关键词:

外阴阴道假丝酵母菌病, 红核妇洁洗液, 作用机制

Abstract:

Objective　To explore the mechanism of Honghe Fujie liquid in treating vulvovaginal candidiasis (VVC). Methods　Sixty SPF-grade SD rats (body weight 180-220 g) were randomly divided into a blank group, a model group, an amphotericin B effervescent tablet group, and low, moderate, and high dosages of Honghe Fujie liquid groups, with 10 rats in each group. Except for the blank group, the VVC rat models were established in the other groups by the subcutaneous injection of 0.2 g of estradiol valerate and intravaginal injection of 20 μl of Candida albicans suspension (5×10⁷ cfu/ml). The rats in the drug groups were administered the corresponding drngs vaginally, while the blank group and the model group were given normal saline, once a day for 7 days. Twenty-four hours after the last administration, the rats were anesthetized, and vaginal tissues were taken for hematoxylin-eosin (HE) staining to observe the pathological changes in the rats' tissues and to analyze the repair of the vaginal mucosa by the Honghe Fujie liquid. The transmission electron microscopy was used to observe the changes in the morphology of Candida albicans after the treatment with the Honghe Fujie liquid. The real-time quantitative PCR (qRT-PCR) was used to detect the expressions of HWP1 and ECE1 genes that enhance the virulence of Candida albicans, the ALS3 gene that promotes adhesion of Candida albicans, and the ECE1, EFG1, ERG1, ERG7 genes that regulate hyphal growth and the integrity of the cell membrane. Data analysis and mapping were performed using GraphPad Prism 8.0; Student's t test was used for comparison between groups. Image data was analyzed using the Image J(NIH) software. Results　Compared with that in the blank group, the model group rats had obvious edema and hyperplasia, epithelial cell shedding, infiltration by a large number of inflammatory cells in the vaginal tissue. Compared with the in the model group, the vaginal tissue lesions of rats in each drug group were improved to varying degrees. The results of transmission electron microscopy suggested that Honghe Fujie liquid could destroy the morphology and structure of bacteria, leading to the leakage of cytoplasmic contents of bacteria, and causing the death of bacteria. The qRT-PCR results showed that Honghe Fujie liquid could downregulate the virulence-enhancing HWP1 and ECE1 genes (P<0.05 or P<0.01), and promote the downregulation of ALS3 gene, which promotes mycelial adhesion, and ECE1, EFG1, ERG1, and ERG7 genes, regulates mycelial growth, and affects the integrity of the fungal plasma membrane. Conclusion　Honghe Fujie liquid has significant antibacterial effect on Candida albicans, which may be related to its ability to regulate the expressions of the genes related to fungal virulence, hyphal adhesion, and the completeness of the fungal cell membrane, as well as its ability to destroy the structure of mature biofilms and promote the entry of drugs into the biofilm to exert a comprehensive bactericidal action.

Key words:

Vulvovaginaliasis candidiasis, Honghe Fujie liquid, Action mechanism