Abstract: Objective　To explore the mechanism of microRNA (miR)-3529-3p in inhibiting the proliferation of ovarian cancer SKOV-3 cells. Methods　This study was from January to May 2021. NC mimic (NC group) and miR-3529-3p mimic (miR-3529-3p group) were respectively transfected into the ovarian cancer SKOV-3 cells. Real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the transfection efficiency. The CCK-8 method was used to detect the optical density value of each group. The colony formation test was used to detect the number of colonies formed in each group. The miRNAMap database was used to predict the target gene of miR-3529-3p. qRT-PCR and Western blot were used to detect the expression of target gene. Independent-sample t test was used. Results　The expressions of miR-3529-3p in the SKOV-3 cells in the miR-3529-3p group and the NC group were (1.01±0.07) and (9.55±1.50), respectively. The expression of miR-3529-3p in the NC group was significantly lower than that in the miR-3529-3p group (t=5.68, P<0.01). The CCK-8 method showed that miR-3529-3p overexpression inhibited the proliferation of the SKOV-3 cells (P<0.05). Compared with that in the NC group, the number of colonies formed in the miR-3529-3p group was significantly reduced (P<0.05). The target gene of miR-3529-3p might be E2F transcription factor 3 (E2F3). Compared with that in the NC group, the expression of E2F3 gene in the SKOV-3 cells in the miR-3529-3p group was significantly reduced (P<0.01). Conclusions　miR-3529-3p can inhibit the proliferation of ovarian cancer SKOV-3 cells, and its possible molecular mechanism is achieved by targeting and inhibiting the expression of E2F3.

Key words: Ovarian cancer, miR-3529-3p, E2F transcription factor 3, Cell proliferation

摘要： 目的　探讨微小RNA（miR）-3529-3p抑制卵巢癌SKOV-3细胞增殖的作用机制。方法　研究时间为2021年1月至5月。分别转染NC mimic（NC组）和miR-3529-3p mimic（miR-3529-3p组）至卵巢癌SKOV-3细胞。实时荧光定量逆转录聚合酶链反应（qRT-PCR）检测转染效率。CCK-8法检测每组细胞的光密度值。集落形成实验检测每组细胞的集落形成数。miRNAMap数据库预测miR-3529-3p的靶基因。qRT-PCR和蛋白质免疫印迹法（Western blot）检测靶基因的表达。采用独立样本t检验。结果　miR-3529-3p组和NC组SKOV-3细胞中miR-3529-3p表达分别为（1.01±0.07）和（9.55±1.50），NC组miR-3529-3p表达显著低于miR-3529-3p组（t=5.68，P<0.01）。CCK-8法显示miR-3529-3p过表达抑制SKOV-3细胞增殖（P<0.05）。与NC组相比，miR-3529-3p组集落形成数显著减少（P<0.05）。miR-3529-3p的靶基因可能是E2F转录调节因子3（E2F transcription factor 3，E2F3）。与NC组比较，miR-3529-3p组SKOV-3细胞中E2F3基因表达明显降低（P<0.01）。结论　miR-3529-3p可抑制卵巢癌SKOV-3细胞的增殖，其可能的分子机制是通过靶向抑制E2F3表达实现。

关键词: 卵巢癌, miR-3529-3p, E2F转录调节因子3, 细胞增殖