和血的CRKP1与CRKP3同源性更高，表明该患者血液检出的CRKP3可能来源于呼吸道，该结果与SNP差异位点变异检测的同源性分析结果一致。结论　MALDI-TOF MS能够对同一患者不同来源菌株进行同源性分析，实现菌株溯源，对临床实现快速诊断具有重要意义。

关键词: CRKP, MALDI-TOF MS, 单核苷酸多态性检测, 同源性分析

Abstract:

Objective　To Explore the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in the homology analysis of carbapenem-resistant Klebsiella pneumoniae (CRKP). Methods　Three strains of CRKP isolated from the sputum, urine, and blood of the same patient in ICU, People's Hospital Affiliated to Fujian University of Traditional Chinese Medicine from May to June 2024 were collected. Homology analysis of the three strains of CRKP was performed using MALDI-TOF MS and detection of single nucleotide polymorphisms (SNP) variation sites. Results　The homology analysis results of MALDI-TOF MS showed that CRKP1 isolated from the sputum had a higher homology with CRKP3 isolated from the blood, indicating that the CRKP3 detected in the patient's blood might originate from the respiratory tract. This result was consistent with the homology analysis results based on SNP variation detection. Conclusions　MALDI-TOF MS is capable of performing homology analysis on strains from different sources in the same patient, enabling strain tracing. It is of significant importance for achieving rapid diagnosis in clinical settings.

Key words: CRKP, , MALDI-TOF MS, , Single nucleotide polymorphisms detection, , Homology analysis

高芳琳阮海涛詹文芳.

MALDI-TOF MS在碳青霉烯耐药肺炎克雷伯菌同源性分析中的应用 [J]. 国际医药卫生导报, 2025, 31(14): 2402-2407.

Gao Fanglin, Ruan Haitao, Zhan Wenfang.

Application of MALDI-TOF MS in homology analysis of carbapenem-resistant Klebsiella pneumoniae [J]. International Medicine and Health Guidance News, 2025, 31(14): 2402-2407.

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MALDI-TOF MS在碳青霉烯耐药肺炎克雷伯菌同源性分析中的应用

Application of MALDI-TOF MS in homology analysis of carbapenem-resistant Klebsiella pneumoniae

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