绒毛组织Bcl-2、Bax、LC3、P62与稽留流产
患者解脲支原体感染的关系

doi:10.3760/cma.j.cn441417-20240430-01003

国际医药卫生导报 ›› 2025, Vol. 31 ›› Issue (1): 12-16.DOI: 10.3760/cma.j.cn441417-20240430-01003

绒毛组织Bcl-2、Bax、LC3、P62与稽留流产患者解脲支原体感染的关系

杨杰郭丽宁王彩丽

榆林市第一医院妇产科，榆林 719000

收稿日期:2024-04-30 出版日期:2025-01-01 发布日期:2025-01-13
通讯作者: 王彩丽，Email：645760298@qq.com
基金资助:
目的　研究绒毛组织 B 淋巴细胞瘤-2基因（Bcl-2）、B 淋巴细胞瘤-2相关 X 蛋白（Bax）、微管相关蛋白 1轻链 3（LC3）、P62对稽留流产患者解脲支原体感染的预测价值。方法　本研究为队列研究。选取 2022 年 1 月至 2023 年 6 月榆林市第一医院收治的稽留流产患者 156 例作为稽留流产组，并选取同期在本院进行人工流产的患者170例作为人工流产组。人工流产组年龄、孕次、产次、既往流产次数、妊娠时间分别为（28.61±5.30）岁、（1.54±0.48）次、（0.97±0.24）次、（0.87±0.25）次、（53.72± 4.49）d，稽留流产组分别为（29.18±5.57）岁、（1.49±0.43）次、（0.95±0.26）次、（0.91±0.32）次、（54.55± 4.20）d。比较两组解脲支原体感染率及绒毛组织 Bcl-2、Bax、LC3、P62表达情况。稽留流产患者根据是否发生解脲支原体感染再分为阴性组（96例）、阳性组（60例），比较两组绒毛组织 Bcl-2、Bax、LC3、 P62表达情况，采用受试者操作特征曲线（ROC）分析绒毛组织Bcl-2、Bax、LC3、P62表达对稽留流产患者解脲支原体感染的预测价值。采用独立样本t检验、χ2 检验进行统计学分析。结果　稽留流产组解脲支原体阳性率高于人工流产组［38.46%（60/156）比12.94%（22/170）］（P<0.05）。稽留流产组绒毛组织Bcl-2、P62表达水平低于人工流产组，Bax、LC3表达水平高于人工流产组（均P<0.05）。阳性组绒毛组织 Bcl-2、P62 表达水平低于阴性组，Bax、LC3 表达水平高于阴性组（均 P<0.05）。绒毛组织 Bcl-2、 Bax、LC3、P62联合预测稽留流产患者解脲支原体感染的曲线下面积（AUC）为0.852，高于四者单独检测（均P<0.05）。结论　与人工流产患者相比，稽留流产患者解脲支原体感染发生率较高，且两组绒毛组织 Bcl-2、Bax、LC3、P62 表达存在明显差异。解脲支原体感染可引起绒毛组织 Bcl-2、Bax、LC3、 P62表达差异，且四者联合提高对稽留流产患者解脲支原体感染的预测价值。

Relationships between the expressions of Bcl-2, Bax, LC3, and P62 in chorionic villi and Ureaplasma urealyticum infection in patients with missed abortion

Yang Jie, Guo Lining, Wang Caili

Department of Gynecology and Obstetrics, Yulin First Hospital, Yulin 719000, China

