关键词:

EB病毒, 间接法, 生物参考区间, EB病毒衣壳抗原抗体, EB病毒早期抗原抗体

Abstract:

Objective　The biological reference intervals of epstein-barr virus capsid antigen antibody (EBV-VCA-IgA) and epstein-barr virus early antigen antibody (EBV-EA-IgA) in our laboratory were established by an indirect method using the data from the laboratory information management system (LIS) of Guangdong Second Traditional Chinese Medicine Hospital. Methods　The results of EBV-VCA-IgA and EBV-EA-IgA in 2 160 healthy people (998 males and 1 162 females) who were 20 to 100 years old with normal aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (T-BILI), direct bilirubin (D-BILI), indirect bilirubin (I-BILI), creatinine (CR), and urea nitrogen (BUN) were retrospectively analyzed by the LIS data. The results were grouped by gender and age. Kolmogorov Smirnov test (K-S test) was used to test the normality of each group. When P>0.05, it was regarded as normal distribution; when P<0.05, it was regarded as non-normal distribution. For non-normal distribution data, the data larger than P₂₅-3×IQR and less than P₇₅+3×IQR were excluded. The non-parametric test was used to analyze the data after elimination. The statistical differences between the groups were analyzed. The biological reference intervals of EBV-VCA-IgA and EBV-EA-IgA were established by the P_2.5-P_97.5 intervals, and the reference intervals were verified. Results　There was a statistical difference in the EBV-VCA-IgA reference interval between the males and the females (P<0.001), but no statistical difference between the age groups (P=0.087). There was no statistical difference in the EBV-EA-IgA reference interval between the males and the females (P=0.127), but was between the age groups (P=0.004). The EBV-EA-IgA reference intervals in the 20-50 years old group and the ≥50-70 years old group were statistically different from that in the ≥70 years old group (P=0.002 and <0.001). There was no statistical difference in the EBV-EA-IgA reference interval between the 20-50 years old group and the ≥50-70 years old group (P=0.043), so these two groups were united as one group (20-70 years old) to build a reference interval, and it was statistically different from that in the ≥70 years old group (P=0.001). Accordingly, the EBV-VCA-IgA reference interval was 0.34-3.88 AU/ml in the males, and was 0.32-3.71 AU/ml in the females; the EBV- EA-IgA reference interval was 0.01-1.39 AU/ml in the 20-70 years old group, and was 0.01-1.52 AU/ml in the ≥70 years old group. Conclusion　The indirect method was used to establish the biological reference intervals of EBV-VCA-IgA and EBV-EA-IgA in our laboratory, which could provide more accurate results for the clinical diagnosis of diseases caused by EB virus, such as nasopharyngeal carcinoma.

Key words:

Epstein-barr virus, Indirect method, Biological reference interval, Epstein-barr virus capsid antigen antibody, Epstein-barr virus early antigen antibody