Abstract:

Objective　To investigate the clinical characteristics and genotypes of human papilloma virus (HPV) of non-genital cutaneous warts. Methods　One hundred and sixteen patients with non-genital cutaneous warts treated at The First Affiliated Hospital of Sun Yat-sen University from July 2019 to November 2020 were enrolled for the cohort study, including 63 males and 53 females who were (38.00±12.41) years old. The clinical data were collected; the DNA was harvested from the warts samples; the common primers were used to amplify the DNA. According to the 11 common HPV genotypes by gene sequencing of specific primers, the characteristics of the HPV genotypes and the clinical features of the cutaneous warts were analyzed. The chi-square test, independent samples t test, and one-way ANOVA were used. Results　HPV DNA was detected in 90.50% patients (105/116). The proportions of plantar warts, common warts, and flat warts were 51.43% (54/105), 32.40% (34/105) and 16.20% (17/105), respectively. The most predominantly prevalent genotypes were HPV 1 (53.33%), HPV 27 (37.14%), HPV 2 (29.52%), and HPV 4 (23.81%). The proportion of double or multiple genotype infection was 54.29%; the proportion was higher in hand and foot warts. The common multiple HPV genotypes were HPV 1+4, HPV 1+2, HPV 1+27, HPV 2+27, and HPV 1+4+27. High risk HPV was detected in 3 cases of common warts (HPV 18) and 1 case of flat wart (HPV 58). Conclusions　The prevalence characteristics of HPV genotypes in non-genital cutaneous warts in Guangdong may be distinctive from other areas. Multiple genotype HPV infection is common, especially in hand and foot warts.

Key words:

Non-genital cutaneous warts, Human papillomavirus, Genotype

摘要：

目的　探讨非生殖器皮肤疣人类乳头状瘤病毒（human papilloma virus，HPV）分型特点及临床特征。方法　入组2019年7月至2020年11月中山大学附属第一医院116例非生殖器皮肤疣患者开展队列研究，男63例，女53例，年龄（38.00±12.41）岁。采集临床资料，取疣体组织提取DNA，采用通用引物扩增DNA，结合11种型特异性引物扩增测序分型，分析不同类型皮肤疣的HPV型别特点，以及不同部位皮肤疣的临床特征。采用卡方检验、独立样本t检验和单因素方差分析。结果　HPV阳性率90.50%（105/116），跖疣、寻常疣、扁平疣分别占51.43%（54/105）、32.40%（34/105）和16.20%（17/105）。HPV型别以HPV 1（53.33%）、HPV 27（37.14%）、HPV 2（29.52%）和HPV 4（23.81%）为主；双重或多重型别HPV感染57例，占54.29%，手足部疣比例更高，以HPV 1+4、HPV 1+2、HPV 1+27、HPV 2+27、HPV 1+4+27等为主。3例寻常疣中检出高危型HPV 18，1例扁平疣中检出高危型HPV 58。结论　广东地区非生殖器皮肤疣常见HPV型别与国外及国内其他地区不尽相同，且双重或多重HPV感染较多，手足部更明显。

关键词:

非生殖器皮肤疣, 人乳头状瘤病毒, 基因型