Received:2024-04-30 Online:2025-01-01 Published:2025-01-13
Contact: Wang Caili, Email:645760298@qq.com
Supported by:
Objective To study the predictive values of B lymphoblastoma -2 gene (Bcl-2), B lymphocytoma -2 related X protein (Bax), microtubule-associated protein 1 light chain 3 (LC3), and P62 in chorionic villi for Ureaplasma urealyticum infection in patients with missed abortion. Methods It was a cohort study. A total of 156 missed abortion patients admitted to Yulin First Hospital from January 2022 to June 2023 were selected as the missed abortion group, and 170 patients undergoing induced abortion in our hospital during the same period were selected as the induced abortion group. In the induced abortion group, the age, pregnancy times, delivery times, previous abortion times, and pregnancy time were (28.61±5.30) years old, (1.54±0.48) times, (0.97±0.24) times, (0.87±0.25) times, and (53.72±4.49) days, and those in the missed abortion group were (29.18±5.57) years old, (1.49±0.43) times, (0.95±0.26) times, (0.91±0.32) times, and (54.55±4.20) days, respectively. The rate of Ureaplasma urealyticum infection and the expressions of Bcl-2, Bax, LC3, and P62 in chorionic villi were compared between the two groups. The patients with missed abortion were divided into a negative group (96 cases) and a positive group (60 cases) according to whether Ureaplasma urealyticum infection occurred, and the expressions of Bcl-2, Bax, LC3, and P62 in chorionic villi were compared between the two groups. The predictive values of Bcl-2, Bax, LC3, and P62 in chorionic villi for Ureaplasma urealyticum infection in patients with missed abortion were analyzed by the receiver operating characteristic curve (ROC). Independent sample t test and χ2 test were used for statistical analysis. Results The positive rate of Ureaplasma urealyticum in the missed abortion group was higher than that in the induced abortion group [38.46% (60/156) vs. 12.94% (22/170)] (P<0.05). The expression levels of Bcl-2 and P62 in chorionic villi of the missed abortion group were lower than those of the induced abortion group, while the expression levels of Bax and LC3 were higher than those of the induced abortion group (all P<0.05). The expression levels of Bcl-2 and P62 in chorionic villi of the positive group were lower than those of the negative group, while the expression levels of Bax and LC3 were higher than those of the negative group (all P<0.05). The area under the curve (AUC) of the combination of Bcl-2, Bax, LC3, and P62 in chorionic villi to predict Ureaplasma urealyticum infection in missed abortion patients was 0.852, which was higher than that of the four alone (all P<0.05). Conclusions Compared with induced abortion patients, the incidence of Ureaplasma urealyticum infection was higher in missed abortion patients, and the expressions of Bcl-2, Bax, LC3, and P62 in chorionic villi were significantly different between the two groups. Ureaplasma urealyticum infection could cause differences in the expressions of Bcl-2, Bax, LC3, and P62 in chorionic villi, and the combination of the four could improve the prediction value for Ureaplasma urealyticum infection in missed abortion patients.

摘要/Abstract

摘要： 目的　研究绒毛组织 B 淋巴细胞瘤-2基因（Bcl-2）、B 淋巴细胞瘤-2相关 X 蛋白（Bax）、微管相关蛋白 1轻链 3（LC3）、P62对稽留流产患者解脲支原体感染的预测价值。方法　本研究为队列研究。选取 2022 年 1 月至 2023 年 6 月榆林市第一医院收治的稽留流产患者 156 例作为稽留流产组，并选取同期在本院进行人工流产的患者170例作为人工流产组。人工流产组年龄、孕次、产次、既往流产次数、妊娠时间分别为（28.61±5.30）岁、（1.54±0.48）次、（0.97±0.24）次、（0.87±0.25）次、（53.72± 4.49）d，稽留流产组分别为（29.18±5.57）岁、（1.49±0.43）次、（0.95±0.26）次、（0.91±0.32）次、（54.55± 4.20）d。比较两组解脲支原体感染率及绒毛组织 Bcl-2、Bax、LC3、P62表达情况。稽留流产患者根据是否发生解脲支原体感染再分为阴性组（96例）、阳性组（60例），比较两组绒毛组织 Bcl-2、Bax、LC3、 P62表达情况，采用受试者操作特征曲线（ROC）分析绒毛组织Bcl-2、Bax、LC3、P62表达对稽留流产患者解脲支原体感染的预测价值。采用独立样本t检验、χ2 检验进行统计学分析。结果　稽留流产组解脲支原体阳性率高于人工流产组［38.46%（60/156）比12.94%（22/170）］（P<0.05）。稽留流产组绒毛组织Bcl-2、P62表达水平低于人工流产组，Bax、LC3表达水平高于人工流产组（均P<0.05）。阳性组绒毛组织 Bcl-2、P62 表达水平低于阴性组，Bax、LC3 表达水平高于阴性组（均 P<0.05）。绒毛组织 Bcl-2、 Bax、LC3、P62联合预测稽留流产患者解脲支原体感染的曲线下面积（AUC）为0.852，高于四者单独检测（均P<0.05）。结论　与人工流产患者相比，稽留流产患者解脲支原体感染发生率较高，且两组绒毛组织 Bcl-2、Bax、LC3、P62 表达存在明显差异。解脲支原体感染可引起绒毛组织 Bcl-2、Bax、LC3、 P62表达差异，且四者联合提高对稽留流产患者解脲支原体感染的预测价值。

