关键词:

癫痫, 儿童, 高迁移率族蛋白B1, 水通道蛋白4, 8-羟基脱氧鸟苷, 脂联素, 疾病进展, 认知功能

Abstract:

Objective　To investigate the relationships between serum high mobility group protein B1 (HMGB1), aquaporin 4 (AQP4), 8-hydroxydeoxyguanosine (8-OHdG), and adiponectin (APN) levels and disease progression and cognitive function in children with epilepsy. Methods　A total of 142 children with epilepsy admitted to the Second People's Hospital of Liaocheng from January 2021 to December 2023 were selected as the study group, including 74 boys and 68 girls, aged (9.08±0.80) years. According to the type of epilepsy, they were divided into a general tonic-clonic seizure group (55 cases) and a focal seizure group (87 cases); they were divided into a cognitive dysfunction group (51 cases) and a normal cognitive function group (91 cases) according to the cognitive function. In addition, 145 normal children who received health checkups during the same period were selected as the control group. The clinical data of the selected children were collected, and serum HMGB1, AQP4, 8-OHdG, and APN levels were detected. Independent sample t test was used to compare serum HMGB1, AQP4, 8-OHdG, and APN levels between the study group and the control group, as well as between children with different types of epilepsy and different cognitive functions. Pearson correlation analysis was used to analyze the correlations between serum HMGB1, AQP4, 8-OHdG, and APN levels and cognitive function in children with epilepsy. The receiver operating characteristic curve (ROC) was plotted, and the area under the curve (AUC) was obtained. The predictive value of serum HMGB1, AQP4, 8-OHdG, and APN alone and in combination for cognitive dysfunction in children with epilepsy was analyzed. Results　The levels of serum HMGB1 [(5.01±1.22) μg/L] and 8-OHdG [(0.43±0.10) μg/L] in the study group were higher than those in the control group [(2.91±0.86) μg/L and (0.29±0.08) μg/L] (both P<0.05), but the levels of serum AQP4 [(56.79±15.02) μg/L] and APN [(6.77±1.45) mg/L] were lower than those in the control group [(163.58±31.27) μg/L and (10.13±2.24) mg/L] (both P<0.05). The levels of serum HMGB1 [(6.14±1.98) μg/L] and 8-OHdG [(0.53±0.15) μg/L] in the general tonic-clonic seizure group were higher than those in the focal seizure group [(3.95±1.10) μg/L and (0.36±0.09) μg/L] (both P<0.05), but the levels of serum AQP4 [(41.43±12.07) μg/L] and APN [(4.64±1.40) mg/L] were lower than those in the focal seizure group [(70.89±21.44) μg/L and (7.98±2.32) mg/L] (both P<0.05). The levels of serum HMGB1 [(6.18±2.04) μg/L] and 8-OHdG [(0.57±0.19) μg/L] in the cognitive dysfunction group were higher than those in the normal cognitive function group [(3.98±1.25) μg/L and (0.38±0.12) μg/L] (both P<0.05), but the levels of serum AQP4 [(46.94±15.15) μg/L] and APN [(4.97±1.55) mg/L] were lower than those in the normal cognitive function group [(71.43±23.59) μg/L and (8.01±2.53) mg/L] (both P<0.05). The levels of serum HMGB1 and 8-OHdG were negatively correlated with cognitive function in children with epilepsy (r=-0.499 and -0.504, both P<0.05), but the levels of serum AQP4 and APN were positively correlated with cognitive function (r=0.531 and 0.527, both P<0.05). The AUC of combined detection of serum HMGB1, AQP4, 8-OHdG, and APN was 0.920, which was higher than that of single detection of each index (0.751, 0.833, 0.817, and 0.848). Conclusions　Serum HMGB1 and 8-OHdG were highly expressed in children with epilepsy, while serum AQP4 and APN were lowly expressed. All four of them were involved in the process of disease progression in children, and were closely related to cognitive dysfunction in children. The combination of all four of them had a higher predictive value for cognitive dysfunction in children with epilepsy.

Key words:

Epilepsy,  , Children, High mobility group protein B1, Aquaporin 4, 8-hydroxydeoxyguanosine, Adiponectin, Disease progression,  , Cognitive function