关键词: 　稽留流产, 解脲支原体, B 淋巴细胞瘤-2 基因, B 淋巴细胞瘤-2 相关 X 蛋白, 微管相关蛋白1轻链3, P62

Abstract: Objective To study the predictive values of B lymphoblastoma -2 gene (Bcl-2), B lymphocytoma -2 related X protein (Bax), microtubule-associated protein 1 light chain 3 (LC3), and P62 in chorionic villi for Ureaplasma urealyticum infection in patients with missed abortion. Methods It was a cohort study. A total of 156 missed abortion patients admitted to Yulin First Hospital from January 2022 to June 2023 were selected as the missed abortion group, and 170 patients undergoing induced abortion in our hospital during the same period were selected as the induced abortion group. In the induced abortion group, the age, pregnancy times, delivery times, previous abortion times, and pregnancy time were (28.61±5.30) years old, (1.54±0.48) times, (0.97± DOI：10.3760/cma.j.cn441417-20240430-01003 收稿日期 2024-04-30 本文编辑吴相思引用本文：杨杰, 郭丽宁, 王彩丽. 绒毛组织Bcl-2、Bax、LC3、P62与稽留流产患者解脲支原体感染的关系 [J]. 国际医药卫生导报, 2025, 31(1): 12-16. DOI: 10.3760/cma.j.cn441417-20240430-01003. · 12 · 国际医药卫生导报 2025 年1 月第 31 卷第 1 期　IMHGN, January 2025, Vol. 31, No. 1 0.24) times, (0.87±0.25) times, and (53.72±4.49) days, and those in the missed abortion group were (29.18±5.57) years old, (1.49±0.43) times, (0.95±0.26) times, (0.91±0.32) times, and (54.55±4.20) days, respectively. The rate of Ureaplasma urealyticum infection and the expressions of Bcl-2, Bax, LC3, and P62 in chorionic villi were compared between the two groups. The patients with missed abortion were divided into a negative group (96 cases) and a positive group (60 cases) according to whether Ureaplasma urealyticum infection occurred, and the expressions of Bcl-2, Bax, LC3, and P62 in chorionic villi were compared between the two groups. The predictive values of Bcl-2, Bax, LC3, and P62 in chorionic villi for Ureaplasma urealyticum infection in patients with missed abortion were analyzed by the receiver operating characteristic curve (ROC). Independent sample t test and χ2 test were used for statistical analysis. Results The positive rate of Ureaplasma urealyticum in the missed abortion group was higher than that in the induced abortion group [38.46% (60/156) vs. 12.94% (22/170)] (P<0.05). The expression levels of Bcl-2 and P62 in chorionic villi of the missed abortion group were lower than those of the induced abortion group, while the expression levels of Bax and LC3 were higher than those of the induced abortion group (all P<0.05). The expression levels of Bcl-2 and P62 in chorionic villi of the positive group were lower than those of the negative group, while the expression levels of Bax and LC3 were higher than those of the negative group (all P<0.05). The area under the curve (AUC) of the combination of Bcl-2, Bax, LC3, and P62 in chorionic villi to predict Ureaplasma urealyticum infection in missed abortion patients was 0.852, which was higher than that of the four alone (all P<0.05). Conclusions Compared with induced abortion patients, the incidence of Ureaplasma urealyticum infection was higher in missed abortion patients, and the expressions of Bcl-2, Bax, LC3, and P62 in chorionic villi were significantly different between the two groups. Ureaplasma urealyticum infection could cause differences in the expressions of Bcl-2, Bax, LC3, and P62 in chorionic villi, and the combination of the four could improve the prediction value for Ureaplasma urealyticum infection in missed abortion patients.

Key words: Missed abortion, Ureaplasma urealyticum, B lymphoblastoma -2 gene, B lymphocytoma -2 related X protein, Microtubule-associated protein 1 light chain 3, P62

杨杰郭丽宁王彩丽. 绒毛组织Bcl-2、Bax、LC3、P62与稽留流产患者解脲支原体感染的关系[J]. 国际医药卫生导报, 2025, 31(1): 12-16.

Yang Jie, Guo Lining, Wang Caili. Relationships between the expressions of Bcl-2, Bax, LC3, and P62 in chorionic villi and Ureaplasma urealyticum infection in patients with missed abortion [J]. International Medicine and Health Guidance News, 2025, 31(1): 12-16.

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绒毛组织Bcl-2、Bax、LC3、P62与稽留流产患者解脲支原体感染的关系

Relationships between the expressions of Bcl-2, Bax, LC3, and P62 in chorionic villi and Ureaplasma urealyticum infection in patients with missed abortion

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